Click here to switch to the map view.

The map label for this gene is plsB

Identifier: 157157568

GI number: 157157568

Start: 4593069

End: 4595492

Strand: Reverse

Name: plsB

Synonym: EcE24377A_4594

Alternate gene names: 157157568

Gene position: 4595492-4593069 (Counterclockwise)

Preceding gene: 157158569

Following gene: 157154721

Centisome position: 92.29

GC content: 55.16

Gene sequence:

>2424_bases
ATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGC
AGATCCTGCCCCGGAACTGGGGCTGGATACCTCTCGTCCAATTATGTACGTTTTACCGTACAACTCGAAAGCAGATTTGC
TGACGTTGCGCGCCCAGTGTCTGGCTCATGACTTGCCTGACCCGTTAGAGCCGCTGGAAATCGACGGCACGCTACTGCCG
CGCTATGTGTTCATTCACGGCGGGCCGCGTGTGTTCACCTATTACACGCCGAAAGAAGAGTCTATTAAGCTGTTCCACGA
CTATCTTGATTTGCACCGCAGCAACCCAAATCTGGATGTGCAGATGGTGCCAGTATCGGTGATGTTTGGTCGCGCGCCGG
GGCGTGAAAAAGGCGAAGTAAACCCGCCGCTGCGTATGCTTAACGGCGTACAAAAATTTTTCGCCGTACTGTGGCTCGGT
CGCGACAGTTTTGTGCGTTTCTCGCCGTCAGTTTCTCTGCGCCGCATGGCCGATGAACACGGCACAGATAAAACTATCGC
TCAGAAACTGGCGCGCGTGGCGCGTATGCACTTTGCCCGTCAACGTCTGGCTGCCGTAGGCCCACGTCTGCCTGCTCGTC
AGGATCTGTTTAATAAGCTGCTCGCCTCCCGCGCCATTGCCAAAGCGGTAGAAGATGAAGCGCGCAGCAAAAAAATCTCC
CATGAAAAAGCGCAGCAGAACGCGATTGCGCTGATGGAAGAGATTGCGGCAAATTTCTCTTACGAGATGATCCGCCTGAC
TGACCGTATTCTGGGCTTCACCTGGAACCGACTTTACCAGGGCATCAACGTCCATAACGCCGAGCGCGTTCGCCAGCTGG
CCCACGACGGTCATGAGCTAGTGTATGTGCCTTGCCACCGCAGTCACATGGACTACCTGCTACTTTCTTACGTGCTGTAC
CACCAGGGGCTGGTGCCGCCGCATATCGCCGCCGGGATCAACCTGAACTTCTGGCCGGCCGGGCCGATTTTCCGCCGTCT
GGGGGCGTTCTTTATTCGCCGTACTTTTAAAGGCAATAAACTTTATTCCACCGTTTTCCGTGAGTATCTCGGCGAACTGT
TCAGCCGTGGTTATTCCGTCGAGTACTTCGTGGAAGGCGGTCGTTCCCGCACGGGGCGTTTGCTGGATCCGAAAACCGGC
ACGCTGTCGATGACCATTCAGGCGATGCTGCGTGGCGGCACGCGTCCGATTACGCTGATTCCGATCTATATCGGTTATGA
GCACGTCATGGAAGTGGGTACTTACGCCAAAGAACTGCGCGGCGCGACGAAAGAGAAAGAGAGCCTGCCGCAGATGCTGC
GCGGTTTAAGCAAGCTGCGTAATCTCGGTCAGGGTTACGTCAACTTCGGTGAACCAATGCCGTTGATGACCTACCTTAAC
CAGCACGTACCAGACTGGCGTGAATCTATCGATCCCATCGAAGCGGTGCGTCCGGCCTGGTTAACGCCGACGGTCAATAA
TATTGCTGCCGATCTGATGGTACGCATTAACAACGCAGGCGCGGCAAACGCCATGAACCTGTGTTGTACTGCGCTTCTGG
CATCACGTCAGCGCTCACTCACCCGCGAGCAGTTAACCGAGCAACTCAACTGCTACCTGGATCTGATGCGCAACGTACCT
TACTCCACGGACTCTACCGTTCCTTCAGCCAGCGCCAGCGAGCTTATCGATCACGCGCTGCAAATGAACAAGTTTGAAGT
CGAGAAAGACACTATCGGCGACATCATCATTCTGCCACGCGAGCAAGCGGTGCTGATGACCTACTATCGCAACAACATTG
CGCATATGTTGGTGCTGCCTTCGCTGATGGCGGCAATTGTCACCCAGCATCGCCACATCTCCCGCGACGTATTGATGGAG
CACGTCAATGTGCTTTACCCAATGCTGAAAGCGGAGCTGTTCCTGCGCTGGGATCGCGACGAGTTGCCGGACGTTATTGA
TGCGCTGGCAAATGAGATGCAACGTCAGGGGCTGATTACCCTGCAAGATGATGAGTTGCATATCAACCCGGCGCATTCTC
GCACGCTACAGCTGCTGGCCGCAGGCGCGCGCGAAACGCTGCAACGTTATGCCATCACCTTCTGGTTGTTGAGTGCCAAC
CCGTCGATCAACCGCGGTACGCTGGAGAAAGAGAGCCGCACCGTCGCGCAACGTCTCTCCGTGCTGCACGGCATCAACGC
GCCGGAGTTCTTCGACAAGGCGGTGTTCAGTTCTCTGGTGCTGACACTGCGTGATGAAGGGTATATCAGCGATAGCGGCG
ATGCCGAACCGGCAGAAACGATGAAGGTTTATCAGTTGCTGGCGGAGTTGATTACATCAGACGTGCGTTTGACGATTGAG
AGTGCGACGCAGGGCGAAGGGTAA

Upstream 100 bases:

>100_bases
AACGCCGCGCGAAACATGAGCGGATACCACAGAATTTCCCATGACTTTCTGCTATCCTTGCCGCGCATTTGCATTATTAA
CCAGAGGCTTTACATCGTTT

Downstream 100 bases:

>100_bases
TCAGAGAGAATTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTTCCATAATCTGCAAATTCAATAAATTGCGATGTGTT
GTGTAGGCCTGAGAAGCGCC

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLP
RYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKIS
HEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLY
HQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTG
TLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLN
QHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVP
YSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLME
HVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSAN
PSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIE
SATQGEG

Sequences:

>Translated_807_residues
MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLP
RYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKIS
HEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLY
HQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTG
TLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLN
QHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVP
YSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLME
HVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSAN
PSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIE
SATQGEG
>Mature_806_residues
SGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPR
YVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGR
DSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISH
EKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYH
QGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGT
LSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQ
HVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPY
STDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEH
VNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANP
SINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIES
ATQGEG

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=435, Percent_Identity=27.816091954023, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI190358539, Length=272, Percent_Identity=33.4558823529412, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI87082362, Length=807, Percent_Identity=100, Blast_Score=1665, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71988723, Length=278, Percent_Identity=31.6546762589928, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147672, Length=279, Percent_Identity=30.1075268817204, Blast_Score=140, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71988728, Length=190, Percent_Identity=37.8947368421053, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17864692, Length=270, Percent_Identity=30.7407407407407, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24650752, Length=499, Percent_Identity=25.4509018036072, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21357731, Length=499, Percent_Identity=25.4509018036072, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24650754, Length=499, Percent_Identity=25.4509018036072, Blast_Score=144, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_ECOK1 (A1AIL8)

Other databases:

- EMBL:   CP000468
- RefSeq:   YP_859632.1
- ProteinModelPortal:   A1AIL8
- STRING:   A1AIL8
- EnsemblBacteria:   EBESCT00000035964
- GeneID:   4493032
- GenomeReviews:   CP000468_GR
- KEGG:   ecv:APECO1_2428
- eggNOG:   COG2937
- GeneTree:   EBGT00050000010697
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   ECOL405955:APECO1_2428-MONOMER
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 91383; Mature: 91252

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQC
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDV
HHCCCCCCCCCCCCCCEEECEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEE
QMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEH
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHC
GTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKIS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHEL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNK
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
LYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLI
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLN
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSL
HCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
TREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECC
EQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRD
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
ELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSAN
HHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAET
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
MKVYQLLAELITSDVRLTIESATQGEG
HHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQC
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDV
HHCCCCCCCCCCCCCCEEECEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEE
QMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEH
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHC
GTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKIS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHEL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNK
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
LYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLI
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLN
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSL
HCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
TREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECC
EQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRD
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
ELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSAN
HHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAET
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
MKVYQLLAELITSDVRLTIESATQGEG
HHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA