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The map label for this gene is gntR [H]

Identifier: 157159015

GI number: 157159015

Start: 3895571

End: 3896566

Strand: Reverse

Name: gntR [H]

Synonym: EcE24377A_3916

Alternate gene names: 157159015

Gene position: 3896566-3895571 (Counterclockwise)

Preceding gene: 157158486

Following gene: 157159305

Centisome position: 78.25

GC content: 53.92

Gene sequence:

>996_bases
ATGAAAAAGAAAAGACCCGTACTTCAGGATGTGGCTGACCGTGTAGGCGTGACCAAAATGACGGTCAGCCGCTTTTTGCG
TAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCGTG
CGCCCGATATCCTCTCTAACGCCACCAGCCGGGCGATTGGCGTCCTGTTACCTTCTCTCACCAACCAGGTTTTCGCGGAA
GTATTACGCGGAATCGAAAGCGTCACCGACGCGCACGGTTATCAAACCATGCTGGCGCACTACGGTTATAAACCGGAAAT
GGAGCAAGAACGCCTCGAATCCATGCTCTCATGGAATATCGATGGACTGATCCTCACCGAACGTACTCACACGCCACGCA
CCTTAAAGATGATTGAAGTGGCGGGGATCCCGGTGGTGGAGTTGATGGACAGCAAGTCGCCGTGCCTCGATATTGCCGTC
GGTTTTGATAACTTTGAAGCGGCACGCCAGATGACCACCGCCATTATTGCTCGCGGGCATCGCCACATTGCTTATCTCGG
CGCACGTCTCGACGAACGTACTATCATCAAACAGAAGGGATACGAACAGGCGATGCTGGATGCAGACCTGGTGCCATATA
GCGTGATGGTTGAGCAATCTTCTTCTTACTCTTCCGGTATTGAACTGATTCGCCAGGCGAGGCGGGAATATCCGCAGCTG
GATGGCGTGTTCTGTACTAATGATGATCTGGCGGTCGGCGCGGCGTTTGAATGTCAGCGTCTGGGGTTAAAAGTTCCTGA
CGATATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTGGCGAGCGTGCTGACGCCGC
GTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTATTCGTGGCGAATCTGTGACACCGAAAATGTTAGAT
TTAGGTTTCACCTTGTCACCGGGCGGATCTATTTAA

Upstream 100 bases:

>100_bases
GTATATCTAATAATTGTAAACAAGCACTGTTTTCCCCGGGAAGGTTCTGATGCGTCCGTGTTAAACTAAGAGAATCTATC
TCTTTTGTACCTTCAGGACG

Downstream 100 bases:

>100_bases
GCCCACAAATTTGAAGTAGCTCACACTTATACACTTAAGGCACGGATGGATATTGCTTCTGATATTGTCCGGCCGGACAA
TGTTACCGATAACAGTTACC

Product: transcriptional regulator GntR

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Sequences:

>Translated_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI
>Mature_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=99.6978851963746, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=46.0843373493976, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1790369, Length=323, Percent_Identity=29.1021671826625, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=28.3536585365854, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=27.5862068965517, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=27.5757575757576, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=26.0586319218241, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=25.5520504731861, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=24.9211356466877, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786540, Length=325, Percent_Identity=26.1538461538462, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=22.5255972696246, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=23.2628398791541, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36481; Mature: 36481

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC
>Mature Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]