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The map label for this gene is leuS [H]

Identifier: 157158979

GI number: 157158979

Start: 694877

End: 697459

Strand: Reverse

Name: leuS [H]

Synonym: EcE24377A_0668

Alternate gene names: 157158979

Gene position: 697459-694877 (Counterclockwise)

Preceding gene: 157156509

Following gene: 157158850

Centisome position: 14.01

GC content: 53.16

Gene sequence:

>2583_bases
ATGCAAGAGCAATACCGCCCGGAAGAGATAGAATCCAAAGTACAGCTTCACTGGGATGAGAAGCGCACATTTGAAGTAAC
CGAAGACGAGAGCAAAGAGAAGTATTACTGCCTGTCTATGCTTCCCTATCCTTCTGGTCGACTACACATGGGCCACGTAC
GTAACTACACCATCGGTGACGTGATCGCCCGCTACCAGCGTATGCTGGGCAAAAACGTCCTGCAGCCGATCGGCTGGGAC
GCGTTTGGTCTGCCTGCGGAAGGCGCGGCGGTGAAAAACAACACCGCACCGGCACCGTGGACGTACGACAACATCGCGTA
CATGAAAAACCAGCTCAAAATGCTGGGCTTTGGTTATGACTGGAGCCGCGAGCTGGCAACCTGTACGCCGGAATACTACC
GTTGGGAACAGAAATTCTTCACCGAGCTGTATAAAAAAGGCCTGGTATATAAGAAGACTTCTGCGGTCAACTGGTGCCCG
AACGACCAGACCGTACTGGCGAACGAACAAGTTATCGACGGCTGCTGCTGGCGCTGCGATACCAAAGTTGAACGTAAAGA
GATCCCGCAGTGGTTTATCAAAATCACCGCTTACGCTGACGAGCTGCTCAACGATCTGGATAAACTGGATCACTGGCCAG
ATACCGTTAAAACCATGCAGCGTAACTGGATCGGTCGTTCCGAAGGCGTAGAAATCACCTTCAACGTTAACGACTATGAC
AACACGCTGACCGTTTACACTACCCGCCCGGACACCTTTATGGGTTGTACCTACCTGGCGGTAGCGGCGGGTCATCCGCT
GGCGCAGAAAGCGGCGGAAAATAATCCTGAACTGGCCGCCTTTATTGACGAATGCCGTAACACCAAAGTTGCCGAAGCTG
AAATGGCGACGATGGAGAAAAAAGGCGTCGATACTGGCTTTAAAGCGGTTCATCCATTAACGGGCGAAGAAATTCCGGTT
TGGGCAGCAAACTTCGTATTGATGGAGTACGGCACGGGCGCAGTTATGGCGGTTCCGGGTCACGACCAGCGCGACTACGA
GTTTGCCTCTAAATACGGCCTGAACATCAAACCGGTTATCCTGGCAGCTGACGGCTCTGAGCCAGATCTTTCTCAACAAG
CCCTGACTGAAAAAGGCGTGCTGTTCAACTCTGGCGAGTTCAACGGTCTTGACCATGAAGCGGCCTTCAACGCCATCGCC
GATAAACTGACTGCGATGGGCGTTGGCGAGCGTAAAGTGAACTACCGCCTGCGCGACTGGGGTGTTTCTCGTCAGCGTTA
CTGGGGTGCGCCGATTCCGATGGTTACGCTGGAAGACGGTACCGTAATGCCGACCCCGGACGACCAGCTGCCGGTGATTC
TGCCGGAAGATGTGGTGATGGACGGCATTACCAGCCCGATTAAAGCAGATCCGGAGTGGGCAAAAACTACCGTTAACGGT
ATTCCAGCGCTGCGTGAAACCGACACTTTCGACACCTTTATGGAGTCCTCCTGGTACTATGCGCGCTACACTTGCCCGGA
GTACAAAGAAGGTATGCTGGATTCTAAAGCGGCTAACTACTGGCTGCCGGTGGATATCTACATTGGTGGTATCGAACACG
CCATTATGCACCTGCTCTACTTCCGCTTCTTCCACAAACTGATGCGTGATGCAGGCATGGTGAACTCTGACGAACCAGCG
AAACAGTTGCTGTGTCAGGGTATGGTGCTGGCAGATGCCTTCTACTATGTTGGCGAAAACGGCGAACGTAACTGGGTTTC
CCCGGTTGATGCTATCGTTGAACGTGACGAGAAAGGCCGTATCGTGAAAGCGAAAGATGCGGCAGGCCATGAACTGGTTT
ATACCGGCATGAGCAAAATGTCCAAGTCGAAGAACAACGGTATCGACCCGCAGGTGATGGTTGAACGTTACGGCGCGGAC
ACCGTTCGTCTGTTTATGATGTTTGCTTCTCCGGCTGATATGACTCTCGAATGGCAGGAATCCGGCGTGGAAGGGGCTAA
CCGCTTCCTGAAACGTGTCTGGAAACTGGTTTACGAGCACACAGCAAAAGGTGATGTTGCGGCACTGAACGTTGATGCGC
TGACTGAAGATCAGAAAGCGCTGCGTCGCGATGTGCATAAAACTATCGCTAAAGTGACCGATGATATCGGCCGTCGTCAG
ACCTTCAACACCGCAATTGCGGCGATTATGGAGCTGATGAACAAACTGGCGAAAGCACCAACCGATGGCGAGCAGGATCG
CGCTCTGATGCAGGAAGCGCTGCTGGCCGTTGTCCGTATGCTTAACCCGTTCACCCCGCACATCTGCTTCACGCTGTGGC
AGGAACTGAAAGGCGAAGGCGATATCGACAACGCGCCGTGGCCGGTTGCTGACGAAAAAGCGATGGTGGAAGACTCCACG
CTGGTCGTGGTGCAGGTTAACGGTAAAGTCCGTGGCAAAATCACCGTTCCGGTGGACGCAACGGAAGAACAGGTTCGCGA
ACGTGCTGGCCAGGAACATCTGGTAGCAAAATATCTTGATGGCGTTACTGTACGTAAAGTGATTTACGTACCAGGTAAAC
TCCTCAATCTGGTCGTTGGCTAA

Upstream 100 bases:

>100_bases
GGCTGTTTTGACGCGGACGTTTGGGCTATGCTATGCGGATCTGAAAAACCACCTCAACGCTACATTTGTAGCCGTATTGA
AAACAGGACCACTGGCTGCC

Downstream 100 bases:

>100_bases
GCGCGGGAGGAAGCGTGCGATATCTGGCAACATTGTTGTTATCTCTGGCGGTGTTAATCACCGCCGGGTGTGGCTGGCAT
CTGCGTGATACCACGCAGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
IPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Sequences:

>Translated_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
IPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG
>Mature_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
IPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=865, Percent_Identity=36.4161849710983, Blast_Score=513, Evalue=1e-145,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=99.4186046511628, Blast_Score=1786, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=398, Percent_Identity=26.6331658291457, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71997510, Length=885, Percent_Identity=30.0564971751412, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71997517, Length=877, Percent_Identity=30.1026225769669, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI212645227, Length=441, Percent_Identity=26.984126984127, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554638, Length=86, Percent_Identity=38.3720930232558, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71980946, Length=151, Percent_Identity=28.476821192053, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=809, Percent_Identity=37.4536464771323, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6321531, Length=387, Percent_Identity=26.6149870801034, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325217, Length=357, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355409, Length=882, Percent_Identity=33.1065759637188, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281366294, Length=471, Percent_Identity=22.7176220806794, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 97204; Mature: 97204

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHEECCCC
WSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCEEEE
LAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDW
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
IPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEEEECCCCCCEEEEHH
SGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEKAMVEDSTLVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC
>Mature Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHEECCCC
WSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCEEEE
LAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDW
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
IPALRETDTFDTFMESSWYYARYTCPEYKEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEEEECCCCCCEEEEHH
SGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEKAMVEDSTLVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA