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The map label for this gene is eptA [H]

Identifier: 157157656

GI number: 157157656

Start: 4665347

End: 4667020

Strand: Reverse

Name: eptA [H]

Synonym: EcE24377A_4668

Alternate gene names: 157157656

Gene position: 4667020-4665347 (Counterclockwise)

Preceding gene: 157154776

Following gene: 157158920

Centisome position: 93.72

GC content: 49.64

Gene sequence:

>1674_bases
ATGCGCACTTTGTTCGATGGAAACACCGTGATGTTGAAGCGCCTACTAAAAAGACCCTCTTTGAATTTACTCGCCTGGCT
ATTGTTGGCCGCTTTTTATATCTCTATCTGCCTGAATATTGCCTTTTTTAAACAGGTGTTGCAGGCGCTGCCGCTGGATT
CGCTGCATAACGTACTGGTTTTCTTGTCGATGCCGGTCGTCGCTTTCAGCGTGATTAATATTGTCCTGACACTAAGCTCT
TTCTTATGGCTTAATCGACCACTGGCCTGCCTGTTTATTCTGGTTGGCGCGGCTGCACAATATTTCATAATGACTTACGG
CATCGTCATCGACCGCTCGATGATTGCCAATATTATTGATACCACTCCGGCAGAAAGTTATGCGCTGATGACACCGCAAA
TGTTATTAACGCTGGGATTCAGCGGCGTGCTTGCTGCGCTGATTGCCTGCTGGATAAAAATCAAACCTGCCACCTCGCGT
CTGCGCAGTGTTCTTTTCCGTGGAGCCAATATTCTGGTTTCTGTACTACTGATTTTGCTGGTCGCCGCACTGTTTTATAA
AGACTACGCCTCGTTGTTCCGCAATAACAAAGAGCTGGTGAAATCCTTAAGCCCCTCTAACAGCATTGTTGCCAGCTGGT
CATGGTACTCCCATCAGCGACTGGCAAATCTACCGCTGGTGCGAATTGGTGAAGACGCCCACCGCAACCCGTTAATGCAG
AACGAAAAACGTAAAAATTTGACCATCCTGATTGTCGGCGAAACCTCGCGGGCGGAGAACTTCTCCCTCAACGGCTACCC
GCGTGAAACTAACCCGCGGCTGGCGAAAGATAACGTGGTCTATTTCCCTAATACCGCATCTTGCGGCACGGCAACGGCAG
TTTCAGTACCGTGCATGTTCTCGGATATGCCGCGTGAGCATTACAAAGAAGAGCTGGCACAGCACCAGGAAGGCGTGCTG
GATATCATTCAGCGAGCGGGCATCAACGTGCTGTGGAATGACAACGATGGCGGCTGTAAAGGTGCCTGCGACCGCGTGCC
TCACCAGAACGTCACCGCGCTGAATCTGCCTGGTCAGTGCATCAACGGCGAATGCTATGACGAAGTGCTGTTCCACGGGC
TGGAAGAGTACATCAATAACCTGCAGGGTGATGGCGTGATTGTCTTACACACCATCGGCAGCCACGGTCCGACCTATTAC
AACCGCTATCCGCCGCAGTTCAGGAAATTTACCCCAACCTGCGACACCAATGAGATCCAGACCTGTAGCAAAGAGCAACT
GGTGAACACTTACGACAACACGCTGGTTTACGTCGACTATATTGTTGATAAAGCGATTAATCTGCTGAAAGAACATCAGG
ATAAATTTACCACCAGCCTGGTTTATCTTTCTGACCACGGTGAATCGTTAGGTGAAAATGGCATCTATCTGCACGGTCTG
CCTTATGCCATCGCCCCGGATAGCCAAAAACAGGTGCCGATGCTGCTGTGGCTGTCGGAGGATTATCAAAAACGGTATCA
GGTTGACCAGAACTGCCTGCAAAAACAGGCGCAAACGCAACACTATTCACAAGACAATTTATTCTCCACGCTATTGGGAT
TAACTGGCGTTGAGACGAAGTATTACCAGGCTGCGGATGATATTCTGCAAACTTGCAGGAGAGTGAGTGAATGA

Upstream 100 bases:

>100_bases
TTAGATGCACCAATAAGCAAAGATTAATTCCCCTTAATCCAGCAAACATAAAAGCCAACCTTAAGAACTTAAGGTTGGCT
TAATTTTGCTTTGCGAGCAT

Downstream 100 bases:

>100_bases
AAATTCTGATTGTTGAAGACGATACGCTGTTATTGCAGGGATTGATTCTGGCGGCGCAAACTGAAGGCTACGCGTGCGAT
GGCGTGACAACCGCGCGGAT

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MRTLFDGNTVMLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSS
FLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSR
LRSVLFRGANILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQ
NEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVL
DIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYY
NRYPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGL
PYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

Sequences:

>Translated_557_residues
MRTLFDGNTVMLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSS
FLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSR
LRSVLFRGANILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQ
NEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVL
DIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYY
NRYPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGL
PYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE
>Mature_557_residues
MRTLFDGNTVMLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSS
FLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSR
LRSVLFRGANILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQ
NEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVL
DIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYY
NRYPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGL
PYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=547, Percent_Identity=99.6343692870201, Blast_Score=1131, Evalue=0.0,
Organism=Escherichia coli, GI87082286, Length=546, Percent_Identity=24.3589743589744, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082223, Length=462, Percent_Identity=24.8917748917749, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62731; Mature: 62731

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLFDGNTVMLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLV
CCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIID
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILL
CCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
VAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQ
HHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCEEEECCCHHCCCCCC
NEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHEECCHHH
SDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPGQC
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCEEECCCCCC
INGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQ
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHH
TCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGL
HCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECC
PYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETK
CEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
YYQAADDILQTCRRVSE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTLFDGNTVMLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLV
CCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIID
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILL
CCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
VAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQ
HHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCEEEECCCHHCCCCCC
NEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHEECCHHH
SDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPGQC
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCEEECCCCCC
INGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQ
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHH
TCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGL
HCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECC
PYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETK
CEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
YYQAADDILQTCRRVSE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]