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The map label for this gene is prmB [H]

Identifier: 157155888

GI number: 157155888

Start: 2617986

End: 2618918

Strand: Reverse

Name: prmB [H]

Synonym: EcE24377A_2625

Alternate gene names: 157155888

Gene position: 2618918-2617986 (Counterclockwise)

Preceding gene: 157158957

Following gene: 157155097

Centisome position: 52.59

GC content: 53.16

Gene sequence:

>933_bases
GTGGATAAAATTTTCGTTGATGAAGCAGTAAATGAGCTGCAAACCATTCAGGACATGTTGCGCTGGTCGGTGAGCCGCTT
CAGCGCGGCAAATATCTGGTACGGTCACGGCACCGATAACCCGTGGGATGAAGCCGTACAGCTGGTGTTACCATCGCTCT
ACCTGCCGCTGGATATCCCGGAAGATATGCGCACCGCGCGTCTGACCTCCAGCGAAAAACATCGTATTGTTGAACGCGTG
ATCCGCCGCGTCAACGAGCGCATTCCGGTGGCTTACCTGACCAACAAAGCCTGGTTCTGCGGTCATGAATTTTACGTCGA
TGAACGCGTGCTGGTGCCGCGTTCGCCGATTGGTGAACTGATCAACAATAAATTTGCCGGACTTATCAGCAAGCAACCGC
AGCATATTTTAGATATGTGTACCGGTAGCGGCTGCATCGCCATTGCCTGTGCTTATGCCTTCCCGGAAGCGGAAGTCGAC
GCGGTAGATATCTCTCCCGACGCGCTGGCGGTTGCCGAACAGAACATCGAAGAACACGGTCTGATCCACAACGTCATTCC
GATTCGTTCCGATCTGTTCCGTGACTTGCCGAAAGTGCAGTACGACCTGATTGTCACTAACCCGCCGTACGTCGATGCGG
AAGATATGTCCGACCTGCCAAACGAATACCGTCACGAGCCGGAACTGGGCCTGGCATCTGGCACCGACGGCCTGAAACTG
ACGCGTCGCATTCTTGGAAACGCGGCAGATTACCTTGCTGATGATGGCGTGTTGATTTGTGAAGTCGGCAACAGCATGGT
ACATCTTATGGAACAATATCCGGATGTTCCGTTCACCTGGCTGGAGTTTGATAACGGCGGCGATGGTGTGTTTATGCTCA
CCAAAGAGCAGCTGCTGGCGGCCCGTGAACATTTCGCGATTTATAAAGATTAA

Upstream 100 bases:

>100_bases
ACGTATTTTTCCTCACTCACCGCGCGTGAATGCTGATTTATCGTCATCTTCATGGCAAACTAGCCGCCGAAATTATTGCG
ATCATGCCCTGGAGGAATAC

Downstream 100 bases:

>100_bases
AATTACTCGTCATTCTTCAAGTTGCATGTGCTGCGTCTGCGCTCGTTCACCCCAGTCACTTACTTCTGTAAGCTCCTGGG
GATTCACTCACTTGTCGCCT

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD

Sequences:

>Translated_310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD
>Mature_310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD

Specific function: Unknown

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=231, Percent_Identity=29.004329004329, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI87082085, Length=310, Percent_Identity=99.3548387096774, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1787463, Length=194, Percent_Identity=32.4742268041237, Blast_Score=100, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24582226, Length=199, Percent_Identity=27.6381909547739, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR017127
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35016; Mature: 35016

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
EDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGEL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEECCEEECCCCHHHHH
INNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVDAVDISPDALAVAEQNIEEHG
HHHHHHHHHCCCHHHHHHHHCCCCHHEEEEHHHCCCCCCEEEECCCCHHHHHHHCHHHCC
LIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCHHHH
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLLA
HHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHH
AREHFAIYKD
HHHHHEEECC
>Mature Secondary Structure
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
EDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGEL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEECCEEECCCCHHHHH
INNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVDAVDISPDALAVAEQNIEEHG
HHHHHHHHHCCCHHHHHHHHCCCCHHEEEEHHHCCCCCCEEEECCCCHHHHHHHCHHHCC
LIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCHHHH
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLLA
HHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHH
AREHFAIYKD
HHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2182772; 2969724; 7984428 [H]