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The map label for this gene is cysN [H]

Identifier: 157156140

GI number: 157156140

Start: 3052762

End: 3054189

Strand: Reverse

Name: cysN [H]

Synonym: EcE24377A_3052

Alternate gene names: 157156140

Gene position: 3054189-3052762 (Counterclockwise)

Preceding gene: 157158131

Following gene: 157155510

Centisome position: 61.33

GC content: 54.62

Gene sequence:

>1428_bases
ATGAACACCGCACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGCCTGCTGCACGATACCCGCCAAA
TCTACGAAGATCAGCTCTCATCGCTGCATAACGACAGTAAGCGTCACGGCACCCAGGGCGAAAAACTGGATCTGGCTCTG
CTGGTGGACGGCCTGCAAGCTGAGCGCGAACAGGGCATCACTATTGATGTGGCCTACCGCTATTTCTCTACCGAGAAGCG
TAAATTTATTATCGCCGACACCCCCGGGCACGAGCAGTATACCCGCAATATGGCGACCGGCGCCTCAACGTGTGAGCTGG
CGATCTTACTGATCGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACGCTGCTGGGGATC
AAACATCTGGTCGTAGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACATTCACCCGCATTCGTAAAGACTA
CCTGACTTTCGCCGAACAGTTACCTGGCAATCTGGATATTCGCTTTGTGCCGCTCTCTGCGCTGGAAGGCGACAACGTGG
CTTCGCAAAGTGAAAGTATGCCGTGGTACAGCGGTCCGACACTGCTCGAAGTGCTGGAAACCGTGGAGATCCAGCGAGTG
GTGGATGCTCAGCCAATGCGCTTCCCGGTGCAGTACGTTAATCGCCCGAATCTCGATTTTCGTGGCTACGCCGGAACGCT
GGCATCCGGTCGCGTGGAAGTCGGGCAACGTGTAAAAGTGCTGCCTTCTGGTGTGGAATCAAACGTCGCGCGGATCGTGA
CTTTTGATGGTGATCGCGAAGAAGCCTTTGCCGGAGAAGCGATCACCCTGGTGCTGACGGATGAGATCGACATCAGCCGT
GGCGATCTGCTGCTGGCGGCAGACGAAGCGTTACCGGCGGTGCAGAGCGCGTCGGTGGATGTGGTATGGATGGCGGAACA
GCCGCTTTCTCCAGGGCAGAGTTACGACATCAAAATTGCCGGTAAGAAGACGCGCGCGCGTGTTGATGGCATTCGCTATC
AGGTTGATATTAATAACCTTACCCAGCGTGAAGTTGAAAACCTGCCACTGAATGGGATCGGCCTCGTGGATCTCACTTTT
GACGAGCCGCTGGTGTTAGATCGTTATCAACAAAATCCGGTGACGGGTGGGCTGATTTTTATCGATCGCCTGAGCAATGT
GACCGTGGGTGCCGGTATGGTGCACGAGCCAGTTAGCCAGGCAACTGCTGCGCCATCTGAATTCAGTGCATTCGAACTGG
AATTGAATGCTCTGGTTCGTCGCCACTTTCCGCACTGGGGCGCGCGCGATTTGCTGGGGGATAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCCACCACCAGTGAACGTCAGGGCCGCGTGATTGACCGCGACCAGGCGGGGTCTATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGCCATCCGGTCACTGTGCAACAACGCGAGCTACACCACGGTCATCGT
GGTGTAGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGDK

Sequences:

>Translated_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGDK
>Mature_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGDK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=28.735632183908, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=27.6643990929705, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=28.735632183908, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=26.984126984127, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=33.7883959044369, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=33.7883959044369, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=33.7883959044369, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=32.7645051194539, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI34147630, Length=305, Percent_Identity=29.1803278688525, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=99.5789473684211, Blast_Score=968, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=324, Percent_Identity=26.8518518518519, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789737, Length=324, Percent_Identity=26.8518518518519, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI2367247, Length=296, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532067, Length=342, Percent_Identity=29.5321637426901, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=342, Percent_Identity=29.5321637426901, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=29.2134831460674, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=29.2134831460674, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.4037558685446, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.8538812785388, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=26.9709543568465, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=28.448275862069, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=391, Percent_Identity=29.6675191815857, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=28.1179138321995, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=28.1179138321995, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=28.1464530892449, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=192, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=28.604118993135, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553807, Length=276, Percent_Identity=33.695652173913, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI45553816, Length=276, Percent_Identity=33.695652173913, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24651721, Length=276, Percent_Identity=33.695652173913, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17864154, Length=276, Percent_Identity=33.695652173913, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=26.530612244898, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=26.530612244898, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=30.7936507936508, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281363316, Length=312, Percent_Identity=28.2051282051282, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17864358, Length=312, Percent_Identity=28.2051282051282, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52630; Mature: 52630

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAPSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA