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The map label for this gene is tusC

Identifier: 157159165

GI number: 157159165

Start: 3789680

End: 3790039

Strand: Reverse

Name: tusC

Synonym: EcE24377A_3813

Alternate gene names: 157159165

Gene position: 3790039-3789680 (Counterclockwise)

Preceding gene: 157158490

Following gene: 157159145

Centisome position: 76.11

GC content: 49.44

Gene sequence:

>360_bases
ATGAAACGAATTGCGTTTGTTTTTTCTACTGCACCTCATGGTACAGCTGCTGGTCGGGAAGGTTTAGATGCTTTACTGGC
AACTTCCGCATTAACTGACGATCTGACCGTCTTCTTTATTGCTGATGGCGTTTTTCAGCTGCTGTCAGGACAAAAGCCCG
ATGCAGTGCTGGCGCGTGATTACATTGCCACTTTTAAATTGTTGGGTCTGTACGACATTGAACAGTGCTGGGTTTGTGCG
GCTTCACTGCGCGAACGCGGGTTAGATCCGCAGACACCCTTTGTTGTCGAAGCCACGCCGCTCGAAGCAGATGCCTTACG
CCGCGAACTCGCCAACTACGATGTTATTTTGAGGTTTTGA

Upstream 100 bases:

>100_bases
TGGGGCTGGCTTCGTCAAACCTTCAGCCGGGATTTACCTTAAGCGGACTTGGCGCGCTGGCGGAAGCCTCGCTGACCTGT
GACAGGGTGGTACAGTTCTG

Downstream 100 bases:

>100_bases
GGCGTTTATGCTGCACACGTTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAG
ACGAACTGCTATTATTGCAA

Product: sulfur relay protein TusC

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein C

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGVFQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCA
ASLRERGLDPQTPFVVEATPLEADALRRELANYDVILRF

Sequences:

>Translated_119_residues
MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGVFQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCA
ASLRERGLDPQTPFVVEATPLEADALRRELANYDVILRF
>Mature_119_residues
MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGVFQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCA
ASLRERGLDPQTPFVVEATPLEADALRRELANYDVILRF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2923

COG function: function code P; Uncharacterized protein involved in the oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrF/tusC family

Homologues:

Organism=Escherichia coli, GI1789742, Length=119, Percent_Identity=98.3193277310924, Blast_Score=236, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSC_ECO24 (A7ZSL9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464798.1
- ProteinModelPortal:   A7ZSL9
- SMR:   A7ZSL9
- STRING:   A7ZSL9
- EnsemblBacteria:   EBESCT00000018973
- GeneID:   5590744
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_3813
- NMPDR:   fig|331111.3.peg.1475
- eggNOG:   COG2923
- GeneTree:   EBGT00050000010996
- HOGENOM:   HBG496129
- OMA:   FKVLPLY
- ProtClustDB:   PRK00211
- BioCyc:   ECOL331111:ECE24377A_3813-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00389
- InterPro:   IPR003787
- InterPro:   IPR017462
- TIGRFAMs:   TIGR03010

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 13065; Mature: 13065

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGVFQLLSGQKPDAVLARD
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHH
YIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLEADALRRELANYDVILRF
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGVFQLLSGQKPDAVLARD
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHH
YIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLEADALRRELANYDVILRF
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA