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The map label for this gene is tdh [H]

Identifier: 157157199

GI number: 157157199

Start: 4109452

End: 4110477

Strand: Reverse

Name: tdh [H]

Synonym: EcE24377A_4120

Alternate gene names: 157157199

Gene position: 4110477-4109452 (Counterclockwise)

Preceding gene: 157157713

Following gene: 157158485

Centisome position: 82.55

GC content: 52.63

Gene sequence:

>1026_bases
ATGAAAGCGTTATCCAAACTGAAAGCGGAAGAGGGCATCTGGATGACCGACGTTCCTGTACCGGAACTCGGGCATAACGA
TCTGCTGATTAAAATCCGTAAAACAGCCATCTGCGGGACTGACGTTCACATCTATAACTGGGATGAGTGGTCGCAAAAAA
CCATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCATG
ATTGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGCGGTCACTGTCGCAACTGTCGTGGCGGTCGTACCCATCTGTG
CCGCAACACGATCGGCGTCGGCGTTAACCGCCCGGGCTGCTTCGCCGAATATCTGGTGATTCCGGCGTTCAACGCCTTCA
AAATTCCCGACAATATCTCCGACGACCTGGCTTCCATTTTTGATCCCTTCGGTAACGCCGTGCATACCGCGCTGTCGTTC
GATCTGGTTGGCGAGGATGTGCTGGTCTCTGGTGCAGGCCCGATTGGTATTATGGCAGCGGCGGTGGCGAAACACGTTGG
TGCACGCAATGTGGTGATCACTGATGTTAACGAATATCGTCTCGAACTGGCGCGCAAAATGGGTATCACCCGTGCGGTCA
ACGTCGCTAAAGAAAACCTTAATGATGTGATGGCAGAACTGGGCATGACCGAAGGCTTTGATGTCGGTCTGGAAATGCCC
GGTGCGCCGCCAGCGTTTCGTACCATGCTCGACACCATGAACCACGGCGGTCGCATTGCGATGCTGGGTATTCCACCGTC
CGATATGTCTATCGACTGGACCAAAGTAATCTTTAAAGGCTTGTTCATTAAAGGTATTTACGGTCGTGAGATGTTTGAAA
CCTGGTACAAGATGGCGGCGCTGATTCAGTCAGGTCTGGATCTCTCGCCGATCATTACCCATCGTTTCTCTATCGATGAT
TTCCAGAAGGGCTTTGACGCTATGCGTTCGGGCCAGTCCGGGAAAGTAATTCTGAGTTGGGATTAA

Upstream 100 bases:

>100_bases
CCAGATGTCTGCGGCGCATACCCCTGAGCAAATTACGCGTGCAGTAGAAGCATTCACGCGTATTGGTAAACAACTGGGCG
TTATCGCCTGAGGATGTGAG

Downstream 100 bases:

>100_bases
CACGAACAAGGGCTGGTATTCCAGCCCTTTTGCCTGAGGATAATCCGTTAAATATGTAAAATCCTGTCAGTGTAATAAAG
ATATCGTAATTGCGCTGATC

Product: L-threonine 3-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHIYNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFM
IGDRVSGEGHITCGHCRNCRGGRTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAELGMTEGFDVGLEMP
GAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKVIFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDD
FQKGFDAMRSGQSGKVILSWD

Sequences:

>Translated_341_residues
MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHIYNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFM
IGDRVSGEGHITCGHCRNCRGGRTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAELGMTEGFDVGLEMP
GAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKVIFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDD
FQKGFDAMRSGQSGKVILSWD
>Mature_341_residues
MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHIYNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFM
IGDRVSGEGHITCGHCRNCRGGRTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAELGMTEGFDVGLEMP
GAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKVIFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDD
FQKGFDAMRSGQSGKVILSWD

Specific function: Threonine catabolism; first step. [C]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=335, Percent_Identity=27.1641791044776, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4501929, Length=373, Percent_Identity=24.9329758713137, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI34577061, Length=362, Percent_Identity=24.3093922651934, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI262073058, Length=353, Percent_Identity=26.3456090651558, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI71743840, Length=353, Percent_Identity=26.3456090651558, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4501933, Length=373, Percent_Identity=23.5924932975871, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI156523966, Length=360, Percent_Identity=23.3333333333333, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI4501939, Length=354, Percent_Identity=23.1638418079096, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI71565154, Length=371, Percent_Identity=24.5283018867925, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790045, Length=341, Percent_Identity=99.1202346041056, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1788075, Length=366, Percent_Identity=30.0546448087432, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788073, Length=329, Percent_Identity=28.8753799392097, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1787863, Length=318, Percent_Identity=29.874213836478, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082125, Length=344, Percent_Identity=31.1046511627907, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI226510992, Length=330, Percent_Identity=27.5757575757576, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788407, Length=344, Percent_Identity=26.453488372093, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081918, Length=286, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790718, Length=321, Percent_Identity=26.1682242990654, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786825, Length=400, Percent_Identity=28, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786552, Length=358, Percent_Identity=27.3743016759777, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786518, Length=282, Percent_Identity=26.5957446808511, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=336, Percent_Identity=32.4404761904762, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17562878, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71988145, Length=233, Percent_Identity=31.7596566523605, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25146526, Length=380, Percent_Identity=24.7368421052632, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=273, Percent_Identity=26.7399267399267, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17562584, Length=122, Percent_Identity=36.8852459016393, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323099, Length=323, Percent_Identity=31.2693498452012, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322619, Length=325, Percent_Identity=27.6923076923077, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6319955, Length=325, Percent_Identity=27.3846153846154, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319258, Length=372, Percent_Identity=25.8064516129032, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319257, Length=335, Percent_Identity=27.1641791044776, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=26.1333333333333, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=324, Percent_Identity=29.320987654321, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=324, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=326, Percent_Identity=28.2208588957055, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=338, Percent_Identity=26.6272189349112, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323980, Length=258, Percent_Identity=29.4573643410853, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319949, Length=139, Percent_Identity=31.6546762589928, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17737897, Length=332, Percent_Identity=25.6024096385542, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137530, Length=332, Percent_Identity=25.6024096385542, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17737895, Length=377, Percent_Identity=24.9336870026525, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221457811, Length=168, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550770, Length=168, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551930, Length=168, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR004627
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.103 [H]

Molecular weight: Translated: 37269; Mature: 37269

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHIYNWDEWSQKTIPVPMV
CCHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCEEE
VGHEYVGEVVGIGQEVKGFMIGDRVSGEGHITCGHCRNCRGGRTHLCRNTIGVGVNRPGC
ECHHHHHHHHCCCHHHCCEEEECEECCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCH
FAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGEDVLVSGAGPIGIMAA
HHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHHHHH
AVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAELGMTEGFDVGLEMP
HHHHHCCCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
GAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKVIFKGLFIKGIYGREMFETWYKMAA
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSGKVILSWD
HHHCCCCCCHHHHEECCHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHIYNWDEWSQKTIPVPMV
CCHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCEEE
VGHEYVGEVVGIGQEVKGFMIGDRVSGEGHITCGHCRNCRGGRTHLCRNTIGVGVNRPGC
ECHHHHHHHHCCCHHHCCEEEECEECCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCH
FAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGEDVLVSGAGPIGIMAA
HHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHHHHH
AVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAELGMTEGFDVGLEMP
HHHHHCCCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
GAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKVIFKGLFIKGIYGREMFETWYKMAA
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSGKVILSWD
HHHCCCCCCHHHHEECCHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA