Click here to switch to the map view.

The map label for this gene is alkB [H]

Identifier: 157156371

GI number: 157156371

Start: 2495040

End: 2495690

Strand: Reverse

Name: alkB [H]

Synonym: EcE24377A_2511

Alternate gene names: 157156371

Gene position: 2495690-2495040 (Counterclockwise)

Preceding gene: 157156573

Following gene: 157157028

Centisome position: 50.12

GC content: 52.53

Gene sequence:

>651_bases
ATGTTGGATCTGTTTGCCGATGCTGAACCGTGGCAAGAGCCACTGGCGGCTGGAGCGGTAATTTTGCACCGTTTTGCTTT
TAACGCTGCGGAGCAACTGATCCGCGATATTAATAACGTTGCCAGCCAGTCGCCGTTTCGCCAGATGGTCACCCCCGGGG
GATATACCATGTCGGTGGCGATGACCAACTGTGGGCATCTGGGCTGGACGAGCCATCGGCAAGGTTATCTCTATTCGCCC
ATTGATCCGCAAACAAATAAACCGTGGCCCGCCATGCCACAGAGTTTTCATAATTTATGTCAACGTGCGGCTACGGCGGC
GGGCTATCCAGATTTCCAGCCAGATGCTTGTCTTATCAACCGCTACGCTCCTGGCGCGAAACTGTCGCTGCATCAGGATA
AAGACGAACCGGATCTGCGCGCGCCAATTGTTTCTGTTTCTCTGGGCTTACCCGCGATTTTTCAATTTGGTGGCCTGAAA
CGTAATGATCCGCTCAAACGTTTGTTGTTGGAACATGGCGATGTGGTGGTATGGGGCGGTGAATCGCGGCTGTTTTATCA
CGGTATTCAACCGTTGAAAGCGGGGTTTCATCCACTCACCACCGACTGCCGCTACAACCTGACATTCCGTCAGGCAGGTA
AAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCGGGTGGTCCGTGGTGATGGCACACTTTCCGGTTACCGCTGGGGCGTGTCGCGTAAAGCGCAACTGCTGCGCCGCGAA
GCTGAAAATGAGGAGAGGTA

Downstream 100 bases:

>100_bases
AAATAAGAATTATTATTGCTGTGCGCGCGAAGATGTTTAAACTGCGGGCTGTAATTCATTGTCCGGGTTTTCTGCATGGA
ACTTCTTGTACTTGTCTGGC

Product: alkylated DNA repair protein AlkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSP
IDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE

Sequences:

>Translated_216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSP
IDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE
>Mature_216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSP
IDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=98, Percent_Identity=33.6734693877551, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=98.1481481481482, Blast_Score=445, Evalue=1e-127,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=41.8367346938776, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24030; Mature: 24030

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE
CCEEEECCCCHHHCCCCCCCCCCEEEEEHHCCCCCC
>Mature Secondary Structure
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE
CCEEEECCCCHHHCCCCCCCCCCEEEEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]