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The map label for this gene is cptA [H]

Identifier: 157157305

GI number: 157157305

Start: 4482843

End: 4484576

Strand: Reverse

Name: cptA [H]

Synonym: EcE24377A_4494

Alternate gene names: 157157305

Gene position: 4484576-4482843 (Counterclockwise)

Preceding gene: 157159345

Following gene: 157157214

Centisome position: 90.06

GC content: 50.46

Gene sequence:

>1734_bases
ATGCATTCCACAGAAGTCCAGGCTAAACCCCTTTTTAGCTGGAAAGCCCTGGGTTGGGCACTGCTCTACTTTTGGTTTTT
CTCTACTCTGCTACAGGCCATTATTTACATCAGTGGTTATAGTGGCACCAACGGCATTCGCGACTCGCTGTTATTCAGCT
CGCTGTGGTTGATCCCGGTATTCCTCTTTCCGAAGCGGATTAAAATTATTGCCGCAGTAATCGGCGTGGTGCTATGGGCG
GCCTCTCTGGCGGCGCTATGCTACTACGTCATCTACGGGCAGGAGTTCTCGCAGAGCGTTCTGTTTGTGATGTTCGAAAC
CAACACCAACGAAGCCAGCGAGTATTTAAGCCAGTATTTCAGCCTGAAAATTGTGCTTATCGCGCTGGCCTATACGGCAA
TGGCAGTTCTGCTGTGGACACGTCTGCGCCCGGTCTATATTCCAAAGCCGTGGCGTTATATTGTCTCTTTTGCCCTGCTT
TATGGCTTGATTCTGCATCCGATCGCCATGAATACGTTTATCAAAAACAAGCCGTTTGAGAAAACGTTGGATAACCTGGC
CTCGCGTATGGAGCCTGCCGCACCGTGGCAATTCCTGACCGGCTATTATCAGTATCGTCAGCAACTAAACTCGCTAACAA
AGCTACTGAATGAAAATAATGCCTTGCCGCCGCTGGCTAATTTCAAAGATGAATCGGGTAACGAACCGCGCACCTTAGTG
CTGGTGATTGGCGAGTCGACCCAGCGTGGACGCATGAGTCTGTACGGTTATCCGCGTGAAACCACGCCGGAGCTGGATGC
GCTGCATAAAACCGATCCGAATCTGACCGTGTTTAATAACGTAGTTACGTCTCGTCCGTACACCATTGAAATCCTGCAAC
AGGCGCTGACCTTTGCCAATGAAAAGAACCCGGATCTGTATCTGACGCAGCCGTCGCTGATGAACATGATGAAACAGGCG
GGTTATAAAACCTTCTGGATCACCAACCAGCAGACGATGACCGCCCGCAATACCATGCTGACGGTATTTTCGCGCCAGAC
CGACAAGCAGTACTACATGAACCAGCAACGTACGCAGAGTGCGCGTGAATACGACACCAACGTGCTGAAGCCGTTCCAGG
ATGTGCTGAATGACCCTGCGCCGAAGAAACTGATCATCGTTCATCTGCTGGGTACGCATATCAAATACAAATACCGCTAC
CCGGAAAATCAGGGCAAGTTTGATGGCAATACCGATCATGTTCCGCCAGGATTAAGCGCAGAAGAGCTGGAATCATATAA
CGATTATGACAACGCTAACCTGTATAACGATCATGTGGTTGCCAGCCTGATTAAAGACTTTAAAGCGGCAGACCCGAACG
GATTCCTGGTTTACTTCTCTGACCACGGTGAAGAGGTTTACGACACGCCGCCGCACAAAACTCAGGGGCGTAACGAAGAC
AACCCGACGCGCCACATGTACACCATTCCGTTCCTGCTGTGGACGTCGGAAAAATGGCAAGCGACTCATCCCCGTGATTT
CTCACAGGATGTCGATCGTAAATACAGCCTGGCGGAACTGATCCACACCTGGTCAGATTTGGCGGGCTTATCTTACGACG
GTTACGATCCAACCCGTTCAGTGGTGAATCCGCAGTTCAAAGAAACTACCCGCTGGATTGGTAACCCGTACAAGAAAAAC
GCGCTGATCGATTACGACACTCTGCCGTATGGCGATCAGGTGGGTAATCAGTAA

Upstream 100 bases:

>100_bases
TTTCTGTCTGCATTTTATTCAAATTCTGAATATACCTTCAGATATCCTTAAGGAATTGTCGTTACATTCGGCGATATTTT
TTTCAAGACAGGTTCTTACT

Downstream 100 bases:

>100_bases
ATGACCTTTGTATCGCGTCTGGCGCTATTTGCCGGATGCGATACTGCCGCAATGTTTACATCGAACTCCCCTCAGGCCAC
ACTTTTCAGGTGGCCTTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTNGIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWA
ASLAALCYYVIYGQEFSQSVLFVMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNENNALPPLANFKDESGNEPRTLV
LVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLTVFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQA
GYKTFWITNQQTMTARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGTHIKYKYRY
PENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAADPNGFLVYFSDHGEEVYDTPPHKTQGRNED
NPTRHMYTIPFLLWTSEKWQATHPRDFSQDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKN
ALIDYDTLPYGDQVGNQ

Sequences:

>Translated_577_residues
MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTNGIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWA
ASLAALCYYVIYGQEFSQSVLFVMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNENNALPPLANFKDESGNEPRTLV
LVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLTVFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQA
GYKTFWITNQQTMTARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGTHIKYKYRY
PENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAADPNGFLVYFSDHGEEVYDTPPHKTQGRNED
NPTRHMYTIPFLLWTSEKWQATHPRDFSQDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKN
ALIDYDTLPYGDQVGNQ
>Mature_577_residues
MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTNGIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWA
ASLAALCYYVIYGQEFSQSVLFVMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNENNALPPLANFKDESGNEPRTLV
LVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLTVFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQA
GYKTFWITNQQTMTARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGTHIKYKYRY
PENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAADPNGFLVYFSDHGEEVYDTPPHKTQGRNED
NPTRHMYTIPFLLWTSEKWQATHPRDFSQDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKN
ALIDYDTLPYGDQVGNQ

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the outer membrane lipopolysaccharide core [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. CptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790392, Length=577, Percent_Identity=99.1334488734835, Blast_Score=1188, Evalue=0.0,
Organism=Escherichia coli, GI87082223, Length=476, Percent_Identity=23.9495798319328, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI87082286, Length=281, Percent_Identity=25.2669039145907, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787035, Length=503, Percent_Identity=20.8747514910537, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 66617; Mature: 66617

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTNGIRDSLLFSSLWLIPV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLFVMFETNTNEASEYLSQYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHH
SLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALLYGLILHPIAMNTFIKNKPFE
HHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNENNALPPLANFKDESGNEPRTLV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
LVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLTVFNNVVTSRPYTIEILQQALTFAN
EEECCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCEEEHHHHCCCCHHHHHHHHHHHHCC
EKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMTARNTMLTVFSRQTDKQYYMNQQRTQS
CCCCCEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHEEEHHCCCCHHHHHHHHHHHH
AREYDTNVLKPFQDVLNDPAPKKLIIVHLLGTHIKYKYRYPENQGKFDGNTDHVPPGLSA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCH
EELESYNDYDNANLYNDHVVASLIKDFKAADPNGFLVYFSDHGEEVYDTPPHKTQGRNED
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NPTRHMYTIPFLLWTSEKWQATHPRDFSQDVDRKYSLAELIHTWSDLAGLSYDGYDPTRS
CCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VVNPQFKETTRWIGNPYKKNALIDYDTLPYGDQVGNQ
HCCCCHHHHHHHHCCCHHCCCEEECCCCCCCHHCCCC
>Mature Secondary Structure
MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTNGIRDSLLFSSLWLIPV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLFVMFETNTNEASEYLSQYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHH
SLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALLYGLILHPIAMNTFIKNKPFE
HHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNENNALPPLANFKDESGNEPRTLV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
LVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLTVFNNVVTSRPYTIEILQQALTFAN
EEECCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCEEEHHHHCCCCHHHHHHHHHHHHCC
EKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMTARNTMLTVFSRQTDKQYYMNQQRTQS
CCCCCEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHEEEHHCCCCHHHHHHHHHHHH
AREYDTNVLKPFQDVLNDPAPKKLIIVHLLGTHIKYKYRYPENQGKFDGNTDHVPPGLSA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCH
EELESYNDYDNANLYNDHVVASLIKDFKAADPNGFLVYFSDHGEEVYDTPPHKTQGRNED
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NPTRHMYTIPFLLWTSEKWQATHPRDFSQDVDRKYSLAELIHTWSDLAGLSYDGYDPTRS
CCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VVNPQFKETTRWIGNPYKKNALIDYDTLPYGDQVGNQ
HCCCCHHHHHHHHCCCHHCCCEEECCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]