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The map label for this gene is cusR [H]

Identifier: 157156750

GI number: 157156750

Start: 618418

End: 619101

Strand: Reverse

Name: cusR [H]

Synonym: EcE24377A_0589

Alternate gene names: 157156750

Gene position: 619101-618418 (Counterclockwise)

Preceding gene: 157158754

Following gene: 157154804

Centisome position: 12.43

GC content: 54.24

Gene sequence:

>684_bases
ATGAAACTGTTGATTGTCGAAGATGAAAAGAAAACCGGAGAATACTTGACCAAAGGGTTAACCGAAGCCGGTTTTGTGGT
CGATTTGGCCGACAACGGGCTGAATGGCTACCATCTGGCGATGACCGGTGATTATGATCTGATAATCCTCGATATTATGC
TGCCGGACGTGAACGGCTGGGATATCGTACGCATGTTACGCTCCGCCAATAAAGGGATGCCGATTCTGTTGCTTACCGCG
CTTGGCACCATTGAACATCGCGTCAAGGGGCTGGAGTTGGGGGCAGATGACTACCTGATGAAACCATTCGCTTTTGCTGA
ACTGCTGGCGCGGGTGCGCACTCTCCTGCGGCGCGGGGCGGCGGTAATTATCGAAAGTCAGTTTCAGGTTGCCGACCTGA
TGGTCGATCTCGTCAGCCGCAAAGTCACCCGCAGCGGCACGCGCATCACTTTGACCAGTAAAGAGTTTACTCTGCTGGAG
TTTTTTCTCCGCCATCAGGGCGAAGTGCTGCCCCGCTCGCTTATCGCCTCGCAGGTGTGGGACATGAATTTCGACAGCGA
CACTAACGCCATTGATGTGGCGGTGAAGCGGCTACGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAGACCG
TGCGCGGCGTGGGCTATATGCTTGAGGTGCCGGATGGTCAGTAA

Upstream 100 bases:

>100_bases
TTCCGGTGACAGGAAAATGACAAAATTGTCATTTTGCCAATAAGCGATTGCCATCTGATCCCGCTACTCTAGAATTGCCC
GGGCAACATGCGGAGGAAAT

Downstream 100 bases:

>100_bases
GCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGTCTGACCTTTTTTATCAGCCTGGCCACCATCGCGGCGTTTTTCGCCT
TTGCATGGATCATGATCCAC

Product: DNA-binding transcriptional activator CusR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLMKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ

Sequences:

>Translated_227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLMKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
>Mature_227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLMKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ

Specific function: Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA in response to increasing levels of copper ions, and probably silver ions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=99.5594713656388, Blast_Score=455, Evalue=1e-130,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=51.1415525114155, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=39.9103139013453, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=34.0909090909091, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=34.841628959276, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=35.7466063348416, Blast_Score=126, Evalue=9e-31,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=33.4821428571429, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1786911, Length=220, Percent_Identity=35.4545454545455, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=34.0807174887892, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=32.15859030837, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=34.061135371179, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=30.7359307359307, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=28.7610619469027, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=33.6448598130841, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788713, Length=115, Percent_Identity=32.1739130434783, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=124, Percent_Identity=27.4193548387097, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR006291
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25427; Mature: 25427

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGW
CEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCH
DIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKPFAFAELLARVRTLLRRGA
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHCCC
AVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLEFFLRHQGEVLPRSLIASQVW
EEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHH
DMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGW
CEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCH
DIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKPFAFAELLARVRTLLRRGA
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHCCC
AVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLEFFLRHQGEVLPRSLIASQVW
EEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHH
DMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]