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The map label for this gene is nanE

Identifier: 157156142

GI number: 157156142

Start: 3700477

End: 3701166

Strand: Reverse

Name: nanE

Synonym: EcE24377A_3705

Alternate gene names: 157156142

Gene position: 3701166-3700477 (Counterclockwise)

Preceding gene: 157157675

Following gene: 157155309

Centisome position: 74.33

GC content: 57.83

Gene sequence:

>690_bases
ATGTCGTTACTTGCACAACTGGATCAAAAAATCGCTGCTAACGGTGGCCTGATTGTCTCCTGCCAGCCGGTTCCGGACAG
CCCGCTCGATAAACCCGAAATCGTCGCCGCCATGGCATTAGCGGCAGAACAGGCGGGCGCGGTTGCCATTCGCATTGAAG
GTGTGGCAAATCAGCAAGCCACGCGTGCGGTGGTGAGCGTGCCGATTATTGGAATTGTGAAACGCGATCTGGAGGATTCT
CCGGTACGCATCACGGCCTATATTGAAGATGTTGATGCGCTGGCGCAGGCGGGCGCGGACATTATCGCCATTGACGGCAC
CGACCGCCCGCGTCCGGTGCCTGTTGAAACGCTGCTGGCACGTATTCACCATCACGGTTTACTGGCGATGACCGACTGCT
CAACGCCGGAAGACGGCCTGGCATGCCAAAAGCTGGGAGCCGAAATTATTGGCACTACGCTTTCTGGCTATACCACGCCT
GAAACGCCAGAAGAGCCGGATCTGGCGCTGGTGAAAACGTTGAGCGACGCCGGATGTAGGGTGATTGCCGAAGGGCGTTA
CAACACGCCTGCTCAGGCGGCAGATGCGATGCGCCACGGCGCGTGGGCGGTGACGGTCGGTTCTGCCATTACGCGTCTTG
AACACATTTGTCAGTGGTACAACACAGCGATGAAAAAGGCGGTGCTATGA

Upstream 100 bases:

>100_bases
GTGCCGTGCCATTTGGCAGCGCCAAAAACGATTTAGTCAAAACCAAAAGTTAATCCTATTGCCCGGTCTATGTACCGGGC
CTTTCGCTAAGGGAAGATGT

Downstream 100 bases:

>100_bases
CCACGCTGGCGATTGATATCGGCGGTACTAAACTTGCCGCCGCGCTGATTGGCGCTGACGGGCAGATCCGCGATCGTCGT
GAACTTCCTACGCCAGCCAG

Product: N-acetylmannosamine-6-phosphate 2-epimerase

Products: NA

Alternate protein names: ManNAc-6-P epimerase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQAGAVAIRIEGVANQQATRAVVSVPIIGIVKRDLEDS
PVRITAYIEDVDALAQAGADIIAIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL

Sequences:

>Translated_229_residues
MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQAGAVAIRIEGVANQQATRAVVSVPIIGIVKRDLEDS
PVRITAYIEDVDALAQAGADIIAIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL
>Mature_228_residues
SLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQAGAVAIRIEGVANQQATRAVVSVPIIGIVKRDLEDSP
VRITAYIEDVDALAQAGADIIAIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTPE
TPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential)

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family

Homologues:

Organism=Escherichia coli, GI1789617, Length=229, Percent_Identity=99.5633187772926, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANE_ECO24 (A7ZSB6)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464695.1
- ProteinModelPortal:   A7ZSB6
- SMR:   A7ZSB6
- STRING:   A7ZSB6
- EnsemblBacteria:   EBESCT00000019619
- GeneID:   5585959
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_3705
- NMPDR:   fig|331111.3.peg.1375
- eggNOG:   COG3010
- GeneTree:   EBGT00050000010584
- HOGENOM:   HBG296941
- OMA:   EHIVSWF
- ProtClustDB:   PRK01130
- BioCyc:   ECOL331111:ECE24377A_3705-MONOMER
- HAMAP:   MF_01235
- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF04131 NanE; SSF51366 RibP_bind_barrel

EC number: =5.1.3.9

Molecular weight: Translated: 24089; Mature: 23958

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQAGAVAIRIEGVANQQA
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHH
TRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADIIAIDGTDRPRPVPVETLLA
HHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
RIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTPETPEEPDLALVKTLSDAGCR
HHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCE
VIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL
EEECCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQAGAVAIRIEGVANQQA
CHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHH
TRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADIIAIDGTDRPRPVPVETLLA
HHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
RIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTPETPEEPDLALVKTLSDAGCR
HHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCE
VIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL
EEECCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA