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The map label for this gene is hypF [H]

Identifier: 157156292

GI number: 157156292

Start: 2995842

End: 2998094

Strand: Reverse

Name: hypF [H]

Synonym: EcE24377A_2996

Alternate gene names: 157156292

Gene position: 2998094-2995842 (Counterclockwise)

Preceding gene: 157155890

Following gene: 157157873

Centisome position: 60.21

GC content: 58.06

Gene sequence:

>2253_bases
ATGGCAAAAAACACATCTTGCGGTGTCCAACTGCGTATTCGAGGCAAAGTGCAGGGCGTCGGTTTTCGTCCGTTTGTCTG
GCAGCTGGCACAGCAATTAAATCTTCACGGCGATGTCTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCGGGAAGACC
CGGAAACGTTTCTTGTTCAATTGCATCAGCACTGCCCGCCGCTGGCGCGGATTGATAGCGTCGAGCGTGAGCCGTACATC
TGGTCACAACTGCCCACTGAGTTCACCATCCGCCAGAGCGCGGGAGGTGCCATGAATACGCAAATTGTCCCGGATGCCGC
TACTTGCCCTGCTTGCCTTGCCGAAATGAATACCCCAGGCGAACGGCGTTATCGTTATCCGTTTATCAACTGTACCCACT
GCGGCCCGCGTTTCACCATTATTCGCGCCATGCCTTACGACCGCCCGTTTACCGTGATGGCGGCGTTTCCGTTGTGTCCG
GCTTGTGACAAAGAGTACCGCGACCCGCTCGATCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCGCA
TCTTGAATGGGTAAGTCATGGTGAACATGCAGAACAAGAGGCGGCATTACAGGCGGCTATCGCACAGTTAAAAATGGGCA
ACATTGTCGCCATCAAAGGGATTGGCGGATTTCATCTTGCCTGCGATGCACGTAACAGTAACGCGGTGGCGACACTTCGG
GCGCGCAAACATCGCCCGGCGAAACCGCTGGCGGTCATGTTGCCAGTGGCTGACGGTTTACCAGACGCTGCGCGCCAGTT
GCTTACCACGCCCGCCGCGCCGATTGTGCTGGTGGATAAAAAATACGTTCCTGAGCTTTGTGATGATATCGCCCCTGACC
TTAACGAAGTCGGGGTAATGTTGCCTGCGAACCCGCTCCAGCATTTGCTGTTACAGGAACTGCAATGCCCGCTGGTGATG
ACCTCCGGCAACCTGAGCGGTAAACCACCAGCTATCAGCAACGAACAGGCGCTGGCGGATTTGCAGGGCATTGCCGACGG
ATTCTTGATACATAACCGCGACATCGTGCAGCGGATGGATGATTCGGTGGTGCGCGAAAGCGGCGAAATGCTGCGCCGTT
CGCGGGGGTATGTGCCGGATGCGCTGGCTTTGCCTCCGGGCTTTAAAAATGTTCCGCCTGTGCTGTGTCTCGGCGCGGAT
CTGAAAAATACCTTCTGCCTGGTGCGCGGTGAACAAGCGGTGTTGAGTCAGCATCTGGGCGATTTAAGTGACGATGGCAT
CCAGATGCAGTGGCGCGAAGCGTTACGCCTGATGCAAAACATCTACGATTTTACCCCGCAATACGTTGTGCATGACGCGC
ATCCGGGCTATGTCTCCAGCCAGTGGGCGCGTGAAATGAATCTGCCGACGCAAACGGTACTGCATCATCATGCCCATGCA
GCGGCGTGTCTGGCAGAGCATCAGTGGCCGCTGGATGGCGGTGATGTCATTGCTTTGACGCTCGACGGTATCGGTATGGG
GGAGAACGGCGCTTTGTGGGGCGGCGAGTGCCTGCGGGTGAACTATCGCGAATGTGAGCACCTGGGCGGCTTGCCTGCAG
TGGCGCTTCCGGGTGGCGATTTGGCAGCGAAGCAGCCGTGGCGAAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAG
TGGCAGAATTACCCTGAAACAGCAAGTGTGCAACAGCAAAACTGGAGCGTGCTGGCGCGGGCCATTGAGCGTGGAATTAA
CGCGCCGCTGGCGTCATCGTGTGGGCGGTTGTTCGATGCAGTGGCGGCGGCACTGGGCTGTGCGCCAGCCACGTTAAGTT
ATGAAGGTGAAGCGGCTTGTGCTCTGGAGGCGCTCGCAGCCTCATGCCACGGAGTGACGCATCCGGTGACGATGCCGCTG
GTGGACAATCAACTGGATCTCGCCACTTTCTGGCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGC
GTTTCATGATGCGCTGGCGCAGGGTTTTGCCGCGTTGATGCGTGAGCAGGCCACGATGCGTGGTATCACTACGCTGGTAT
TTAGCGGCGGGGTTATTCATAACCGTTTGCTGCGTGCACGTCTGGCGCATTATCTCGCTGATTTCACATTGCTCTTTCCA
CAGAGTTTACCGGCGGGTGATGGCGGTTTGTCTCTGGGGCAGGGGGTTATTGCTGCGGCGCGTTGGTTAGCGGGTGAAGT
CCAGAACGGATAA

Upstream 100 bases:

>100_bases
AATACGGAGCTGGCGGTATCGCCGACCTGAGACTGCCGGATACGACGCAGTCGTGTCCGGCAGGGCGGGTTACGCAATTC
ATGATGGCGGGAATAACTCA

Downstream 100 bases:

>100_bases
AACGTGTTGCATTTGGACACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACACATGCGTCCCCGTAGGTCGGA
TAAGACGCGCCAGCGTCGCA

Product: carbamoyltransferase HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYI
WSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
ARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPL
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG

Sequences:

>Translated_750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYI
WSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
ARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPL
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
>Mature_749_residues
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIW
SQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPA
CDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLRA
RKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMT
SGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADL
KNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHAA
ACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEW
QNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPLV
DNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQ
SLPAGDGGLSLGQGVIAAARWLAGEVQNG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=750, Percent_Identity=98.9333333333333, Blast_Score=1525, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81916; Mature: 81785

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLREDPETFLVQ
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHH
LHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPG
HHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEE
ARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPDLNEVGVM
HHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECEECCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLREDPETFLVQ
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHH
LHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPG
HHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEE
ARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPDLNEVGVM
HHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECEECCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]