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The map label for this gene is menD [H]

Identifier: 157158309

GI number: 157158309

Start: 2552446

End: 2554116

Strand: Reverse

Name: menD [H]

Synonym: EcE24377A_2560

Alternate gene names: 157158309

Gene position: 2554116-2552446 (Counterclockwise)

Preceding gene: 157157025

Following gene: 157159079

Centisome position: 51.29

GC content: 57.99

Gene sequence:

>1671_bases
ATGTCAGTAAGCGCATTTAACCGACGCTGGGCGGCGGTCATTCTGGAAGCATTAACGCGTCACGGCGTCAGACACATCTG
TATCGCCCCAGGCTCGCGTTCTACACCGTTAACGTTAGCGGCGGCGGAGAATTCCGCATTCATTCACCACACCCATTTCG
ATGAGCGTGGACTGGGGCATCTGGCGCTGGGGCTGGCGAAAGTCAGCAAGCAGCCGGTGGCGGTGATTGTGACCTCCGGC
ACGGCGGTGGCAAATCTCTATCCGGCACTGATTGAAGCCGGGTTAACCGGAGAAAAACTGATTCTCTTAACCGCCGATCG
CCCGCCGGAGCTAATTGACTGCGGCGCGAATCAGGCAATTCGCCAGCCGGGAATGTTCGCCTCTCACCCCACGCACAGTA
TTTCATTGCCGCGCCCGACCCAGGATATCCCCGCATGTTGGCTGGTTTCTACCATCGACCACGCTCTCGGTACGCTTCAT
GCGGGGGGAGTCCATATCAACTGCCCGTTTGCTGAACCGCTGTATGGCGAAATGGACGATACCGGGCTTAGCTGGCAACA
GCGTCTGGGTGACTGGTGGCAGGACGACAAACCGTGGCTGCGTGAAGCGCCTCGTCTGGAAAGTGAAAAACAGCGCGACT
GGTTCTTCTGGCGACAAAAGCGCGGCGTGGTGGTTGCCGGGCGCATGAGTGCGGAAGAGGGCAAAAAAGTTGCCCTGTGG
GCGCAAACTCTTGGCTGGCCGCTGATTGGCGATGTGCTGTCACAAACCGGGCAGCCGCTGCCGTGTGCCGATCTTTGGTT
AGGCAATGCCAAAGCGACCAGCGAGCTGCAGCAGGCGCAAATTGTGGTGCAACTGGGAAGCAGCCTGACGGGGAAACGGC
TCCTGCAATGGCAGGCAAGCTGTGAACCCGAAGAGTACTGGATTGTTGATGACATTGAAGGGCGACTTGATCCGGCACAC
CATCGCGGACGTCGCTTAATTGCCAATATTGCCGACTGGCTGGAGCTGCATCCGGCAGAAAAACGCCAGCCCTGGTGCGT
TGAAATCCCGCGCCTGGCGGAACAGGCAATGCAGGCGGTTATTGCCCGCCGTGATGCGTTTGGCGAAGCGCAACTGGCGC
ATCGCATCAGCGACTATCTGCCTGAACAGGGGCAATTGTTTGTTGGTAACAGCCTGGTGGTACGTCTGATTGATGCGCTT
TCGCAACTTCCGGCAGGTTACCCGGTGTACAGCAACCGAGGGGCCAGCGGTATCGACGGACTGCTTTCGACCGCCGCAGG
CGTTCAGCGGGCAAGCGGCAAACCGACGCTGGCGATTGTGGGCGATCTCTCCGCACTTTACGATCTCAACGCGCTGGCGT
TATTGCGTCAGGTTTCTGCGCCGCTGGTATTAATTGTGGTGAACAACAACGGCGGGCAAATTTTCTCGCTGTTGCCAACG
CCGCAAAGCGAGCGTGAGCGTTTCTATCTGATGCCGCAAAACGTCCATTTTGAGCACGCCGCCGCGATGTTCGAGCTGAA
ATATCATCGTCCGCAAAACTGGCAGGAACTTGAAACGGCATTTGCCGACGCCTGGCGCACGCCAACCACCACGGTGATTG
AAATGGTGGTTAACGACACCGATGGTGCGCAAACGCTCCAGCAACTTCTGGCGCAGGTAAGCCATTTATGA

Upstream 100 bases:

>100_bases
CAGATGGAATAGTAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAA
TATTGATACCGGACAAACTC

Downstream 100 bases:

>100_bases
TCCTGCACGCGCAGGCAAAACACGGAAAACCAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTGCCAC
GAATGGCAAGAAGTGGGCGA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG
TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLH
AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH
HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDAL
SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL

Sequences:

>Translated_556_residues
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG
TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLH
AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH
HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDAL
SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL
>Mature_555_residues
SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGT
AVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLHA
GGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALWA
QTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHH
RGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALS
QLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPTP
QSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=556, Percent_Identity=99.6402877697842, Blast_Score=1128, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 61299; Mature: 61167

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH
LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC
TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
CCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCEEEEE
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQAS
HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
IARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG
HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDT
CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEECCC
DGAQTLQQLLAQVSHL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH
LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC
TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
CCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCEEEEE
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQAS
HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
IARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG
HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDT
CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEECCC
DGAQTLQQLLAQVSHL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA