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The map label for this gene is yfbT [H]

Identifier: 157159313

GI number: 157159313

Start: 2581881

End: 2582531

Strand: Reverse

Name: yfbT [H]

Synonym: EcE24377A_2586

Alternate gene names: 157159313

Gene position: 2582531-2581881 (Counterclockwise)

Preceding gene: 157156942

Following gene: 157158626

Centisome position: 51.86

GC content: 56.22

Gene sequence:

>651_bases
GTGCGGTGCAAAGGTTTTCTGTTTGATCTTGATGGAACGCTGGTGGATTCCCTGCCTGCGGTAGAACGGGCGTGGAGCAA
CTGGGCCAGACGTCATGGGTTAGCGCCGGAAGAGGTGCTGGCTTTCATTCACGGTAAACAGGCGATCACCTCTTTGCGCC
ATTTTATGGCGGGCAAATCCGAGGCTGATATTGCCGCCGAGTTTACGCGTCTGGAGCACATCGAGGCCACGGAAACCGAA
GGTATTACCGCGCTTCCGGGGGCAATCGCCTTACTCAATCATTTGAATAAAGCAGGTATTCCGTGGGCCATTGTGACTTC
TGGCTCCATGCCGGTAGCGCGAGCGCGCCATAAAATAGCTGGGCTTCCCGCACCAGAGGTGTTTGTAACCGCTGAGCGAG
TGAAGCGCGGAAAACCAGAACCTGATGCGTATCTGTTAGGTGCGCAGCTGCTGGGGCTTGCGCCGCAGGAGTGTGTGGTG
GTGGAAGATGCTCCCGCTGGCGTGCTTTCTGGCCTGGCGGCGGGTTGCCATGTCATTGCGGTTAACGCTCCGGCAGATAC
CCCGCGCCTGAATGAGGTCGATTTGGTCCTCCACAGTCTGGAGCAAATTACTGTGACCAAACAGCCAAATGGCGATGTTA
TTATTCAGTGA

Upstream 100 bases:

>100_bases
AAATATCAGCGCATGCTTAATGTGTGGCATGCCTGCCCGCGTCAGTACCATTTGAGCGCCAACGAAATTAATCAAATTAT
CAATGCCTGAGGAGGCCCGC

Downstream 100 bases:

>100_bases
AACACCTGATCATGATTTAGCCCCGTCTCGTCGGGGCTTTTTTATGGCAGAATCAAGTCATCCCCCTCAATTAACAAGGA
TAAGTTGTGAACGGTGAATT

Product: putative phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Sequences:

>Translated_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
>Mature_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Homo sapiens, GI197382691, Length=212, Percent_Identity=30.188679245283, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI207113149, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87082080, Length=216, Percent_Identity=99.537037037037, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1790151, Length=202, Percent_Identity=30.6930693069307, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789046, Length=192, Percent_Identity=29.6875, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321834, Length=229, Percent_Identity=32.3144104803493, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321833, Length=229, Percent_Identity=31.8777292576419, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322136, Length=225, Percent_Identity=31.5555555555556, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320905, Length=225, Percent_Identity=31.5555555555556, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129151, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23035; Mature: 23035

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC
>Mature Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]