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The map label for this gene is ligT

Identifier: 157159613

GI number: 157159613

Start: 160549

End: 161079

Strand: Reverse

Name: ligT

Synonym: EcHS_A0150

Alternate gene names: 157159613

Gene position: 161079-160549 (Counterclockwise)

Preceding gene: 157159616

Following gene: 157159612

Centisome position: 3.47

GC content: 55.56

Gene sequence:

>531_bases
ATGTCTGAACCGCAACGTCTGTTCTTTGCTATCGACTTACCTGCAGAAATCCGCGAACAGATTATCCATTGGCGCGCCAC
ACACTTCCCACCTGAGGCGGGACGTCCGGTCGCCGCCGATAATTTGCATCTGACTCTGGCATTTTTAGGCGAAGTGAGCG
CCGAGAAAGAGAAGGCGCTTTCTCTTTTAGCCGGACGGATTCGTCAACCTGGTTTCACACTCACGCTTGATGACGCCGGA
CAATGGCTGCGTTCGCGTGTGGTGTGGTTAGGGATGCGTCAGCCTCCACGCGGCTTAATCCAGCTGGCGAATATGCTCCG
TTCACAGGCTGCCCGCAGCGGTTGTTTTCAAAGCAATCGTCCGTTTCATCCACATATTACCTTATTGCGCGACGCCAGCG
AGGCGGTGACAATCCCGCCGCCAGGTTTTAACTGGTCTTATGCGGTGACGGAGTTCACCCTTTACGCCTCCTCGTTTGCC
CGTGGACGCACACGCTACACGCCGCTAAAACGCTGGGCGCTAACGCAATAA

Upstream 100 bases:

>100_bases
GACGCCAGCAACGGGCAACGACGACACAAAACGCTCCAGAGGGTTAACATTCTTCGCGCCACATTGTAGCATCGCGGTAA
TTCATAACCGAGTGCCTCAC

Downstream 100 bases:

>100_bases
CAAGGATTGTCGCAATGGAATTCTCTCCCCCTCTACAGCGCGCGACGCTAATTCAGCGTTACAAACGTTTTTTAGCCGAT
GTGATCACACCCGATGGTCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
RGRTRYTPLKRWALTQ

Sequences:

>Translated_176_residues
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
RGRTRYTPLKRWALTQ
>Mature_175_residues
SEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQ
WLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFAR
GRTRYTPLKRWALTQ

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=100, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIGT_ECOLI (P37025)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M60726
- PIR:   C64738
- RefSeq:   AP_000808.1
- RefSeq:   NP_414689.4
- ProteinModelPortal:   P37025
- SMR:   P37025
- STRING:   P37025
- EnsemblBacteria:   EBESCT00000003404
- EnsemblBacteria:   EBESCT00000017973
- GeneID:   944848
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5011
- KEGG:   eco:b0147
- EchoBASE:   EB2234
- EcoGene:   EG12330
- eggNOG:   COG1514
- GeneTree:   EBGT00050000010601
- HOGENOM:   HBG699408
- OMA:   FFALPCP
- ProtClustDB:   PRK15124
- BioCyc:   EcoCyc:EG12330-MONOMER
- BioCyc:   MetaCyc:EG12330-MONOMER
- Genevestigator:   P37025
- GO:   GO:0005622
- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097
- TIGRFAMs:   TIGR02258

Pfam domain/function: PF02834 2_5_RNA_ligase; SSF55144 Cyc_nuc_Pdiester

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19934; Mature: 19803

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112