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The map label for this gene is msbB2 [H]

Identifier: 157157717

GI number: 157157717

Start: 4843821

End: 4844765

Strand: Reverse

Name: msbB2 [H]

Synonym: EcE24377A_4852

Alternate gene names: 157157717

Gene position: 4844765-4843821 (Counterclockwise)

Preceding gene: 157155751

Following gene: 157156882

Centisome position: 97.29

GC content: 42.54

Gene sequence:

>945_bases
ATGAAAAAATATAAATCTGAGTTTATTCCTGAATTTAAGAAAAATTACCTTTCCCCTGTTTACTGGTCTACATGGTTCCT
TTTGGGAATGATTGCAGGTATTTCAATGTTTCCCCCTTCATTCAGAGATCCTGTCTTGGCAAAAATAGGGCGTTGGGCGG
GCAGATTGAGCAAAAAAGCTCGTCGCAGGGCGACGATTAATTTATCGCTTTGTTTCCCAGAAAAGAGTGATACAGAACGG
GAAATAATTGTCGACAAAATGTTTGTCACAGCATTGCAATCCATAGTGATGATGGCAGAACTGGCAATTCGTGGTCCGGA
AAAGTTCCAGAAACGAGTGTTCTGGAAAGGCCTCGAAATTCTTGAGGAAATTCAACACAATAATAGAAATGTGATTTTTC
TGGTTCCCCATAGCTGGAGCGTGGATATTCCTGCAATGTTGCTGGCAGCTCAGGGAAAAAAAATGGCCGCCATGTTTCAT
CAGCAACGAAATCCAGTGATTGATTATGTCTGGAATTCAGTACGGCGTAAATTCGGGGGGCGCTTACATGCCCGGGAGGA
TGGGATAAAACCATTTATTCAGTCAGTACGCCAGGGATACTGGGGATATTACCTCCCAGATCAGGATCATGGTCCTGAAT
ACAGTGAATTTGCTGATTTTTTTGCGACCTATAAAGCGACATTACCAATCATTGGACGTCTGATGAACATCAGTCAGGCT
ATGATTATACCACTTTTCCCGGTTTATGATGAAAAAAAACATCTACTGACTATTGAAATTCGGCCACCAATGGATGCTTG
CATTGCCAGCGCGGACAATAAAACGATTGCCCGACAAATGAACAAAACAGTGGAAATTTTGGTGGGGCCACATCCGGAAC
AGTATGTCTGGGTTTTAAAATTGTTAAAAACACGCAAATCAAACGAAGCGGACCCGTACCCTTGA

Upstream 100 bases:

>100_bases
TATCCTGTACACTCAGAACATCAGAATTTAAATTAAGGTTTGATGTATTCTCTGAATAAAACAGTAATGATAATTTTGGT
AGAGGCATTCGCACTAAATA

Downstream 100 bases:

>100_bases
AATTATAAAGTACAAGTATTTCCACTAGTTGTTTGTAAGCGTACAGCCTGAACCGTCTGGTCAGAATCTGACGAATTAGA
CAAAGTGGTGTCCACCAAAT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: KDO2-lipid A, cold adapted; [acyl carrier protein]; KDO2-lipid A [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTER
EIIVDKMFVTALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIQHNNRNVIFLVPHSWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP

Sequences:

>Translated_314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTER
EIIVDKMFVTALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIQHNNRNVIFLVPHSWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP
>Mature_314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTER
EIIVDKMFVTALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIQHNNRNVIFLVPHSWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=312, Percent_Identity=69.8717948717949, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787292, Length=318, Percent_Identity=29.874213836478, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87082094, Length=308, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36455; Mature: 36455

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKA
CCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELAIRGPEKFQKRVFWKGLEI
HHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LEEIQHNNRNVIFLVPHSWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNSVRRKFGG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
RLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLK
HHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHH
LLKTRKSNEADPYP
HHHHHCCCCCCCCC
>Mature Secondary Structure
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKA
CCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELAIRGPEKFQKRVFWKGLEI
HHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LEEIQHNNRNVIFLVPHSWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNSVRRKFGG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
RLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLK
HHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHH
LLKTRKSNEADPYP
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-(palmitoleoyl)-lipid IVA; myristoyl-ACP; KDO2-(lauroyl)-lipid IVA [C]

Specific reaction: KDO2-(palmitoleoyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A, cold adapted + [acyl carrier protein] KDO2-(lauroyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A + [acyl carrier protein] [C]

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11115111; 11292750; 12384590; 9171415 [H]