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The map label for this gene is uidA

Identifier: 157158494

GI number: 157158494

Start: 1820086

End: 1821897

Strand: Reverse

Name: uidA

Synonym: EcE24377A_1825

Alternate gene names: 157158494

Gene position: 1821897-1820086 (Counterclockwise)

Preceding gene: 157156031

Following gene: 157156112

Centisome position: 36.59

GC content: 52.26

Gene sequence:

>1812_bases
ATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAA
CTGTGGAATTGATCAGCGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAACGATC
AGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCA
GGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCA
TCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTG
TGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAAACGGCAAGAAAAAGCAGTCTTAC
TTCCATGATTTCTTTAACTATGCCGGGATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATAT
CACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTCAGCG
TTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGGACTTTGCAAGTGGTGAATCCGCAC
CTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGTGCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCT
TCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTG
GTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGG
ATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGT
GGTGATTGATGAAACTGCTGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAAC
TGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAA
AACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCC
ACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCG
ATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACG
GCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGT
GGATACGTTAGCCGGGCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC
ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATA
TTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACG
CTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGA

Upstream 100 bases:

>100_bases
CTTCCATGGGTTTCTCACAGATAACTGTGTGCAACACAGAATTGGTTAACTAATCAGATTAAAGGTTGACCAGTATTTTT
ATCTTAATGAGGAGTCCCTT

Downstream 100 bases:

>100_bases
ATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGTGACGTCGCCAATAACTTCGCCTTCGCAATGGGGGCG
CTCTTCCTATTGAGTTACTA

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Sequences:

>Translated_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ
>Mature_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family

Homologues:

Organism=Homo sapiens, GI268834192, Length=636, Percent_Identity=44.9685534591195, Blast_Score=505, Evalue=1e-143,
Organism=Escherichia coli, GI1787903, Length=603, Percent_Identity=100, Blast_Score=1264, Evalue=0.0,
Organism=Escherichia coli, GI1786539, Length=484, Percent_Identity=26.6528925619835, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI48994920, Length=392, Percent_Identity=25.5102040816327, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=617, Percent_Identity=36.4667747163695, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI62471735, Length=624, Percent_Identity=43.2692307692308, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19922582, Length=624, Percent_Identity=43.2692307692308, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24655438, Length=624, Percent_Identity=43.2692307692308, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24651745, Length=633, Percent_Identity=39.652448657188, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BGLR_ECOLI (P05804)

Other databases:

- EMBL:   M14641
- EMBL:   S69414
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64918
- RefSeq:   AP_002238.1
- RefSeq:   NP_416134.1
- ProteinModelPortal:   P05804
- SMR:   P05804
- DIP:   DIP-11086N
- IntAct:   P05804
- STRING:   P05804
- PRIDE:   P05804
- EnsemblBacteria:   EBESCT00000003130
- EnsemblBacteria:   EBESCT00000016677
- GeneID:   946149
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1609
- KEGG:   eco:b1617
- EchoBASE:   EB1048
- EcoGene:   EG11055
- eggNOG:   COG3250
- GeneTree:   EBGT00050000009399
- HOGENOM:   HBG474923
- OMA:   IADVFIN
- ProtClustDB:   PRK10150
- BioCyc:   EcoCyc:BETA-GLUCURONID-MONOMER
- BioCyc:   MetaCyc:BETA-GLUCURONID-MONOMER
- Genevestigator:   P05804
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:2.60.40.320
- Gene3D:   G3DSA:3.20.20.80
- PRINTS:   PR00132

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N; SSF49785 Gal_bind_like; SSF49303 Glyco_hydro_2Ig; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.31

Molecular weight: Translated: 68448; Mature: 68448

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: ACT_SITE 413-413

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHCCCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC
>Mature Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHCCCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503