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The map label for this gene is purH [H]

Identifier: 157156680

GI number: 157156680

Start: 4542948

End: 4544537

Strand: Reverse

Name: purH [H]

Synonym: EcE24377A_4550

Alternate gene names: 157156680

Gene position: 4544537-4542948 (Counterclockwise)

Preceding gene: 157157102

Following gene: 157156020

Centisome position: 91.26

GC content: 55.97

Gene sequence:

>1590_bases
ATGCAACAACGTCGTCCAATCCGCCGCGCTCTGCTCAGTGTTTCTGACAAAGCCGGTATCGTCGAATTCGCCCAGGCACT
TTCCGCACGCGGTGTGGAGCTGCTGTCTACAGGGGGCACTGCCCGTCTGTTAGCAGAAAAAGGTCTGCCGGTAACCGAAG
TTTCCGATTACACCGGTTTCCCGGAGATGATGGATGGACGCGTGAAGACCCTGCATCCGAAAGTACATGGTGGCATTCTG
GGCCGTCGCGGCCAGGACGATGCCATTATGGAAGAACATCAGATCCAGCCTATCGATATGGTGGTTGTTAACCTGTATCC
GTTCGCCAAGACCGTTGCCCGTGAAGGTTGCTCGCTGGAAGATGCGGTTGAGAACATCGATATCGGCGGCCCAACGATGG
TGCGCTCCGCCGCCAAGAACCATAAAGATGTCGCAATCGTGGTGAAGAGCAGCGACTACGACGCCATTATTAAAGAGATG
GATGACAACGAAGGATCGCTGACGCTTGCAACCCGTTTCGACCTCGCCATCAAAGCCTTCGAACACACTGCCGCCTACGA
CAGCATGATTGCCAACTACTTCGGCAGCATGGTTCCGGCTTACCACGGTGAAAGCAAAGAAGCCGCCGGTCGCTTCCCAC
GCACGCTGAACCTGAACTTCATTAAGAAGCAGGATATGCGTTACGGCGAGAACAGCCACCAGCAGGCTGCCTTCTATATA
GAAGAGAATGTGAAAGAAGCCTCCGTTGCTACCGCAACCCAGGTTCAGGGTAAAGCCCTCTCTTATAACAACATCGCCGA
TACCGATGCGGCGCTGGAGTGCGTGAAAGAGTTCGCCGAGCCGGCATGTGTGATTGTGAAGCACGCCAACCCTTGCGGCG
TGGCTATCAGCAATTCTATTCTTGATGCTTACGATCGCGCGTACAAAACCGACCCAACCTCCGCATTCGGCGGCATCATT
GCCTTTAACCGCGAGCTGGATGCGGAAACCGCACAGGCCATCATTTCTCGTCAGTTTGTCGAAGTGATTATTGCGCCTTC
CGCCAGCGAAGAAGCCCTGAAAATCACCGCCGCCAAGCAGAACGTACGCGTTCTGACCTGCGGTCAGTGGGGCGAGCGTG
TTCCGGGTCTTGATTTCAAACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGTGACCTGGGCATGGTCGGTGCGGAAGAA
CTGCGCGTCGTCACCCAACGTCAGCCGACCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAAGTGGCGAAGTTCGT
GAAATCCAACGCTATCGTCTATGCCAAAAACAATATGACCATCGGGATTGGCGCGGGCCAGATGAGCCGCGTGTACTCCG
CGAAAATCGCCGGTATTAAAGCTGCCGATGAAGGCCTGGAAGTGAAAGGTTCCTCGATGGCTTCTGACGCGTTCTTCCCG
TTCCGCGACGGTATTGATGCCGCCGCCGCTGCAGGCGTGACCTGCGTAATCCAGCCTGGCGGTTCTATCCGTGATGACGA
AGTGATTGCCGCCGCCGACGAGCACGGTATTGCGATGCTCTTCACCGACATGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
CGGGCGAAAAATAAGGATGCCCCGTTAGGGGCGTTAGCTGAGTTTTTCGCGAAAAATTCAGCTAACGCTCTCTGTAATAG
TCAAATCCAGGGGATTTACC

Downstream 100 bases:

>100_bases
TGGAGCAATAGATGAAAGTATTAGTGATTGGTAACGGCGGGCGCGAGCACGCGCTGGCCTGGAAAGCGGCGCAGTCGCCG
CTGGTTGAAACTGTTTTTGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH

Sequences:

>Translated_529_residues
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
>Mature_529_residues
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=39.3814432989691, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=98.8657844990548, Blast_Score=1082, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=33.7133550488599, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71985574, Length=354, Percent_Identity=27.1186440677966, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=141, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=491, Percent_Identity=35.4378818737271, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=35.8762886597938, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=34.5335515548282, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57403; Mature: 57403

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDDNEGSLTLATRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSI
ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTQRQPTEQELRDALFC
CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MQQRRPIRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVVVNLYPFAKTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDDNEGSLTLATRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAISNSI
ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTQRQPTEQELRDALFC
CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA