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The map label for this gene is pphA [H]

Identifier: 157158545

GI number: 157158545

Start: 2049910

End: 2050566

Strand: Reverse

Name: pphA [H]

Synonym: EcE24377A_2067

Alternate gene names: 157158545

Gene position: 2050566-2049910 (Counterclockwise)

Preceding gene: 157157533

Following gene: 157158805

Centisome position: 41.18

GC content: 47.79

Gene sequence:

>657_bases
ATGAAACAGCCTGCGCCCGTTTATCAGAGAATTGCGGGTCATCAATGGCGACATATCTGGCTTTCTGGCGATATACACGG
TTGTCTTGAGCAGTTGCGCCGCAAATTATGGCATTGTGGTTTTGATCCGTGGCGAGATTTACTTATCTCAGTGGGAGACG
TTATCGATCGTGGGCCGCAAAGTTTACGTTGCCTGCAGTTACTGGAACAACATTGGGTTCGTGCGGTAAGAGGCAATCAT
GAACAGATGGCGATGGATGCGCTGGCATCCCAGCAGATGTCTTTGTGGTTGATGAATGGCGGCGACTGGTTTATTGCGCT
GGCAGATAATCAACAGAAACAAGCGAAAACGGCGCTGGAAAAATGTCAGCATTTGCCCTTTATTCTTGAAGTACACAGTC
GTACCGGCAAACATGTTATTGCTCATGCCGATTATCCAGATGATGTTTATGAATGGCAAAAGGACGTTGATTTGCATCAG
GTCTTGTGGAGCCGCTCGCGATTAGGTGAACGCCAAAAAGGGCAGGGAATTACAGGTGCTGATCATTTCTGGTTTGGTCA
TACACCGTTGCGACATCGCGTGGATATTGGCAACCTGCATTATATTGATACCGGTGCTGTCTTTGGGGGCGAACTGACTC
TTGTGCAATTGCAATAA

Upstream 100 bases:

>100_bases
CTTTATAGTCAACCATAACACACACTCTACTGTCTGAGTCCAGCGTTTTTTAACATTCTTGTTAAGATTATGTGATCTTT
AGCGCGGGAGGAAAATATTG

Downstream 100 bases:

>100_bases
TTAAAAATCACCATACTCCTGTGCTGGTCGCCAGAAACCATCTATAAAATCCTCAATCGGAAAACAACCGCCATGGCGGA
TCCGTTGATCGCTCATAGAA

Product: serine/threonine protein phosphatase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ

Sequences:

>Translated_218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
>Mature_218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=218, Percent_Identity=99.0825688073395, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789090, Length=217, Percent_Identity=43.778801843318, Blast_Score=188, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25228; Mature: 25228

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPWRDLLISVGDVIDRGPQ
CCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCHHHHHHHHH
KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITGA
HHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
CCCEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC
>Mature Secondary Structure
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPWRDLLISVGDVIDRGPQ
CCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCHHHHHHHHH
KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITGA
HHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
CCCEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]