Click here to switch to the map view.

The map label for this gene is galS [H]

Identifier: 157156713

GI number: 157156713

Start: 2428967

End: 2430007

Strand: Reverse

Name: galS [H]

Synonym: EcE24377A_2446

Alternate gene names: 157156713

Gene position: 2430007-2428967 (Counterclockwise)

Preceding gene: 157158849

Following gene: 157157007

Centisome position: 48.8

GC content: 51.97

Gene sequence:

>1041_bases
ATGATCACCATTCGTGATGTAGCGCGTCAGGCTGGCGTCTCTGTGGCAACGGTTTCCCGGGTGCTCAATAACAGCACGCT
GGTCAGTGCCGACACGCGTGAAGCAGTAATGAAAGCCGTGAGTGAGCTGGATTATCGGCCAAATGCCAATGCCCAGGCGC
TGGCAACTCAGGTTAGCGATACCATTGGCGTGGTGGTGATGGACGTTTCTGATGCTTTTTTCGGCGCGCTGGTAAAAGCG
GTGGATCTGGTCGCCCAGCAGCATCAGAAATACGTGCTAATCGGCAATAGCTATCATGAAGCGGAAAAAGAGCGTCACGC
CATTGAGGTGTTAATTCGCCAGCGTTGTAATGCGTTGATTGTTCACTCAAAAGCATTGAGTGACGATGAACTGGCGCAAT
TTATGGATAACATTCCCGGTATGGTGTTAATCAACCGCGTTGTGCCGGGGTACGCCCATCGTTGCGTTTGCCTGGATAAT
CTCAGCGGTGCCCGAATGGCGACGCGCATGTTGCTGAATAACTGTCATCAACGTATTGGTTATCTTTCTTCCAGCCACGG
CATTGAAGATGACGCCATGCGTAAAGCAGGCTGGATGAGTGCGTTGAAAGAGCAGGATATTATTCCGCCGGAAAGCTGGA
TTGGCACTGGTACGCCGGACATGCCGGGCGGTGAGGCGGCGATGGTTGAACTGCTGGGGCGCAATCTACAACTTACCGCT
GTATTTGCTTATAACGACAATATGGCCGCTGGCGCACTGACAGCATTAAAAGATAATGGCATTGCGATTCCGTTACATCT
CTCAATCATCGGTTTCGATGATATTCCCATCGCCCGTTACACCGACCCGCAATTAACGACCGTGCGTTATCCCATTGCTT
CAATGGCGAAATTAGCCACCGAACTGGCCTTGCAGGGGGCAGCAGGCAATATTGATCCTCGTGCCAGCCACTGTTTTATG
CCGACGTTAGTGCGTCGCCATTCTGTCGCAACACGCCAGAATGCGGCGACGATCACTAACTCAACAAATCAGGCGATGTA
A

Upstream 100 bases:

>100_bases
TCCATTGCTGTGACTCGATTCACGAAGTCCTGTATTCAGTGCTGACAAAATAGCCGCCAGCAAGCAGTCATTTACTGCAA
TCTCATAACAGGTAGTGAAT

Downstream 100 bases:

>100_bases
CCGCTTTCAATCTGTGAGTGATTTCACAGTATCTTAACAATGTGATAGCTATGATTGCACCGTTTTAACGTTGTAACCCG
TATGTAACAGTGAATAATCA

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNCHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFM
PTLVRRHSVATRQNAATITNSTNQAM

Sequences:

>Translated_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNCHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFM
PTLVRRHSVATRQNAATITNSTNQAM
>Mature_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNCHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFM
PTLVRRHSVATRQNAATITNSTNQAM

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=99.1329479768786, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=55.5891238670695, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=35.3293413173653, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=34.8534201954397, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=29.5081967213115, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=29.080118694362, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=28.4375, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.5019157088123, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=25.4071661237785, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=25.9615384615385, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789456, Length=353, Percent_Identity=26.628895184136, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.4295532646048, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786268, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37463; Mature: 37463

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNCHQRIG
EECCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHC
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAATITNSTNQAM
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNCHQRIG
EECCCCCHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHC
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAATITNSTNQAM
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]