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The map label for this gene is yafV [H]

Identifier: 157158346

GI number: 157158346

Start: 272738

End: 273508

Strand: Reverse

Name: yafV [H]

Synonym: EcE24377A_0251

Alternate gene names: 157158346

Gene position: 273508-272738 (Counterclockwise)

Preceding gene: 157159278

Following gene: 157158244

Centisome position: 5.49

GC content: 54.99

Gene sequence:

>771_bases
GTGCCTGGTTTGAAGATAACGCTTTTGCAGCAACCACTGGTGTGGATGGATGGCCCTGCCAACCTGCGTCATTTTGATCG
TCAACTGGAAGGTATTACCGGGCGCGATGTGATCGTTCTACCGGAGATGTTTACCAGCGGCTTTGCCATGGAAGCGGCAG
CTTCGTCGCTAGCACAAGATGACGTAGTGAACTGGATGACAGCTAAGGCGCAGCAGTGCAATGCGCTGATTGCAGGCAGT
GTTGCATTACAAACGGAGTCTGGTTCGGTTAACCGCTTTTTGCTGGTTGAGCCGGGCGGCACGGTACATTTTTATGATAA
GCGTCATCTGTTCCGCATGGCAGATGAGCATCTACATTATAAAGCGGGCAATGCGCGAGTGATTGTGGAATGGCGCGGCT
GGCGTATTTTGCCGTTGGTGTGCTACGACTTACGTTTTCCGGTGTGGTCGCGCAATCTCAACGATTATGACCTCGCCATC
TACGTTGCCAACTGGCCTGCTCCGCGCTCTCTGCACTGGCAGGCATTGCTGACGGCCCGCGCGATTGAGAATCAGGCGTA
TGTGGCGGGATGCAATCGCGTCGGCAGCGATGGCAACGGCTGCCATTATCGCGGTGACAGCCGGGTGATTAATCCACAAG
GCGAGATTATCGCTACTGCCGACGCGCATCAGGCAACGCGCATTGATGCCGAGCTGTCGATGGCGGTGTTAAGGGAATAT
CGCGATAAGTTTCCGGCATGGCGGGATGCGGATGAGTTTAGGTTGCGGTGA

Upstream 100 bases:

>100_bases
TTCCAATATCAGCATTTGTTATCAATTTATTCTGCGCTAAGGCAACTGCCTGATAAGGTGTTAAAGTTATAAAACCCTTC
GCATAAAGGAGTTGTACACC

Downstream 100 bases:

>100_bases
GTGGGGGTTGTGTTTAAGGCTGGGGGAGAGTCGGGGTAAGACTACGAAAGCCCGCTCTCCGCAAGGACTGACGCCAGATA
GTTTCTGTCCATAGCTGCAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGS
VALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAVLREY
RDKFPAWRDADEFRLR

Sequences:

>Translated_256_residues
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGS
VALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAVLREY
RDKFPAWRDADEFRLR
>Mature_255_residues
PGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSV
ALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAIY
VANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAVLREYR
DKFPAWRDADEFRLR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786412, Length=255, Percent_Identity=98.4313725490196, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28937; Mature: 28806

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQD
CCCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCCCEEEECHHHCCCCHHHHHHHHHHHH
DVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHY
HHHHHHHHHHHHCCEEEECEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHEEE
KAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAIYVANWPAPRSLHWQALLTAR
ECCCEEEEEEECCCEEEEEEEEEECCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAVLREY
HHCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCEEEECCCCCCEECCHHHHHHHHHHH
RDKFPAWRDADEFRLR
HHHCCCCCCCHHHCCC
>Mature Secondary Structure 
PGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAASSLAQD
CCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCCCEEEECHHHCCCCHHHHHHHHHHHH
DVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHY
HHHHHHHHHHHHCCEEEECEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHEEE
KAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAIYVANWPAPRSLHWQALLTAR
ECCCEEEEEEECCCEEEEEEEEEECCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMAVLREY
HHCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCEEEECCCCCCEECCHHHHHHHHHHH
RDKFPAWRDADEFRLR
HHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]