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The map label for this gene is fadI [H]

Identifier: 157158369

GI number: 157158369

Start: 2629682

End: 2630992

Strand: Reverse

Name: fadI [H]

Synonym: EcE24377A_2638

Alternate gene names: 157158369

Gene position: 2630992-2629682 (Counterclockwise)

Preceding gene: 157158947

Following gene: 157155802

Centisome position: 52.84

GC content: 56.06

Gene sequence:

>1311_bases
ATGGGTCAGGTTTTACCGCTGGTTACCCGCCAGGGCGATCGTATCGCCATTGTTAGCGGTTTACGTACGCCTTTTGCCCG
CCAGGCGACGGCTTTTCATGGCATTCCCGCGGTTGATTTAGGGAAGATGGTGGTAGGCGAACTGCTGGCACGCAGCGAGA
TCCCCGCTGAAGTGATTGAACAGCTGGTCTTTGGTCAGGTCGTACAAATGCCTGAAGCCCCCAACATTGCGCGTGAAATT
GTTCTCGGTACCGGAATGAATGTGCATACCGATGCTTACAGCGTCAGCCGCGCTTGCGCTACCAGTTTCCAGGCGGTTGC
AAACGTCGCAGAAAGCCTGATGGCGGGAACTATCCGAGCGGGGATTGCCGGTGGGGCAGATTCCTCTTCCGTATTGCCAA
TTGGCGTCAGTAAAAAACTGGCGCGCGTGCTGGTTGATGTCAACAAAGCTCGAACCATGAGCCAGCGACTGAAACTCTTC
TCTCGCCTGCGTTTGCGCGACTTAATGCCCGTACCGCCTGCGGTAGCAGAATATTCTACTGGCTTGCGGATGGGCGACAC
CGCCGAACAAATGGCGAAAACCTACGGCATCACCCGAGAACAGCAAGATGCATTAGCGCACCGTTCGCATCAGCGTGCCG
CTCAGGCGTGGTCAGACGGTAAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCTCCTTATAAACAACCGCTTGTCGAA
GACAACAATATTCGCGGTAATTCCTCGCTTGCCGATTACGCAAAGCTGCGCCCGGCGTTTGATCGCAAACACGGAACGGT
AACGGCGGCGAACAGTACGCCGCTGACCGATGGCGCAGCGGCGGTGATCCTGATGACTGAATCCCGGGCGAAAGAATTAG
GGCTGGTGCCGCTGGGGTATCTGCGCAGCTACGCATTTACTGCGATTGATGTCTGGCAGGACATGTTGCTCGGTCCGGCC
TGGTCAACACCGCTGGCGCTGGAACGTGCCGGTTTGACAATGAGCGATCTGACATTGATCGATATGCACGAAGCCTTTGC
AGCTCAGACACTGGCGAATATTCAGTTGCTGGGTAGTGAACGTTTTGCTCGTGAAGTACTGGGGCGTGCACATGCCACTG
GCGAAGTGGACGATAGCAAATTTAACGTGCTTGGCGGTTCGATTGCTTACGGGCATCCCTTCGCGGCGACCGGCGCGCGG
ATGATTACCCAGACATTGCATGAACTTCGCCGTCGCGGCGGTGGATTTGGTTTAGTTACCGCCTGTGCTGCCGGTGGGCT
TGGCGCGGCAATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
GCTCCCACTTTCCTTTTTCTCCGCTTGGCTAAATGTAAAAAAATGGTTAAGACTATGATCAGGTCAGACCACTTTATTTA
TTTTTTTACAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TGGAAATGGCATCAGTGTTTACTCTTAATGTTCGTCTGGACAACATTGCTATCATCACCATCGACGTACCGGGTGAGAAA
ATGAATACCCTGAAGGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_435_residues
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIV
LGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFS
RLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVED
NNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAW
STPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARM
ITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=41.8052256532066, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=29.7872340425532, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=32.5581395348837, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=30.4245283018868, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=29.9065420560748, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=39.622641509434, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=99.7706422018349, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.6973995271868, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.7014218009479, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI48994986, Length=437, Percent_Identity=35.2402745995423, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=33.0985915492958, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=38.7706855791962, Blast_Score=300, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.2206572769953, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.5714285714286, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=29.7169811320755, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=420, Percent_Identity=24.2857142857143, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325229, Length=438, Percent_Identity=30.5936073059361, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=440, Percent_Identity=28.1818181818182, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=41.1627906976744, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.3838862559242, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.6995305164319, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17648125, Length=430, Percent_Identity=29.3023255813954, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46559; Mature: 46428

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCHH
RFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCHH
RFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA