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The map label for this gene is aglA [H]

Identifier: 157157824

GI number: 157157824

Start: 4178748

End: 4180625

Strand: Reverse

Name: aglA [H]

Synonym: EcE24377A_4190

Alternate gene names: 157157824

Gene position: 4180625-4178748 (Counterclockwise)

Preceding gene: 157159179

Following gene: 157159014

Centisome position: 83.95

GC content: 51.44

Gene sequence:

>1878_bases
ATGCGCCCACAAGTTCTGCTTAATCGATTGAAAAAACATCTTTTTTTTAAAGATGTGTTCGATGCTGTGTACCTGTGCTC
ACAGATGTCTACTTTTTCGCGAAAACGTAGATCTCTACCGCCCAACGAAAAGCATGAAAGCGATCACGAATCCCATTGGG
TCGTCATGTTCTGCCGATCGCATCTTCCTATCCTCGCTCCAGGCCTGCCGCATAACCAATCAGGCTTCCTACTTACAGAA
TTGAGAAAAGAGGATGTGGAAATGCTCAGTCAAATTCAACGCTTTGGCGGCGCGATGTTCACGCCAGTGCTGCTGTTTCC
CTTCGCCGGGATTGTGGTGGGTCTTGCCATCTTGCTGCAAAACCCGATGTTTGTCGGGGAATCACTGACCGATCCGAACA
GTTTATTCGCGCAAATCGTACACATTATTGAAGAGGGCGGTTGGACGGTGTTCCGTAATATGCCGCTGATTTTTGCTGTC
GGTTTACCCATTGGCCTTGCTAAGCAAGCGCAGGGGCGTGCTTGTCTGGCGGTGATGGTGAGTTTCCTGACCTGGAACTA
TTTCATCAACGCGATGGGAATGACCTGGGGAAGCTACTTCGGCGTCGATTTCACTCAGGACGCGGTGGCAGGTAGCGGTC
TGACAATGATGGCCGGGATTAAAACCCTCGATACCAGCATTATCGGCGCAATTATCATTTCCGGCATTGTGACGGCGCTG
CATAACCGTCTGTTCGATAAAAAACTGCCGGTTTTTCTCGGCATTTTCCAGGGGACGTCTTATGTGGTGATTATCGCCTT
CCTGGTGATGATCCCCTGTGCCTGGCTGACGTTGCTCGGCTGGCCAAAAGTACAAATGGGGATTGAATCTCTGCAAGCGT
TCCTGCGTTCGGCGGGTGCGCTTGGGGTGTGGGTTTACACCTTCCTCGAACGTATTCTGATCCCAACCGGTTTACACCAC
TTCATCTACGGACCGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGA
GTTCAGTCTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTG
GTGCCGTGGGCATTTCTTTAGCGATGTACTTCACTGCCGCACCGGAAAATCGGGTAAAAGTGGCGGGCTTGCTGATTCCC
GCAACCTTAACCGCCATGCTGGTGGGAATTACCGAACCGCTGGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGC
GGTACACGCTGTGCTTGCGGCCTCAATGTCGACCGTGATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGA
TTGACCAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCATTGGG
TTGTGCTTTACCCTGCTGTACTTCGTGGTTTTCCGCACACTGATTCTGCAGTTCAACATGTGCACGCCGGGACGTGAAGA
TGCGGAAGTGAAACTCTACTCAAAAGCCGAATACAAAGCCTCGCGAGGCCAAACCACCGCGGCAGAGCCAAAAAAAGAGC
TGGATCAGGCTGCCGGTATCCTGCAAGCCCTGGGCGGGGTCGGCAATATCTCCAGCATTAACAATTGCGCGACGCGTTTA
CGTATTGCACTGCATGACATGTCACAAACGCTGGATGACGAAGTCTTTAAAAAGCTGGGAGCGCACGGCGTCTTCCGTAG
TGGCGATGCCATTCAGGTGATCATTGGTCTGCATGTATCCCAGCTGCGTGAACAGCTCGATAGCTTAATTAATTCTCATC
AATCAGCAGAAAATGTTGCCATTACGGAGGCAGTATAA

Upstream 100 bases:

>100_bases
ATTTAGCGTGAAATCTGCGGAATTAAGTCGAATTCTTAACCGCTCCGCAATGCTTTTGTAGATCATCTCTAGATCTCTTT
TTTGCCTGCAAGCACGGGTA

Downstream 100 bases:

>100_bases
TGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAG
GACCGTTTCCCGCTTCGTGC

Product: PTS system, alpha-glucoside-specific IIBC component

Products: NA

Alternate protein names: Alpha-glucoside permease IIC component; PTS system alpha-glucoside-specific EIIC component; Alpha-glucoside-specific phosphotransferase enzyme IIB component; PTS system alpha-glucoside-specific EIIB component [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MRPQVLLNRLKKHLFFKDVFDAVYLCSQMSTFSRKRRSLPPNEKHESDHESHWVVMFCRSHLPILAPGLPHNQSGFLLTE
LRKEDVEMLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTDPNSLFAQIVHIIEEGGWTVFRNMPLIFAV
GLPIGLAKQAQGRACLAVMVSFLTWNYFINAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTAL
HNRLFDKKLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWVYTFLERILIPTGLHH
FIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGGFALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIP
ATLTAMLVGITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAIG
LCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRL
RIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV

Sequences:

>Translated_625_residues
MRPQVLLNRLKKHLFFKDVFDAVYLCSQMSTFSRKRRSLPPNEKHESDHESHWVVMFCRSHLPILAPGLPHNQSGFLLTE
LRKEDVEMLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTDPNSLFAQIVHIIEEGGWTVFRNMPLIFAV
GLPIGLAKQAQGRACLAVMVSFLTWNYFINAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTAL
HNRLFDKKLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWVYTFLERILIPTGLHH
FIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGGFALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIP
ATLTAMLVGITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAIG
LCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRL
RIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV
>Mature_625_residues
MRPQVLLNRLKKHLFFKDVFDAVYLCSQMSTFSRKRRSLPPNEKHESDHESHWVVMFCRSHLPILAPGLPHNQSGFLLTE
LRKEDVEMLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTDPNSLFAQIVHIIEEGGWTVFRNMPLIFAV
GLPIGLAKQAQGRACLAVMVSFLTWNYFINAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTAL
HNRLFDKKLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWVYTFLERILIPTGLHH
FIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGGFALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIP
ATLTAMLVGITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAIG
LCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRL
RIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV

Specific function: Involved in the transport and simultaneous phosphorylation at O-6 of the glucosyl moiety of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses. Can also transport maltose, isomaltose and maltitol, phosphorylating at O-6 of their non-reduci

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787908, Length=552, Percent_Identity=32.7898550724638, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787343, Length=530, Percent_Identity=30.7547169811321, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786894, Length=537, Percent_Identity=31.6573556797021, Blast_Score=169, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010975 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 68630; Mature: 68630

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPQVLLNRLKKHLFFKDVFDAVYLCSQMSTFSRKRRSLPPNEKHESDHESHWVVMFCRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
HLPILAPGLPHNQSGFLLTELRKEDVEMLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQ
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
NPMFVGESLTDPNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMV
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHCCCHHHHHHHH
SFLTWNYFINAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTAL
HHHHHHHHHHHHHCCHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRLFDKKLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGA
HHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
LGVWVYTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFP
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCC
EGGFALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
ISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHCCHHHHHHHHHHHH
LCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGI
HHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHH
LQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
QLREQLDSLINSHQSAENVAITEAV
HHHHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MRPQVLLNRLKKHLFFKDVFDAVYLCSQMSTFSRKRRSLPPNEKHESDHESHWVVMFCRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
HLPILAPGLPHNQSGFLLTELRKEDVEMLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQ
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
NPMFVGESLTDPNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMV
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHCCCHHHHHHHH
SFLTWNYFINAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTAL
HHHHHHHHHHHHHCCHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRLFDKKLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGA
HHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
LGVWVYTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFP
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCC
EGGFALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
ISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHCCHHHHHHHHHHHH
LCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGI
HHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHH
LQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
QLREQLDSLINSHQSAENVAITEAV
HHHHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11473129; 11322729 [H]