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The map label for this gene is dcuA [H]

Identifier: 157156434

GI number: 157156434

Start: 4696874

End: 4698175

Strand: Reverse

Name: dcuA [H]

Synonym: EcE24377A_4693

Alternate gene names: 157156434

Gene position: 4698175-4696874 (Counterclockwise)

Preceding gene: 157157079

Following gene: 157157801

Centisome position: 94.35

GC content: 52.3

Gene sequence:

>1302_bases
ATGCTAGTTGTAGAACTCATCATAGTTTTGCTGGCGATCTTCTTGGGCGCCAGATTGGGGGGAATAGGTATTGGTTTTGC
AGGCGGATTGGGGGTGCTGGTTCTTGCCGCTATTGGCGTTAAACCCGGTAACATCCCGTTCGATGTCATTTCCATTATCA
TGGCGGTTATCGCCGCTATTTCTGCCATGCAGGTTGCTGGCGGTCTGGACTATCTGGTTCATCAGACAGAAAAGCTGCTG
CGCCGTAACCCGAAATACATCACGATCCTCGCACCGATCGTGACCTATTTCCTGACTATCTTTGCTGGTACTGGCAACAT
ATCTCTGGCGACACTGCCAGTTATCGCTGAAGTTGCGAAGGAACAAGGCGTCAAACCTTGCCGTCCGCTGTCTACTGCAG
TGGTATCCGCGCAGATTGCGATCACCGCATCGCCAATCTCAGCGGCAGTGGTTTACATGTCTTCCGTGATGGAAGGTCAT
GGCATCAGCTACCTCCATCTGCTCTCCGTGGTCATCCCGTCCACCCTGCTGGCGGTTCTGGTGATGTCCTTTCTGGTCAC
TATGCTGTTCAACTCCAAACTCTCTGACGATCCGATTTATCGCAAGCGTCTGGAAGAGGGCCTGGTTGAACTGCGCGGTG
AAAAGCAGATTGAAATCAAATCCGGTGCAAAAACGTCCGTCTGGCTGTTCCTGCTGGGCGTAGTTGGCGTGGTTATCTAT
GCAATCATCAACAGCCCAAGCATGGGTCTGGTTGAAAAACCGCTGATGAACACCACCAACGCAATCCTGATCATCATGCT
CAGCGTTGCAACTCTGACCACCGTTATCTGTAAAGTCGATACCGACAACATCCTCAACTCCAGCACCTTCAAAGCAGGTA
TGAGCGCCTGTATTTGTATCCTGGGTGTTGCGTGGCTGGGCGATACTTTCGTTTCCAACAACATCGACTGGATCAAAGAT
ACCGCTGGTGAAGTGATTCAGGGTCATCCGTGGCTGCTGGCCGTCATCTTCTTCTTTGCTTCTGCTCTGCTGTACTCTCA
GGCTGCAACCGCAAAAGCACTAATGCCGATGGCTCTGGCACTGAACGTTTCACCGCTGACCGCTGTTGCTTCTTTCGCTG
CGGTGTCTGGTCTGTTCATTCTGCCGACCTACCCGACGCTGGTTGCTGCGGTACAGATGGATGACACGGGTACTACCCGT
ATCGGTAAATTCGTCTTCAACCATCCGTTCTTCATCCCGGGTACTCTGGGTGTTGCCCTGGCCGTTTGCTTCGGCTTCTT
GCTGGGTAGCTTCATGCTGTAA

Upstream 100 bases:

>100_bases
AATAAAGAAGGCACGTCAGATGACGTGCCTTTTTTCTTGTGAGCAGTAACTTAAAAATAACAACCTAATATCAACTTGTT
AAAAAACAAGGAAGGCTAAT

Downstream 100 bases:

>100_bases
TGACCAATCGCGGGGCGTTCACGCCCCGCTTTCTTTCCCGCCGACTAACATCCTTCCCCCGTCCGTTGTATAGTGACCTC
TCTCTTGCGGTTCCATCTGT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML

Sequences:

>Translated_433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML
>Mature_433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=433, Percent_Identity=99.7690531177829, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI1790565, Length=404, Percent_Identity=36.1386138613861, Blast_Score=241, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45765; Mature: 45765

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYLHLLSVVIPSTLLAVL
HCCCCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVAL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AVCFGFLLGSFML
HHHHHHHHHHHCC
>Mature Secondary Structure
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYLHLLSVVIPSTLLAVL
HCCCCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVAL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AVCFGFLLGSFML
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]