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The map label for this gene is chbR [H]

Identifier: 157158276

GI number: 157158276

Start: 1944227

End: 1945066

Strand: Reverse

Name: chbR [H]

Synonym: EcE24377A_1956

Alternate gene names: 157158276

Gene position: 1945066-1944227 (Counterclockwise)

Preceding gene: 157157611

Following gene: 157156065

Centisome position: 39.06

GC content: 41.55

Gene sequence:

>840_bases
ATGCAGCCAGTGATTAACGCGCCGGAAATTGCCACTGCCCGAGAACAGCAGTTGTTTAATGGCAAAAACTTCCATGTGTT
TATCTATAACAAAACTGAGAGTATCAGCGGGCTGCATCAGCACGACTATTATGAATTTACTCTGGTATTAACCGGGCGTT
ATTTCCAGGAGATTAACGGTAAGCGCGTGTTACTGGAACGGGGCGATTTTGTTTTTATTCCGTTAGGTTCGCACCATCAA
AGTTTTTATGAGTTTGGTGCCATGCGTATATTGAACGTTGGGATCAGTAAACGCTTTTTTGAGCAGCATTACCTGCCGTT
GTTGCCTTATTGCTTTGTCGCTTCGCAGGTATACCGGACCAATAACGCTTTTCTCACCTATGTGGAAACAGTGATTTCTT
CATTGAATTTCCGCGAAACAGGGCTGGAAGAGTTTGTTGAGATGGTTACTTTTTATGTCATTAACCGTTTACGTCATTAC
CGCGAAGAACAGGTGATTGATGATGTACCGCAGTGGCTGAAAAGTACGGTAGAAAAGATGCATGATAAAGAGCAGTTTAG
TGAATCGGCGCTGGAGAATATGGTGACGTTGTCAGCCAAATCACAGGAATATTTGACGCGAGCGACTCAACGATATTATG
GCAAAACACCAATGCAGATTATTAATGAGATCCGTATTAATTTTGCCAAAAAACAACTGGAAATGACCAACTATTCCGTG
ACGGATATTGCGTTTGAGGCCGGTTATAGTAGCCCGAGTTTGTTTATTAAAACGTTTAAGAAATTAACGTCCTTTACGCC
TAAGAGCTATCGTAAGAAATTGACTGAATTTAATCAGTAA

Upstream 100 bases:

>100_bases
GTCCACGCTCAGGATCATTTAATGACGTCCATGCTTGCGCGTGAACTGATTACTGAATTAATTGAGCTTCATGAAAAACT
GAAGGCATAAGGAGTCGATG

Downstream 100 bases:

>100_bases
GTCGTTATACCTGACAATTCACATATCTGTCCTGTTGCTGGATTATTTATGTCCGGGGGGGCAGATATGCCAGATATCAG
TATTCTGTACTGAAGGGAGA

Product: DNA-binding transcriptional regulator ChbR

Products: NA

Alternate protein names: Chb operon repressor [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQ
SFYEFGAMRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSV
TDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ

Sequences:

>Translated_279_residues
MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQ
SFYEFGAMRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSV
TDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
>Mature_279_residues
MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQ
SFYEFGAMRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSV
TDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ

Specific function: Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presen

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788030, Length=279, Percent_Identity=99.2831541218638, Blast_Score=574, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32897; Mature: 32897

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEING
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHCCCEEEEEEEEHHHHHHCCC
KRVLLERGDFVFIPLGSHHQSFYEFGAMRILNVGISKRFFEQHYLPLLPYCFVASQVYRT
CEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQWLKSTVEKM
CCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCH
TDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEING
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHCCCEEEEEEEEHHHHHHCCC
KRVLLERGDFVFIPLGSHHQSFYEFGAMRILNVGISKRFFEQHYLPLLPYCFVASQVYRT
CEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQWLKSTVEKM
CCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCH
TDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 9405618 [H]