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The map label for this gene is yagT [H]

Identifier: 157158200

GI number: 157158200

Start: 325405

End: 326094

Strand: Reverse

Name: yagT [H]

Synonym: EcE24377A_0297

Alternate gene names: 157158200

Gene position: 326094-325405 (Counterclockwise)

Preceding gene: 157158349

Following gene: 157156073

Centisome position: 6.55

GC content: 55.07

Gene sequence:

>690_bases
ATGAGCAACCAAGGCGAATACCCCGAAGATAATCGGGTTGGGAAGCACGAGCCGCACGATTTTAGTTTGACCCGTCGCGA
TCTGATTAAAGTGAGCGCCGCAACGGCGGCGACCGCCGTGGTTTATCCTCATTCTACGCTTGCGGCAAGCGTTCCGGCAG
CTACACCCGCGCCAGAGATAATGCCCCTGACACTTAAGGTGAACGGCAAAACCGAGCAGCTTGAGGTGGATACCCGAACC
ACGCTACTGGACGCTTTGCGTGAAAATCTGCATCTGATCGGTACCAAGAAAGGTTGCGATCACGGACAGTGCGGAGCCTG
TACCGTGCTGGTCAATGGTCGCAGGCTTAATGCCTGCCTGACGCTGGCAGTCATGCATCAGGGGGCCGAGATCACCACCA
TTGAAGGCCTGGGTTCACCAGATAATCTTCACCCCATGCAGGCGGCCTTTATCAAGCATGATGGCTTCCAGTGCGGCTAC
TGCACCTCCGGGCAAATTTGCTCATCAGTAGCGGTGCTAAAAGAGATTCAGGACGGCATTCCCAGTCACGTCACGGTCGA
TTTGGTTTCCCCTCCAGAAACAACTGCCGATGAGATCCGTGAACGTATGAGCGGCAACATCTGTCGCTGTGGTGCATACG
CTAACATCCTTGCCGCCATTGAAGACGCTGCGGGGGAGATAAAATCATGA

Upstream 100 bases:

>100_bases
TAATCCTCCTGCTGTCGCCGACTATGCTTAACGTTTAAAAAAGCATCAGCACTCTCGCAACGCACTCTTATTTTCCCCTT
TAGAATACCGGAGGCCTGGT

Downstream 100 bases:

>100_bases
AGGCGTTTACCTATGAACGAGTGAACACCCCAGCCGAGGCGGCACTTAGCGCTCAGCGCGTACCCGGCGCAAAATTTATC
GCGGGCGGGACCAATCTGCT

Product: putative xanthine dehydrogenase iron-sulfur-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRT
TLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS

Sequences:

>Translated_229_residues
MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRT
TLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
>Mature_228_residues
SNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTT
LLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYC
TSGQICSSVAVLKEIQDGIPSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS

Specific function: Iron-sulfur subunit of the xanthine dehydrogenase complex [H]

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=175, Percent_Identity=38.8571428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI71773480, Length=169, Percent_Identity=37.8698224852071, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1786480, Length=229, Percent_Identity=98.6899563318777, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789232, Length=164, Percent_Identity=35.9756097560976, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540638, Length=181, Percent_Identity=36.4640883977901, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32566215, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17539860, Length=169, Percent_Identity=33.1360946745562, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17737937, Length=177, Percent_Identity=37.8531073446328, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647197, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24647195, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24647199, Length=149, Percent_Identity=33.5570469798658, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647193, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647201, Length=172, Percent_Identity=31.3953488372093, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2 [H]

EC number: NA

Molecular weight: Translated: 24373; Mature: 24242

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCCCCE
MPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACL
EEEEEEECCCCEEEEEHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCHHHHE
TLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGI
EEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC
PSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
CCCEEEEEECCCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCCCCE
MPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACL
EEEEEEECCCCEEEEEHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCHHHHE
TLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGI
EEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC
PSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS
CCCEEEEEECCCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]