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The map label for this gene is betT [H]

Identifier: 157157763

GI number: 157157763

Start: 4840350

End: 4842353

Strand: Reverse

Name: betT [H]

Synonym: EcE24377A_4850

Alternate gene names: 157157763

Gene position: 4842353-4840350 (Counterclockwise)

Preceding gene: 157156882

Following gene: 157156131

Centisome position: 97.24

GC content: 50.25

Gene sequence:

>2004_bases
ATGATTATGAGTGAAAATGATACAATCCCAAAGAAGTCTACAAGTCAGATTAACAAAGCGGTATTCTTTACATCTGCTTT
GCTAATTTTCCTTCTTGTCGCCTTTGCCGCCGTATTCCCGGATGTCGCCGACAAAAATTTTAAACTACTTCAGCAACAAA
TCTTCACGAATGCCAGCTGGTTCTACATCCTTGCTGTGGCCCTGATTTTACTGAGTGTCACGTTCCTTGGACTCTCACGC
TACGGTGATATCAAGCTGGGCCCGGACCATGCGCAGCCTGATTTCAGCTACCACTCCTGGTTTGCGATGCTTTTTTCGGC
AGGGATGGGGATCGGCCTGATGTTCTTTGGCGTTGCCGAACCTGTAATGCATTATCTTTCGCCACCCGTTGGCACTCCAG
AAACCGTTGCGGCAGCTAAGGAAGCAATGCGTCTGACCTTTTTCCACTGGGGACTGCACGCATGGGCAATTTATGCCATT
GTGGCGCTGATTCTGGCGTTCTTCAGTTACCGTCACGGTCTGCCTTTAACTCTGCGCTCCGCACTCTATCCCATTATTGG
CGATCGCATATACGGACCTGTAGGACATGCGGTTGATATTTTCGCTGTTATAGGCACGGTCTTTGGCGTTTCGACATCAC
TGGGTTACGGTGTTTTGCAGGTGAATGCCGGTTTGAACCATCTTTTCGGGGTGCCCATCAATGAAACGGTGCAGGTAATT
CTGATCGTGGTCATCACGGGGTTAGCGACGATTTCAGTGGTGTCCGGTCTGGATAAGGGAATACGTATCCTGTCTGAACT
CAATCTGGGTCTGGCTTTGTTGCTCCTGGCGCTGGTCCTGTGTCTGGGACCAACCGTGCTTCTGCTGAAGTCATTTGTGG
AAAATACGGGCGGTTATCTTTCGGAACTGGTGAGTAAAACGTTCAACCTTTACGCGTATGAGCCCAAGTCGAGCAACTGG
CTGGGGGGCTGGACATTACTGTACTGGGGATGGTGGCTTTCATGGTCGCCGTTTGTGGGGATGTTCATCGCACGGGTCTC
CCGCGGGCGAACCATTCGCGAGTTTGTCACCGGCGTGCTGTTTGTTCCCGCGGGTTTTACGCTAATGTGGATGACGGTGT
TTGGTAACAGCGCGATCTATCTCATTATGAACCAGGGGGCCACAGACCTCGCCAATACTGTTCAGCAGGATGTGGCGCTG
GCCCTGTTTAATTTCCTGGAGCATTTCCCGTTCTCTTCTGTGCTGTCATTCATTGCAATGGCGATGGTCATCGTCTTCTT
TGTAACGTCTGCTGATTCGGGGGCAATGGTTGTGGATACTCTGGCATCAGGTGGAGTGGCAAACACACCCGTCTGGCAGC
GAATATTCTGGGCCTCGCTCATGGGCATTGTTGCAATTGCGCTTCTCCTTGCCGGAGGGCTAAGTGCGCTGCAAACGGTG
ACAATAGCGAGTGCATTGCCCTTCTCAGTGATCTTACTAATATCTATATACGGACTTTTAAAAGCTTTGCGCCGGGATTT
GACCAAGCGTGAAAGCCTGAGCATGGCGACAATTGCTCCTACGGCTGCACGTAACCCAATTCCTTGGCAGAGAAGGTTAC
GCAATATCGCGTATCTGCCGAAGCGATCTCTTGTGAAACGTTTTATGGACGACGTTATCCAGCCTGCCATGACGCTGGTT
CAGGAGGAACTGAACAAGCAGGGGACGATAAGCCACATTAGTGATGCAGTCGACGATCGTATTCGTCTTGAAGTCGATTT
GGGCAACGAGCTGAATTTTATATATGAAGTGAGGCTTCGCGGGTATATCTCACCGACCTTCGCGCTCGCCGCAATGGATA
ATGATGAGCAGCAGACTGAACAACATCGATATTATCGCGCTGAGGTTTATCTCAAAGAAGGCGGTCAAAATTATGATGTG
ATGGGCTGGAACCAGGAACAGCTGATTAATGACATACTGGACCAGTACGAAAAACACCTGCACTTCCTGCACCTGGTTCG
TTAA

Upstream 100 bases:

>100_bases
TCCACCATGCATTACGATTAACGCCGCATAGCCAGTTGAACTTTGCTACTTTGTGAGAGGTAGTACCTTCTATCCAGTGC
GAATTTAATTAATGGAATAA

Downstream 100 bases:

>100_bases
TAGCAACATGCCGTCCTGGGGGCGGCAATTATTATCCCGGCCGCAATATGAGGGAATGCAGAATGATTTCACGCTGGAAA
TGGATGCTGAAGCAGACAAT

Product: BCCT family transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MIMSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNFKLLQQQIFTNASWFYILAVALILLSVTFLGLSR
YGDIKLGPDHAQPDFSYHSWFAMLFSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAI
VALILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVSTSLGYGVLQVNAGLNHLFGVPINETVQVI
LIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNW
LGGWTLLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVAL
ALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTV
TIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLV
QEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDV
MGWNQEQLINDILDQYEKHLHFLHLVR

Sequences:

>Translated_667_residues
MIMSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNFKLLQQQIFTNASWFYILAVALILLSVTFLGLSR
YGDIKLGPDHAQPDFSYHSWFAMLFSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAI
VALILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVSTSLGYGVLQVNAGLNHLFGVPINETVQVI
LIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNW
LGGWTLLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVAL
ALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTV
TIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLV
QEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDV
MGWNQEQLINDILDQYEKHLHFLHLVR
>Mature_667_residues
MIMSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNFKLLQQQIFTNASWFYILAVALILLSVTFLGLSR
YGDIKLGPDHAQPDFSYHSWFAMLFSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAI
VALILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVSTSLGYGVLQVNAGLNHLFGVPINETVQVI
LIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNW
LGGWTLLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVAL
ALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTV
TIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLV
QEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDV
MGWNQEQLINDILDQYEKHLHFLHLVR

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=672, Percent_Identity=41.6666666666667, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788102, Length=447, Percent_Identity=30.4250559284116, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786224, Length=504, Percent_Identity=27.1825396825397, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 73989; Mature: 73989

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNFKLLQQQIFTNASW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
FYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAMLFSAGMGIGLMFFGVAE
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
PVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVALILAFFSYRHGLPLTLRS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALYPIIGDRIYGPVGHAVDIFAVIGTVFGVSTSLGYGVLQVNAGLNHLFGVPINETVQVI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCCHHHHHHH
LIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPTVLLLKSFVENTGGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
SELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVL
HHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
FVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVALALFNFLEHFPFSSVLSFIAM
HHCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTV
HHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHCCC
KRSLVKRFMDDVIQPAMTLVQEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCCCEEEEEEEC
GYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHL
CCCCHHHEEEECCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFLHLVR
HHHHHCC
>Mature Secondary Structure
MIMSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKNFKLLQQQIFTNASW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHH
FYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAMLFSAGMGIGLMFFGVAE
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
PVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVALILAFFSYRHGLPLTLRS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALYPIIGDRIYGPVGHAVDIFAVIGTVFGVSTSLGYGVLQVNAGLNHLFGVPINETVQVI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCCHHHHHHH
LIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPTVLLLKSFVENTGGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
SELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVL
HHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
FVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVALALFNFLEHFPFSSVLSFIAM
HHCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTV
HHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIASALPFSVILLISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHCCC
KRSLVKRFMDDVIQPAMTLVQEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCCCEEEEEEEC
GYISPTFALAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHL
CCCCHHHEEEECCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFLHLVR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]