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The map label for this gene is narY [H]

Identifier: 157159065

GI number: 157159065

Start: 1640747

End: 1642291

Strand: Reverse

Name: narY [H]

Synonym: EcE24377A_1648

Alternate gene names: 157159065

Gene position: 1642291-1640747 (Counterclockwise)

Preceding gene: 157155119

Following gene: 157156278

Centisome position: 32.98

GC content: 54.17

Gene sequence:

>1545_bases
ATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAA
AAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGCATTGGTTATCCGA
AAAACTGGGAAGATCAGGAAGAGTGGCAAGGCGGCTGGGTCCGCGATGTGAATGGCAAGATCCGTCCGCGTCTGGGCAGC
AAGATGGGCGTAATAACCAAAATCTTCGCCAACCCGGTGGTGCCGCAGATTGATGATTACTACGAACCTTTCACTTTCGA
CTACGAACATTTGCATAGCGCACCGGAAGGCAAACATATCCCTACTGCTCGCCCGCGCTCGCTGATTGACGGTAAGCGGA
TGGACAAAGTGATCTGGGGGCCAAACTGGGAAGAACTGCTGGGCGGCGAGTTTGAAAAACGTGCCCGCGACCGCAACTTC
GAGGCCATACAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
CAATCCCAGTTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGG
ATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCA
GAAAAATGTATCTTCTGTTATCCGCGAATTGAGTCAGGCCAACCCACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCG
GTATCTGGGCGTGCTGCTTTACGACGCCGACCGCATCGAGGAAGCGGCGAGCACCGAGCGCGAAGTTGACCTCTATGAAC
GTCAGTGCGAAGTGTTCCTCGATCCACACGATCCCTCAGTGATCGAGGAAGCCCTGAAACAAGGTATTCCACAAAACGTG
ATTGAAGCTGCCCAGCGTTCGCCTGTCTACAAAATGGCGATGGACTGGAAACTGGCGCTACCGCTGCACCCTGAATATCG
CACCCTGCCAATGGTCTGGTACGTTCCTCCGCTGTCGCCGATTCAGTCCTACGCAGATGCGGGCGGTTTGCCGAAAAGCG
AAGGCGTGCTGCCCGCCATCGAAAGCCTACGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGT
CCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAGGCGTTACTGATAC
TCGTGCCATCGACGAAGTAGGCCTGAGCGTCGCCCAGGTCGAAGAAATGTATCGCTATCTCGCCATTGCCAACTATGAAG
ATCGTTTTGTTATCCCGACGAGCCATCGGGAAATGGCGGGCGATGCCTTCGCAGAACGAAACGGCTGCGGTTTTACCTTT
GGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATCAACATCACCGAAGT
GCGCGACAAAGCGGAGGGCGAATAA

Upstream 100 bases:

>100_bases
ACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGA
TCAGGTACAGGAGGCGAAAA

Downstream 100 bases:

>100_bases
TGCAGATCCTCAAAGTGATCGGCCTGTTGATGGAGTATCCGGACGAGCTGTTGTGGGAATGCAAGGAGGACGCGCTGGCG
TTGATCCGCCGCGACGCGCC

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=99.4163424124514, Blast_Score=1071, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=79.2490118577075, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=41.5841584158416, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58527; Mature: 58527

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]