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The map label for this gene is rluE [H]

Identifier: 157158295

GI number: 157158295

Start: 1300170

End: 1300823

Strand: Reverse

Name: rluE [H]

Synonym: EcE24377A_1298

Alternate gene names: 157158295

Gene position: 1300823-1300170 (Counterclockwise)

Preceding gene: 157157286

Following gene: 157156203

Centisome position: 26.12

GC content: 51.22

Gene sequence:

>654_bases
ATGCGGCAATTCATAATCTCTGAAAATACCATGCAAAAAACTTCTTTTAGAAATCACCAGGTTAAGCGATTCAGCTCGCA
ACGTTCTACCAGGCGTAAACCTGAAAACCAGCCCACGCGTGTGATCCTGTTCAATAAACCCTACGATGTTCTTCCGCAGT
TCACCGATGAAGCCGGACGCAAAACATTAAAAGAATTCATCCCGGTTCAGGGCGTTTATGCAGCAGGTCGTCTTGACCGC
GATAGCGAAGGGTTACTGGTGCTGACCAATAACGGCGCGTTGCAGGCGCGTTTAACCCAGCCGGGCAAACGCACCGGGAA
AATCTATTATGTGCAGGTGGAAGGTATTCCCACACAAGACGCACTTGAAGCCTTGCGCAATGGCGTAACCTTAAATGATG
GCCCTACCCTGCCCGCAGGCGCGGAGCTGGTTGACGAACCCGCGTGGTTATGGCCGCGGAATCCACCAATTCGTGAACGC
AAAAGTATTCCCACCAGCTGGCTGAAGATCACCTTATATGAAGGACGTAATCGCCAGGTGCGCCGCATGACCGCCCATGT
TGGTTTCCCCACGCTGCGACTGATTCGCTATGCGATGGGAGATTACTCTTTGGATAATCTTGCCAATGGCGAATGGCGAG
AAGTGACAGATTAA

Upstream 100 bases:

>100_bases
ACGGATGCTTTAGAGCAATTTTTTGTTAATGATTTGTAATTGGCTTGTCAATACTACCCTATTGTTTGCCGTCATGAAAG
ATGCGCGTTATTAGGCTATA

Downstream 100 bases:

>100_bases
GGATGCAAAATGTTTAAACCGCACGTTACCGTTGCTTGCGTGGTGCACGCAGAAGGCAAATTTTTAGTCGTTGAAGAGAC
GATTAATGGTAAAGCGTTAT

Product: 23S rRNA pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD

Sequences:

>Translated_217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD
>Mature_217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=217, Percent_Identity=100, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI1787524, Length=193, Percent_Identity=33.6787564766839, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790453, Length=184, Percent_Identity=29.8913043478261, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=32.967032967033, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24881; Mature: 24881

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGR
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHH
KTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQD
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHH
ALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQV
HHHHHHCCEEECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD
HHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCEEECCC
>Mature Secondary Structure
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGR
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHH
KTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQD
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHH
ALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQV
HHHHHHCCEEECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD
HHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]