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The map label for this gene is rihC [H]

Identifier: 157159497

GI number: 157159497

Start: 30552

End: 31466

Strand: Direct

Name: rihC [H]

Synonym: EcHS_A0032

Alternate gene names: 157159497

Gene position: 30552-31466 (Clockwise)

Preceding gene: 157159496

Following gene: 157159498

Centisome position: 0.66

GC content: 56.28

Gene sequence:

>915_bases
ATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGA
ACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGC
ATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCGCTGGTACGCGCACCGCGTGATGCGGCATCTGTG
CACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGAT
TCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATTGGCCCGTTAACCAATATTGCGCTGTTACTTT
CACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGCCGGACGCGGCAACTGTACGCCA
AACGCCGAGTTTAATATCGCTGCCGATCCAGAAGCGGCTGCCTGTGTCTTCCGTAGCGGAATCGAAATCGTCATGTGCGG
TCTGGATGTCACCAATCAGGCAATATTAACCCCTGACTATCTCGCTACTTTGCCGGAGTTGAATCGCACCGGAAAAATGC
TTCATGCACTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGTCTGCGAATGCACGATCTCTGCGCCATCGCCTGG
CTGGTGCGCCCGGACTTATTCACCCTCAAGCCGTGTTTTGTCGCGGTAGAAACTCAAGGCGAATTTACCTCAGGCACGAC
GGTGGTTGATATCGACGGTTGCCTGGGCAAACCAGCCAATGTAAAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGC
AGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA

Upstream 100 bases:

>100_bases
AAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCG
GCATTTTTTATGGAGAAAAC

Downstream 100 bases:

>100_bases
CCTGTCACATGTTATCGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTC
TAATTATCAGCGTTTTTGGC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS

Sequences:

>Translated_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS
>Mature_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=304, Percent_Identity=99.0131578947368, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=44.9838187702265, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=40.0662251655629, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=33.5106382978723, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=32.1052631578947, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=342, Percent_Identity=28.9473684210526, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24641839, Length=196, Percent_Identity=32.6530612244898, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583915, Length=191, Percent_Identity=32.9842931937173, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641837, Length=328, Percent_Identity=30.4878048780488, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32546; Mature: 32546

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAS
HHCC
>Mature Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA