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The map label for this gene is cysP

Identifier: 157157262

GI number: 157157262

Start: 2699559

End: 2700575

Strand: Reverse

Name: cysP

Synonym: EcE24377A_2711

Alternate gene names: 157157262

Gene position: 2700575-2699559 (Counterclockwise)

Preceding gene: 157158523

Following gene: 157157732

Centisome position: 54.23

GC content: 53.79

Gene sequence:

>1017_bases
ATGGCCGTTAACTTACTGAAAAAGAACTCACTCGCGCTGGTCGCTTCTCTGCTGCTGGCGGGCCATGTACAGGCAACGGA
ACTGCTGAACAGTTCTTATGACGTCTCCCGCGAGCTGTTTGCCGCCCTGAATCCGCCGTTTGAGCAACAATGGGCAAAAG
ATAACGGCGGCGACAAACTGACGATAAAACAATCTCATGCCGGGTCATCAAAACAGGCGCTGGCGATTTTACAGGGCTTA
AAAGCCGACGTTGTCACTTATAACCAGGTGACCGACGTACAAATCCTGCACGATAAAGGCAAGCTGATCCCGGCCGACTG
GCAGTCGCGCCTGCCGAATAATAGCTCGCCGTTCTACTCCACCATGGGCTTCCTGGTGCGTAAGGGTAACCCGAAGAATA
TCCACGATTGGAACGACCTGGTGCGCTCCGACGTGAAGCTGATTTTCCCGAACCCGAAAACGTCGGGTAACGCGCGTTAT
ACCTATCTGGCGGCATGGGGCGCAGCGGATAAAGCTGACGGTGGTGACAAAGGCAAAACCGAACAGTTTATGACCCAGTT
CCTGAAAAACGTTGAAGTGTTCGATACTGGCGGTCGTGGCGCGACCACCACTTTTGCCGAGCGCGGCCTGGGCGATGTGC
TGATTAGCTTCGAATCGGAAGTGAACAACATCCGTAAACAGTATGAAGCGCAGGGCTTTGAAGTGGTGATTCCGAAAACC
AACATTCTGGCGGAATTCCCGGTGGCGTGGGTTGATAAAAACGTGCAGGCCAACGGTACGGAAAAAGCCGCCAAAGCCTA
TCTGAACTGGCTCTATAGCCCGCAGGCGCAAACCATCATCACCGACTATTACTACCGCGTGAATAACCCGGAGGTGATGG
ACAAACTGAAAGACAAATTCCCGCAGACCGAGCTGTTCCGCGTGGAAGACAAATTTGGCTCCTGGCCGGAAGTGATGAAA
ACCCACTTCACCAGCGGCGGCGAGTTAGACAAGCTGTTAGCGGCGGGGCGTAACTGA

Upstream 100 bases:

>100_bases
ACTTCCAAATCACCAAACGGTATATAAAACCGTTACTCCTTTCACGTCCGTTATAAATATGATGGCTATTAGAAAGTCAT
TAAATTTATAAGGGTGCGCA

Downstream 100 bases:

>100_bases
TGTTTGCTGTCTCCTCCAGACGCGTGCTGCCGGGCTTTACCTTAAGCCTCGGCACCAGTCTGCTGTTTGTGTGCCTGATT
TTGCTGCTGCCGCTCTCCGC

Product: thiosulfate transporter subunit

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRN

Sequences:

>Translated_338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRN
>Mature_337_residues
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLK
ADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYT
YLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKTN
ILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKT
HFTSGGELDKLLAAGRN

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family

Homologues:

Organism=Escherichia coli, GI1788765, Length=338, Percent_Identity=100, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1790351, Length=323, Percent_Identity=46.1300309597523, Blast_Score=300, Evalue=1e-82,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSP_ECOLI (P16700)

Other databases:

- EMBL:   M32101
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A35403
- RefSeq:   AP_003019.1
- RefSeq:   NP_416920.1
- ProteinModelPortal:   P16700
- SMR:   P16700
- DIP:   DIP-9384N
- IntAct:   P16700
- STRING:   P16700
- SWISS-2DPAGE:   P16700
- PRIDE:   P16700
- EnsemblBacteria:   EBESCT00000000952
- EnsemblBacteria:   EBESCT00000015683
- GeneID:   946883
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2418
- KEGG:   eco:b2425
- EchoBASE:   EB0192
- EcoGene:   EG10195
- eggNOG:   COG4150
- GeneTree:   EBGT00050000009849
- HOGENOM:   HBG291868
- OMA:   FNAYNPV
- ProtClustDB:   PRK10852
- BioCyc:   EcoCyc:CYSP-MONOMER
- Genevestigator:   P16700
- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669
- TIGRFAMs:   TIGR00971

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 37615; Mature: 37484

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00401 PROK_SULFATE_BIND_1; PS00757 PROK_SULFATE_BIND_2; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKGKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRN
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKGKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRN
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646