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The map label for this gene is setA [H]

Identifier: 157159540

GI number: 157159540

Start: 79376

End: 80554

Strand: Direct

Name: setA [H]

Synonym: EcHS_A0075

Alternate gene names: 157159540

Gene position: 79376-80554 (Clockwise)

Preceding gene: 157159534

Following gene: 157159546

Centisome position: 1.71

GC content: 48.35

Gene sequence:

>1179_bases
ATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGT
GGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGACTCT
TTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGA
AAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAATCGTCATTATCTGACGCTTAT
CACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATA
ACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACCGTTG
GCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATT
GATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGG
ATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTG
TGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGC
AATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGT
TTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATT
GTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAG
TATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGA
TAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACATTTGA

Upstream 100 bases:

>100_bases
TTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCG
CCGCGCTAAAAAGGGAACGT

Downstream 100 bases:

>100_bases
TGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCAT
AAGCGGCAATGGCGTCGTCG

Product: sugar efflux transporter A

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWIIAVISVVALFLTAKVKDI

Sequences:

>Translated_392_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWIIAVISVVALFLTAKVKDI
>Mature_392_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWIIAVISVVALFLTAKVKDI

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=392, Percent_Identity=98.7244897959184, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI1788495, Length=385, Percent_Identity=52.2077922077922, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1790091, Length=388, Percent_Identity=46.9072164948454, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42713; Mature: 42713

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCEEHHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTA
HHCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTVMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHH
FTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
HHHHHHHHHHHCCCHHEEECCCCCCEECCCCCCCCCEEEEEEHHHHHHHCCEEEEEECCH
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIL
HCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
FHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHHHHHHH
AIAQSWGHFAVYWIIAVISVVALFLTAKVKDI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNAI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCEEHHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRHYLTLITCGVLLASLANTA
HHCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTVMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHH
FTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPL
HHHHHHHHHHHCCCHHEEECCCCCCEECCCCCCCCCEEEEEEHHHHHHHCCEEEEEECCH
WISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIL
HCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
FHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHHHHHHH
AIAQSWGHFAVYWIIAVISVVALFLTAKVKDI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]