Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 157158594

GI number: 157158594

Start: 1921378

End: 1923765

Strand: Reverse

Name: pheT [H]

Synonym: EcE24377A_1932

Alternate gene names: 157158594

Gene position: 1923765-1921378 (Counterclockwise)

Preceding gene: 157158955

Following gene: 157159273

Centisome position: 38.63

GC content: 53.85

Gene sequence:

>2388_bases
ATGAAATTCAGTGAATTGTGGTTACGCGAATGGGTGAACCCGGCGATTGATAGCGATGCGCTGGCGAATCAAATCACTAT
GGCGGGCCTGGAAGTTGACGGTGTAGAACCGGTTGCCGGTAGCTTCCACGGCGTGGTCGTTGGTGAAGTGGTTGAGTGTG
CGCAGCATCCGAACGCTGACAAACTGCGTGTGACAAAAGTGAATGTTGGCGGCGATCGCCTGCTGGACATCGTCTGCGGT
GCGCCAAACTGCCGTCAGGGCCTGCGTGTGGCGGTAGCTACCATTGGTGCTGTTCTGCCGGGTGATTTCAAAATTAAAGC
GGCGAAACTGCGTGGTGAACCGTCTGAAGGGATGCTGTGCTCCTTTTCTGAGCTGGGAATTTCTGACGATCATAGCGGCA
TTATCGAGCTGCCTGCGGATGCGCCGATTGGCACCGACATCCGTGAATACCTGAAACTTGATGACAACACCATCGAAATC
AGCGTGACGCCAAACCGTGCCGACTGTTTAGGTATCATTGGTGTTGCGCGTGACGTTGCCGTGCTGAATCAGCTGCCGCT
GGTTGAACCGGAAATCGTTCCGGTTGGTGCGACCATCGACGACACGCTGCCGATTACAGTCGAAGCGCCGGAAGCCTGCC
CGCGTTATCTTGGCCGTGTGGTAAAAGGCATTAACGTTAAAGCGCCAACTCCGCTGTGGATGAAAGAAAAACTGCGTCGT
TGCGGGATCCGTTCTATCGATGCAGTTGTTGACGTCACCAACTATGTACTGCTCGAACTGGGCCAGCCGATGCACGCTTT
CGATAAAGATCGCATTGAAGGCGGCATTGTGGTGCGGATGGCGAAAGAGGGCGAAACGTTGGTACTGCTTGATGGCACTG
AAGCGAAGCTGAATGCTGACACTCTGGTCATCGCCGACCACAACAAGGCGCTGGCGATGGGCGGCATCTTTGGTGGCGAA
CACTCTGGCGTGAATGACGAAACGCAAAACGTCTTGCTGGAATGCGCTTTCTTCAGCCCGCTGTCTATCACCGGTCGTGC
TCGTCGTCATGGCCTGCATACTGATGCGTCTCACCGTTATGAGCGTGGCGTTGATCCGGCACTGCAGCACAAAGCGATGG
AACGTGCGACCCGTCTGCTGATCGACATCTGCGGTGGTGAGGCTGGTCCGGTAATTGATATCACCAACGAAGCAACGCTG
CCGAAGCGTGCAACCATCACTTTACGTCGTAGCAAACTGGATCGCCTGATCGGCCATCATATTGCGGATGAGCAGGTAAC
TGACATTCTGCGTCGTCTCGGCTGCGAAGTGACCGAAGGCAAAGACGAGTGGCAGGCAGTTGCGCCGAGCTGGCGTTTCG
ATATGGAGATTGAAGAAGATCTGGTCGAAGAAGTCGCGCGTGTTTACGGCTACAACAACATCCCGGATGAGCCGGTACAG
GCAAGCCTGATTATGGGTACTCACCGTGAAGCTGACCTGTCGCTCAAGCGCGTGAAAACGCTGCTCAACGACAAAGGCTA
TCAGGAAGTGATCACCTACAGCTTCGTTGATCCGAAAGTGCAGCAGATGATCCATCCAGGCGTTGAAGCCTTACTGCTGC
CAAGCCCGATCTCTGTTGAAATGTCAGCAATGCGTCTTTCTCTGTGGACCGGCCTGCTGGCAACCGTGGTGTACAACCAG
AACCGTCAGCAGAACCGTGTGCGCATTTTCGAAAGCGGTCTGCGTTTCGTACCAGATACTCAAGCACCGTTGGGCATTCG
TCAGGATCTGATGTTAGCCGGTGTGATTTGCGGTAACCGTTACGAAGAGCACTGGAACCTGGCAAAAGAGACCGTTGATT
TCTATGATTTGAAAGGCGATCTTGAATCCGTTCTCGACCTGACCGGTAAACTGAATGAGGTTGAGTTCCGTGCAGAAGCG
AATCCGGCACTGCATCCGGGGCAATCCGCAGCGATTTATCTGAAAGGTGAACGTATTGGTTTTGTTGGGGTTGTTCATCC
TGAACTGGAACGTAAACTGGATCTTAACGGTCGCACTCTGGTGTTCGAACTGGAGTGGAACAAGCTCGCAGACCGCGTGG
TGCCTCAGGCGCGCGAGATTTCTCGCTTCCCGGCGAACCGTCGTGACATCGCGGTGGTGGTCGCAGAAAACGTTCCCGCA
GCGGATATTTTATCCGAATGTAAGAAAGTTGGCGTAAATCAGGTAGTTGGCGTAAACTTATTTGACGTGTACCGCGGTAA
GGGTGTTGCGGAGGGGTATAAGAGCCTCGCCATAAGCCTGATCCTGCAAGATACCAGCCGTACACTCGAAGAAGAGGAGA
TTGCCGCTACCGTCGCCAAATGTGTAGAGGCATTAAAAGAGCGATTCCAGGCATCATTGAGGGATTGA

Upstream 100 bases:

>100_bases
AGCGTCTGACTATGTTGCGTTACGGCGTCACCGACCTGCGTTCATTCTTCGAAAATGATCTGCGTTTCCTCAAACAGTTT
AAATAAGGCAGGAATAGATT

Downstream 100 bases:

>100_bases
ACCTATGGCGCTTACAAAAGCTGAAATGTCAGAATATCTGTTTGATAAGCTTGGGCTTAGCAAGCGGGATGCCAAAGAAC
TGGTTGAACTGTTTTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=99.874213836478, Blast_Score=1618, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=388, Percent_Identity=22.9381443298969, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87380; Mature: 87380

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNAD
CCHHHHHHHHHCCCCCCCHHHHHHHEEECEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVTPNRADCLGIIGVARDVA
EHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
HHHCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRY
EEEEEECCCEEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCHHHHCCCCCCCHHHH
ERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
IADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
CCHHHHHHHHHHHCCEECCCCHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVE
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCCEEEECCCCCCH
MSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
YEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEE
FVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCH
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAK
HHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHEEHEEEECHHHHHHHHHHHHHHHH
CVEALKERFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNAD
CCHHHHHHHHHCCCCCCCHHHHHHHEEECEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVTPNRADCLGIIGVARDVA
EHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
HHHCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRY
EEEEEECCCEEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCHHHHCCCCCCCHHHH
ERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
IADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
CCHHHHHHHHHHHCCEECCCCHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVE
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCCEEEECCCCCCH
MSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
YEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEE
FVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCH
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAK
HHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHEEHEEEECHHHHHHHHHHHHHHHH
CVEALKERFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]