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The map label for this gene is ygfT [H]

Identifier: 157159085

GI number: 157159085

Start: 3232474

End: 3234393

Strand: Reverse

Name: ygfT [H]

Synonym: EcE24377A_3212

Alternate gene names: 157159085

Gene position: 3234393-3232474 (Counterclockwise)

Preceding gene: 157155962

Following gene: 157154983

Centisome position: 64.95

GC content: 52.92

Gene sequence:

>1920_bases
ATGAATAAGTTTATCGCTGCTGAAGCTGCGGAATGTATAGGCTGTCATGCTTGTGAAATTGCCTGTGCGGTGGCACACAA
TCAAGAAAACTGGCCGCTGAGTCACAGTGACTTTCGACCGCGTATCCACGTTGTAGGGAAAGGCCAGGCTGCGAATCCGG
TGGCCTGCCATCACTGCAACAATGCCCCTTGCGTTACGGCTTGTCCGGTTAATGCTCTGACTTTCCAGTCCGATAGCGTA
CAACTGGACGAGCAAAAATGTATTGGCTGTAAAAGATGCGCAATCGCTTGCCCCTTTGGCGTCGTTGAGATGGTCGATAC
GATTGCGCAGAAATGCGACCTTTGTAACCAGCGCAGTTCCGGCACGCAAGCCTGTATTGAAGTCTGCCCAACACAGGCGT
TACGACTGATGGACGATAAAGGGTTACAGCAGATAAAGGTGGCCCGCCAGCGCAAAACGGCAGCAGGAAAAGCGTCATCA
GACGCTCAGCCATCTCGCAGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAGATAAAATTTCAGCGAGTGAACG
GAAAACCCACTTTGGCGAAATATATTGCGGGCTGGATCCACAACAAGCGACTTATGAGAGTGACCGCTGTGTTTATTGTG
CCGAAAAAGCTAACTGCAACTGGCATTGCCCGCTGCATAACGCTATTCCGGATTACATCCGTCTGGTACAGGAAGGAAAG
ATTATTGAAGCGGCAGAACTTTGCCACCAGACCAGTTCCTTACCAGAAATTTGCGGCAGGGTATGTCCACAGGACCGTCT
TTGTGAAGGCGCATGTACTTTGAAAGATCACTCGGGCGCAGTCTCTATCGGTAATCTGGAACGCTACATCACCGATACCG
CGCTGGCGATGGGCTGGCGTCCCGATGTCAGCAAAGTTGTTCCCCGTATCGAAAAAGTGGCGGTGATTGGCGCTGGGCCA
GCAGGGTTAGGGTGTGCTGATATTCTGGCGCGCGCGGGAGTTCAGGTTGATGTCTTTGATCGCCATCCAGAAATTGGCGG
TATGCTGACTTTTGGCATTCCTCCTTTCAAACTCGATAAAACGGTATTAAGCCAGCGGCGAGAGATATTCACCGCAATGG
GAATCGACTTTCATCTTAACTGTGAAATTGGCCGCGATATTACCTTTAGCGATTTAACTTCTGAATATGATGCAGTTTTC
ATCGGCGTGGGGACTTACGGGATGATGCGAGCAGATCTGCCGCATGAAGATGCGCCCGGTGTCATTCAGGCTCTACCGTT
CCTGACTGCCCATACCCGCCAGCTCATGGGATTGCCGGAGTCTGAAGAGTATCCGCTGACGGACGTGGAAGGTAAGCGAG
TCGTGGTATTGGGCGGTGGCGATACGACAATGGATTGTTTGCGGACTTCCATCCGCCTCAATGCCGCCAGCGTGACCTGC
GCGTATCGTCGTGATGAAGTCAGTATGCCGGGCTCGCGCAAAGAGGTGGTTAATGCGCGCGAGGAAGGTGTCGAGTTTCA
GTTCAATGTTCAACCGCAATATATCGCTTGTGACGAAGATGGGCGTTTAACTGCGGTGGGCCTGATTCGTACCGCCATGG
GTGAGCCGGGGCCGGATGGTCGCCGTCGTCCTCGTCCGGTAGCGGGTTCAGAGTTTGAATTGCCCGCCGATGTTCTGATT
ATGGCCTTTGGTTTCCAGGCGCATGCCATGCCGTGGTTGCAGGGCAGCGGAATTAAACTCGATAAATGGGGCCTGATTCA
AACCGGTGACGTCGGGTATTTACCTACCCAGACGCATCTGAAAAAAGTCTTTGCTGGTGGTGATGCAGTTCATGGCGCGG
ATCTGGTTGTCACTGCAATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTGTTTGATACGAAGGCATCGTGA

Upstream 100 bases:

>100_bases
CGCAAAATTATAATTAAAACGGTCATGCTACCTAATATATGCCCGTTTATTGCATCGCGAAATTTGAGAAGACATAACTC
ATTAAATGAAAGGGATGCAA

Downstream 100 bases:

>100_bases
TGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTAGCCTGTGTGGTCGCTCACCCT
TCAGAACAGGAGTTGAATGC

Product: putative oxidoreductase Fe-S binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSV
QLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQEGK
IIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGP
AGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVF
IGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASVTC
AYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLI
MAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS

Sequences:

>Translated_639_residues
MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSV
QLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQEGK
IIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGP
AGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVF
IGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASVTC
AYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLI
MAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
>Mature_639_residues
MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSV
QLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQEGK
IIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGP
AGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVF
IGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASVTC
AYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLI
MAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=472, Percent_Identity=29.4491525423729, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI111118981, Length=162, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI111118983, Length=162, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=639, Percent_Identity=99.8435054773083, Blast_Score=1320, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=656, Percent_Identity=54.5731707317073, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=59.5555555555556, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=28.7305122494432, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=33.6448598130841, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=46.0122699386503, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=44.8717948717949, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=39.3063583815029, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=34.3915343915344, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87082114, Length=203, Percent_Identity=33.4975369458128, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI2367345, Length=167, Percent_Identity=32.3353293413174, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789370, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1787122, Length=153, Percent_Identity=28.7581699346405, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1787872, Length=151, Percent_Identity=29.8013245033113, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=30.9576837416481, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71984108, Length=521, Percent_Identity=29.5585412667946, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17543860, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=31.8872017353579, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=33.1958762886598, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=33.1958762886598, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI28574881, Length=475, Percent_Identity=33.6842105263158, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24665539, Length=475, Percent_Identity=33.6842105263158, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24640763, Length=465, Percent_Identity=27.9569892473118, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI18858217, Length=465, Percent_Identity=27.9569892473118, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00037 Fer4; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 69116; Mature: 69116

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCN
CCCCCHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCC
NAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSS
CCCEEEECCCCEEEECCCCCEECHHHCCCHHHHEEECCHHHHHHHHHHHHHHHHHCCCCC
GTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNSRKGAD
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
KISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQEGK
CCCCHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCC
IIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWR
EEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHCCCC
PDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDK
CCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHHHH
TVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPHEDAPG
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCCCC
VIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASVTC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECEEEEE
AYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDG
EEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCCCCCCC
RRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHL
CCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCCCHHHH
KKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCN
CCCCCHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCC
NAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSS
CCCEEEECCCCEEEECCCCCEECHHHCCCHHHHEEECCHHHHHHHHHHHHHHHHHCCCCC
GTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNSRKGAD
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
KISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQEGK
CCCCHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCC
IIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWR
EEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHCCCC
PDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDK
CCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHHHH
TVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPHEDAPG
HHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCCCC
VIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASVTC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECEEEEE
AYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDG
EEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCCCCCCC
RRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHL
CCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCCCHHHH
KKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]