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The map label for this gene is aroP [H]

Identifier: 157159580

GI number: 157159580

Start: 121817

End: 123187

Strand: Reverse

Name: aroP [H]

Synonym: EcHS_A0116

Alternate gene names: 157159580

Gene position: 123187-121817 (Counterclockwise)

Preceding gene: 157159585

Following gene: 157159577

Centisome position: 2.65

GC content: 52.74

Gene sequence:

>1371_bases
ATGGAAGGTCAACAGCACGGCGAGCAGCTAAAGCGCGGCCTTAAAAACCGCCATATTCAGCTTATCGCGCTGGGTGGCGC
GATAGGGACCGGGTTATTCCTGGGTAGCGCCTCCGTAATACAGTCCGCAGGGCCAGGGATTATCCTGGGTTACGCCATTG
CTGGTTTTATCGCCTTTCTGATCATGCGTCAGCTGGGTGAAATGGTGGTCGAAGAACCTGTCGCAGGCTCCTTTAGCCAC
TTTGCTTATAAATACTGGGGCAGTTTTGCCGGTTTCGCCTCTGGCTGGAACTACTGGGTACTGTACGTTTTAGTTGCCAT
GGCTGAGCTGACTGCCGTGGGTAAATACATTCAGTTCTGGTATCCGGAAATCCCCACCTGGGTTTCTGCCGCCGTATTCT
TTGTGGTGATTAACGCCATCAACCTGACCAACGTTAAAGTGTTTGGCGAGATGGAGTTCTGGTTTGCCATTATCAAAGTT
ATCGCGGTGGTAGCGATGATCATCTTCGGCGGCTGGCTGCTATTCAGTGGCAACGGCGGCCCGCAGGCGACCGTTAGCAA
CCTGTGGGATCAGGGTGGTTTCCTGCCGCACGGCTTCACCGGGCTGGTGATGATGATGGCGATTATCATGTTCTCGTTCG
GTGGTCTGGAACTGGTGGGGATCACCGCAGCAGAAGCTGATAACCCGGAGCAAAGTATACCGAAAGCAACTAACCAGGTT
ATCTACCGCATCCTGATTTTCTATATTGGTTCGTTAGCCGTTCTGCTCTCACTGATGCCGTGGACCCGCGTTACCGCCGA
TACCAGTCCGTTTGTGCTGATCTTCCACGAGTTAGGCGATACCTTTGTGGCGAATGCGCTGAACATCGTGGTACTGACTG
CGGCGCTCTCCGTGTACAACAGCTGCGTATATTGCAACAGCCGTATGCTGTTTGGTCTGGCACAACAGGGTAATGCGCCA
AAAGCGCTGGCGTCTGTCGATAAACGTGGTGTACCAGTAAATACCATTCTGGTGTCTGCACTGGTAACGGCGTTGTGCGT
ACTGATTAACTACCTTGCCCCAGAGTCCGCTTTCGGACTGTTAATGGCGCTGGTGGTATCTGCACTGGTAATCAACTGGG
CGATGATTAGCCTGGCGCATATGAAATTCCGTCGCGCCAAGCAGGAACAAGGCGTGGTAACTCGCTTCCCTGCTCTGCTT
TATCCGCTGGGTAACTGGATCTGCCTGCTGTTTATGGCGGCGGTACTGGTGATTATGCTGATGACCCCAGGAATGGCGAT
TTCGGTATACCTGATCCCGGTATGGCTGATCGTGTTAGGTATCGGCTATCTGTTTAAAGAGAAAACCGCCAAAGCCGTAA
AAGCGCATTAA

Upstream 100 bases:

>100_bases
CTTCTTTGATGTAAACAAATTAATACAACAAACGGAATTGCAAACTTACACACGCATCACTGCGTAGATCAAAAAAACAA
CCACCGCACGAGGTTTCATG

Downstream 100 bases:

>100_bases
TCTCTCTACGCCCTCACCCGTACAGGGTGAGGGCAATAATCTTTACACCAACGCCCCGTAAATCGTCAGTAGTGCAATCA
CCACCACGACCACGAACGAG

Product: aromatic amino acid transporter

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm]; Proton [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: General aromatic amino acid permease [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH

Sequences:

>Translated_456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
>Mature_456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of the aromatic amino acids (phenylalanine, tyrosine, and tryptophan) [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI258645169, Length=430, Percent_Identity=23.2558139534884, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI110347453, Length=356, Percent_Identity=23.876404494382, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI258614003, Length=431, Percent_Identity=22.737819025522, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI258614005, Length=431, Percent_Identity=22.737819025522, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI181337167, Length=292, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4507047, Length=388, Percent_Identity=21.6494845360825, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786302, Length=456, Percent_Identity=100, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=430, Percent_Identity=64.6511627906977, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790653, Length=461, Percent_Identity=40.3470715835141, Blast_Score=351, Evalue=6e-98,
Organism=Escherichia coli, GI1786602, Length=453, Percent_Identity=43.046357615894, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI48994972, Length=431, Percent_Identity=40.1392111368909, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87081915, Length=456, Percent_Identity=36.8421052631579, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI87081708, Length=465, Percent_Identity=35.0537634408602, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1789017, Length=433, Percent_Identity=36.7205542725173, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788480, Length=392, Percent_Identity=35.969387755102, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI87081869, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI2367353, Length=398, Percent_Identity=22.8643216080402, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17531343, Length=380, Percent_Identity=25.7894736842105, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532491, Length=398, Percent_Identity=27.1356783919598, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=372, Percent_Identity=23.3870967741935, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=449, Percent_Identity=35.8574610244989, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324061, Length=440, Percent_Identity=33.6363636363636, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324924, Length=458, Percent_Identity=33.4061135371179, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6324553, Length=398, Percent_Identity=33.9195979899498, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320717, Length=406, Percent_Identity=32.512315270936, Blast_Score=209, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6322892, Length=431, Percent_Identity=34.5707656612529, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6321053, Length=422, Percent_Identity=31.2796208530806, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319824, Length=402, Percent_Identity=31.592039800995, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=30.4250559284116, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324059, Length=440, Percent_Identity=33.1818181818182, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321629, Length=400, Percent_Identity=30.5, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319543, Length=398, Percent_Identity=30.9045226130653, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6320251, Length=405, Percent_Identity=29.6296296296296, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6319542, Length=406, Percent_Identity=28.8177339901478, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319608, Length=418, Percent_Identity=28.9473684210526, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6322967, Length=399, Percent_Identity=30.0751879699248, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324981, Length=417, Percent_Identity=28.7769784172662, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320364, Length=472, Percent_Identity=26.4830508474576, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24666159, Length=370, Percent_Identity=21.8918918918919, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221512776, Length=375, Percent_Identity=21.8666666666667, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24668806, Length=403, Percent_Identity=22.3325062034739, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356285, Length=403, Percent_Identity=22.3325062034739, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24668802, Length=403, Percent_Identity=22.3325062034739, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 49560; Mature: 49560

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFL
CCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHH
IMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
YPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNGG
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
PQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHHH
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHH
SCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGL
HHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAVLVIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFL
CCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHH
IMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
YPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNGG
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
PQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHHH
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHH
SCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGL
HHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAVLVIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm]; Proton [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + L-tyrosine [Periplasm] = Proton [Cytoplasm] + L-tyrosine [Cytoplasm] Proton [Periplasm] + L-phenylalanine [Periplasm] = Proton [Cytoplasm] +

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]