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The map label for this gene is gloB [H]

Identifier: 157159673

GI number: 157159673

Start: 233626

End: 234381

Strand: Reverse

Name: gloB [H]

Synonym: EcHS_A0216

Alternate gene names: 157159673

Gene position: 234381-233626 (Counterclockwise)

Preceding gene: 157159675

Following gene: 157159672

Centisome position: 5.05

GC content: 39.81

Gene sequence:

>756_bases
ATGAATCTTAACAGTATTCCCGCCTTTGATGACAATTACATCTGGGTTTTGAATGATGAAGCAGGTCGCTGCCTGATTGT
CGATCCCGGAGACGCAGAGCCAGTATTAAACGCCATTACCGCCAATAACTGGCAACCGGAGGCCATATTTCTCACCCACC
ATCATCACGATCACGTTGGCGGCGTAAAAGAACTGGTGGAAAAGTTTCCGCAAATTGTGGTGTATGGTCCACAAGAGACA
CAAGATAAGGGAACAACACAGGTAGTCAAAGATGGCGAAACTGCCTTCGTTTTGGGGCATGAATTTAGTGTAATTGCCAC
ACCGGGTCACACTTTAGGACATATCTGTTACTTCAGTAAACCTTATCTATTTTGCGGCGACACGCTGTTTTCTGGTGGGT
GTGGTAGGTTGTTTGAAGGGACAGCATCACAAATGTATCAATCACTTAAAAAGTTAAGTGCGTTACCTGACGATACATTG
GTATGTTGTGCTCATGAATATACGTTATCAAATATGAAGTTTGCTTTGAGTATTCTTCCGCACGATTTGTCCATAAATGA
TTATTATCGTAAAGTTAAGGAGTTACGGGCAAAAAATCAAATAACACTACCCGTAATTCTGAAAAATGAGCGGCAAATTA
ATGTTTTTTTAAGAACAGAAGATATTGATTTAATTAATGTAATTAATGAAGAAACATTATTGCAACAACCTGAAGAACGT
TTTGCATGGTTAAGGTCAAAGAAAGATAGGTTCTGA

Upstream 100 bases:

>100_bases
CTCCCCAGGGCAAATCGCCCCAGCAATCAGGAGCCACGACAGTTTGAGGGACTCTTGCCGGTTTCATCACAACCTTCCGT
TTCACACTAAGAGGTAATCT

Downstream 100 bases:

>100_bases
TAAAACTTTCTTGTCATCGGCTCCGTTCGCCGTTATGATCGGTCGTCTTTTAAGCAACTATTGACACACACATGAAGGCA
AAAGCGATATTACTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF

Sequences:

>Translated_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF
>Mature_251_residues
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNWQPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQET
QDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=34.2307692307692, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=34.2307692307692, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI14150041, Length=229, Percent_Identity=35.3711790393013, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI116642887, Length=232, Percent_Identity=35.3448275862069, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21703352, Length=232, Percent_Identity=35.3448275862069, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=37.3737373737374, Blast_Score=121, Evalue=5e-28,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=99.601593625498, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=30.0518134715026, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=227, Percent_Identity=32.5991189427313, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320478, Length=231, Percent_Identity=33.7662337662338, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=231, Percent_Identity=29.8701298701299, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=35.632183908046, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28465; Mature: 28465

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEECCCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC
>Mature Secondary Structure
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNWQPEAIFLTHHHHDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCEEEEECCCCHHEEEEEECC
PYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTLVCCAHEYTLSNMKFALSILP
CEEEECCCHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEEECC
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
CCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCEEEECCCHHHHHCCHHHH
FAWLRSKKDRF
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA