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The map label for this gene is yeiM [H]

Identifier: 157156640

GI number: 157156640

Start: 2444373

End: 2445623

Strand: Reverse

Name: yeiM [H]

Synonym: EcE24377A_2461

Alternate gene names: 157156640

Gene position: 2445623-2444373 (Counterclockwise)

Preceding gene: 157155991

Following gene: 157155576

Centisome position: 49.11

GC content: 49.48

Gene sequence:

>1251_bases
ATGGATATAATGAGAAGTGTTGTGGGGATGGTGGTGTTACTGGCAATAGCATTTCTGTTGTCAGTGAATAAAAAGAGCAT
CAGTTTGCGCACGGTTGGAGCCGCACTGCTGCTGCAAATCGCTATTGGTGGCATCATGCTCTACTTCCCACCGGGAAAGT
GGGCAGTAGAACAGGCGGCATTAGGCGTTCATAAAGTGATGTCTTACAGTGATGCCGGTAGCGCCTTCATTTTTGGTTCG
CTGGTTGGGCCGAAAATGGATGTCCTGTTTGACGGTGCGGGTTTTATCTTCGCCTTTCGCGTACTTCCGGCGATTATTTT
CGTTACTGCGCTCATCAGTCTGCTGTACTACATTGGCGTGATGGGGCTGCTGATTCGTATTCTTGGCAGTATTTTTCAGA
AAGCCCTCAACATCAGCAAAATCGAATCTTTTGTCGCAGTCACCACTATTTTCCTCGGGCAAAATGAGATCCCGGCGATC
GTTAAACCTTTTATTGATCGCATGAATCGCAACGAGCTATTTACGGCTATTTGTAGCGGCATGGCGTCTATTGCGGGTTC
GATGATGATTGGCTATGCCGGAATGGGCGTGCCGATTGACTATTTGTTAGCCGCTTCGCTGATGGCTATTCCTGGCGGTA
TCCTGTTTGCACGTATTCTTAGTCCGGCGACGGAACCTTCGCAAGTTACGTTTGAAAATCTGTCGTTTAGCGAAACACCG
CCAAAAAGCATTATTGAAGCTGCGGCGAACGGTGCAATGACCGGGCTGAAAATCGCCGCCGGTGTGGCGACGGTGGTAAT
GGCATTTGTGGCAATTATTGCGCTGATCAATGGCATTATTGGTGGAGTTGGCGGCTGGTTTGGTTTTGCCAATGTCTCTC
TGGAAAGCATTTTCGGCTATGTGCTGGCCCCGTTGGCATGGATTATGGGGGTGGACTGGAGTGATGCAAATCTTGCCGGA
AGTTTGATTGGGCAGAAACTGGCGATTAATGAGTTTGTTGCTTATCTCAATTTCTCGCCCTATCTGCAAACGAGCGGCAC
GCTGGATGTCAAGACCATTGCCATTATCTCGTTCGCCCTCTGTGGGTTTGCAAACTTTGGTTCTATCGGTGTTGTCGTTG
GCGCATTTTCGGCTATTTCGCCAAAACGTGCTCCAGAAATCGCCCAGCTTGGTTTACGGGCGCTGGCAGCAGCAACGCTT
TCCAACCTGATGAGTGCGACTATTGCCGGGTTCTTTATTGGTCTGGCGTAA

Upstream 100 bases:

>100_bases
GGGTTAATTTCTGACGGGCAGAAAGCCGTCGCCCGTACTCATCAAGCATACCAAAACCTGGCGCTGACGCCGGGTTTACT
GGCATGAAGGGAAAATCATT

Downstream 100 bases:

>100_bases
TGCTCGATTTGTAGACCGGATAAAACGTATTAAGCGTTGCATCCGGCAATCGCGCTATTGCAAATAATTTTTACTGCATC
ATCCCGGAGAATTTATTCTC

Product: NupC family nucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA

Sequences:

>Translated_416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA
>Mature_416_residues
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGS
LVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
PKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAG
SLIGQKLAINEFVAYLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATL
SNLMSATIAGFFIGLA

Specific function: Could be involved in pseudouridine transport [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI11545853, Length=415, Percent_Identity=32.7710843373494, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI42542381, Length=418, Percent_Identity=31.8181818181818, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI227116277, Length=419, Percent_Identity=30.3102625298329, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1788488, Length=416, Percent_Identity=98.3173076923077, Blast_Score=798, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=416, Percent_Identity=88.9423076923077, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1788737, Length=412, Percent_Identity=30.5825242718447, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17560276, Length=396, Percent_Identity=27.7777777777778, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI71991794, Length=399, Percent_Identity=30.5764411027569, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25146537, Length=194, Percent_Identity=28.8659793814433, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45552519, Length=408, Percent_Identity=30.6372549019608, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45552517, Length=408, Percent_Identity=30.6372549019608, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI19921868, Length=408, Percent_Identity=30.6372549019608, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI281360430, Length=407, Percent_Identity=31.2039312039312, Blast_Score=201, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011642
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07670 Gate; PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43482; Mature: 43482

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
LGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGV
HHHHHHHHCCCCCCCEEEHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
MASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFANVSLESIFGY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVAYLNFSPYLQTSGTLDVKTIAIISFAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
CGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA
HCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGGIMLYFPPGKWAVEQAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
LGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGV
HHHHHHHHCCCCCCCEEEHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
MASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCCC
PKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFANVSLESIFGY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVAYLNFSPYLQTSGTLDVKTIAIISFAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
CGFANFGSIGVVVGAFSAISPKRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA
HCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]