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The map label for this gene is dsbB [H]

Identifier: 157158035

GI number: 157158035

Start: 1323181

End: 1323711

Strand: Reverse

Name: dsbB [H]

Synonym: EcE24377A_1330

Alternate gene names: 157158035

Gene position: 1323711-1323181 (Counterclockwise)

Preceding gene: 157158871

Following gene: 157156225

Centisome position: 26.58

GC content: 52.17

Gene sequence:

>531_bases
ATGTTGCGATTTTTGAACCAATGTTCACAAGGCCGGGGCGCGTGGCTGTTGATGGCGTTTACTGCTCTGGCACTGGAACT
GACGGCGCTGTGGTTCCAGCATGTGATGTTACTGAAACCTTGCATGCTCTGTATTTATGAACGTTGTGCGTTATTCGGCG
TTCTGGGTGCTGCGCTGATTGGCGCGATCGCCCCGAAAACTCCGCTGCGTTATGTAGCGATGGTTATCTGGTTGTATAGT
GCGTTCCGCGGTGTGCAGTTAACTTACGAGCACACCATGCTTCAGCTCTATCCTTCGCCGTTTGCCACCTGTGATTTTAT
GGTTCGTTTCCCGGAATGGCTGCCGCTGGATAAATGGGTGCCGCAAGTGTTTGTCGCCTCTGGCGATTGTGCCGAGCGTC
AGTGGGATTTTTTAGGTCTGGAAATGCCGCAGTGGCTGCTCGGTATTTTTATCGCTTACCTGATTGTCGCGGTGCTGGTG
GTGATTTCCCAGCCGTTTAAAGCGAAAAAACGTGACCTGTTCGGTCGCTAA

Upstream 100 bases:

>100_bases
GATAATTGTCCAATTGCTTCCTATTTTTGCTGCCTCCTGGTGGCGCAGCGAATGAATTGGTTTAAACTGCGCACTCTATG
CATATTGCAGGGAAATGATT

Downstream 100 bases:

>100_bases
TCCCTTCAGCGCTCCTGCGGGAGCGCTTTTTTCCTGCCGCTATATTTATTTGACCCGCAGTAAATCAGAACTTCGCGTTG
TATAACGTGGTGAAAGCATG

Product: disulfide bond formation protein B

Products: NA

Alternate protein names: Disulfide oxidoreductase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCMLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR

Sequences:

>Translated_176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCMLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR
>Mature_176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCMLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein [H]

COG id: COG1495

COG function: function code O; Disulfide bond formation protein DsbB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DsbB family [H]

Homologues:

Organism=Escherichia coli, GI1787433, Length=176, Percent_Identity=99.4318181818182, Blast_Score=356, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003752
- InterPro:   IPR022920 [H]

Pfam domain/function: PF02600 DsbB [H]

EC number: NA

Molecular weight: Translated: 20175; Mature: 20175

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCMLCIYERCALFGVLGAALI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHH
PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCMLCIYERCALFGVLGAALI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHH
PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]