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The map label for this gene is yafC [H]

Identifier: 157159669

GI number: 157159669

Start: 229345

End: 230259

Strand: Reverse

Name: yafC [H]

Synonym: EcHS_A0212

Alternate gene names: 157159669

Gene position: 230259-229345 (Counterclockwise)

Preceding gene: 157159672

Following gene: 157159666

Centisome position: 4.96

GC content: 49.51

Gene sequence:

>915_bases
ATGAAAGCCACGTCGGAAGAACTCGCCATTTTTGTTTCGGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCAGTGAAAAAGCTGGAGATGAAACTTGGCGTTAGCCTGCTTAATCGGACCA
CGCGACAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCGTCGCGTACAGTCAATTTTGCAGGAGATGGCAGCGGCA
GAATCAGAAATTATGGAGACGCGTAATACACCGCGTGGACTGTTGCGCATCGATGCCGCAACTCCAGTGGTGCTGCACTT
TCTGATGCCGTTGATTAAGCCTTTCCGTGAACGCTATCCGGAAGTCACTTTGTCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATCGAAAGAAAAGTTGATGTCGCGATACGCGCCGGTACGTTAACGGATTCCAGCTTACGTGCCAGGCCGTTATTT
AATAGTTATCGAAAAATTATCGCCTCCCCCGATTATATTTCCCGCTACGGGAAGCCAGAAACGATCGACGATTTAAAGCA
ACATGTTTGCCTGGGATTCACTGAACCCGCTTCCCTCAATACCTGGCCGATAGCCTGTAGTGATGGACAATTACATGAGG
TGAAGTACGGTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTACATGATCGACAAAGAAATCGCTCGCGGAGAATTGGTGGAGTTAATGGCAGATAAAGTGTTGCCAGTGGCAATGCC
ATTCAGTGCAGTCTATTACAGCGACCGTGCGGTAAGTACGCGCATCCGGGCTTTTATCGATTTCCTTAGCGAGCATGTAA
AAACAGCTCCCGGAGGAGCTGTCAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTGTTGAGAACATTAAATCGGATGACATATCACGCATCCTGATGGTTTAATGACAAATAAATTCAAAATATCTCTTCT
ATTTTGCAACAGGAGCAAAA

Downstream 100 bases:

>100_bases
TCCCATTCAGGAGCCAGACCTTTCGGGCTAACCAGGCGGTCGTTGCAATCCAGGGCGGCGATCGCTTTTTTATCTTTGGC
ATCGAGCTGTAAATTTTGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=99.0131578947368, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.5640138408304, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=31.4878892733564, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.3783783783784, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=28.6206896551724, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.4722222222222, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=28.169014084507, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=26.1744966442953, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=24.3150684931507, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082132, Length=304, Percent_Identity=27.9605263157895, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=29.0909090909091, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=170, Percent_Identity=25.2941176470588, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789506, Length=311, Percent_Identity=22.8295819935691, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790399, Length=298, Percent_Identity=25.8389261744966, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33651; Mature: 33651

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]