Click here to switch to the map view.

The map label for this gene is ycjW [H]

Identifier: 157156207

GI number: 157156207

Start: 1514566

End: 1515564

Strand: Reverse

Name: ycjW [H]

Synonym: EcE24377A_1531

Alternate gene names: 157156207

Gene position: 1515564-1514566 (Counterclockwise)

Preceding gene: 157155990

Following gene: 157156103

Centisome position: 30.44

GC content: 45.15

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
TAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAGCCAAACAAGCTGGCCCGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCTGAAGTTTTGCATGCCATCACCGCCAAAGCTGAAGAGGAAGGTTTCGACGTGATATTGCAGACGTCGCACAATCCGGC
AGAAGACTTACAAAAATGTGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTAAGTTCGCCAGCGGATG
AGTCATTTTTTGCCCAACTTGATAAATACGATATTCCTGTCGTGGTGATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGCGACAGCATTGCGTTGACCGATGCATTAATTGAAAGTGGGCATCAAAATATTGC
CTGCCTGCATGCTCCGCTTGATGTCCATGTTTCAGTGGATCGGGTAAATGGTTATAAGCAGAGCCTGGCTGCGCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGACGGCGGTTATACCCATGAAACAGCACTGCAAGCCGCGCGGGAATTATTAAGC
CAGTCACCGTTGCCTGAGGCAGTGTTTGCTACTGACAGCCTGAAATTAATGAGTATTTATCGTGCGGCAGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGTAATGAAACGCTGTCGTTTATTTTAACGCCTGCGCCAGGCG
GTATTGATATCCCAACACAGGAGTTAGGTCAACAAAGCTGTGAGTTATTATTCCGCTTAATTTCTGGAAAACCGTCACCA
CAGAATATTACCGTTGCCACGCATATGACGCTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTGCCCGTGTTGTCATCGACGGGCGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATACCAGCTACCTTGCCTGAATGCCGGATGCGATGCTAGCGCATCTTATCCGGCCTACATTTATAGTTCGGATAAAGTAC
TCGTGCCGCATCCGGCAATT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQ
NITVATHMTLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=99.0963855421687, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.5555555555556, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=30.0940438871473, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.4117647058824, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=26.8987341772152, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=29.0625, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.2365930599369, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=25.3048780487805, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=27.8787878787879, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=21.25, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36150; Mature: 36018

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDIPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDIPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]