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The map label for this gene is celA [H]

Identifier: 157155768

GI number: 157155768

Start: 1734727

End: 1736166

Strand: Reverse

Name: celA [H]

Synonym: EcE24377A_1735

Alternate gene names: 157155768

Gene position: 1736166-1734727 (Counterclockwise)

Preceding gene: 157155300

Following gene: 157159344

Centisome position: 34.87

GC content: 45.21

Gene sequence:

>1440_bases
ATGTCAGGATTTAAAAAAGGTTTTTTATGGGGCGGTGCGGTAGCCGCGCATCAGTTGGAAGGTGGCTGGAATGAAGGAGG
AAAAGGCATCAGCATCGCTGATGTGATGACTGCTGGCGCTCACGGGGTGCCGCGTGAAGTGACAGAAGGCGTTATCGACG
GGCTTAATTATCCCAATCATGAAGCAATTGATTTTTATCATCGCTATAAAACAGATATTCAGTTATTTGCCGAGATGGGA
TTCAAATGCTTTCGAACTTCCATTGCCTGGACACGAATCTTTCCGCAAGGTGACGAACAGGAGCCGAATGAAGAGGGTTT
ACAATTTTATGATGATCTGTTCGATGAATGCCTTAAGCAGGGAATGGAACCTGTGGTGACGCTTTCACATTTTGAGATGC
CTTATCATCTGGTGACAAAATATGGTGGCTGGCGAAACCGTAAACTGATCGACTTTTTTATCCGCTTCGCATCAACGGTC
TTCACGCGCTATAAAGAAAAAGTAAAGTACTGGATGACGTTTAACGAAATCAATAATCAGGTGAATTTCAGCGAAAGCCT
GTGTCCATTTACTAATTCCGGTATCTTGTATTCGCCAGAGGAAGATATCAATGAGCGCGAACAAATAATGTACCAGGCGG
TACATTACGAGTTAGTTGCCAGTGCCCTGGCGGTACAGACTGGAAAATCGATCAATCCTGAATTTAACATTGGCTGTATG
ATCGCCATGTGTCCCATCTATCCTCTGACGTGTGCACCCAACGATATGATGATGGCCACGAAAGCAATGCATCGTCGTTA
CTGGTTTACTGATGTTCATGCTCGTGGATATTATCCGCAACATATGCTGAATTACTTTGCCAGGAAAGGATTCAACCTCG
ATATCACACCAGAAGATAACGCGATTCTTGCCAGTGGTTGTGTCGACTTTATCGGCTTTAGCTACTACATGTCTTTTACG
ACACAATTTTCGCCCGATAACCCGCAACTGGATTATGTTGAACCACGAGATTTGGTCAGCAACCCTTATATCGATACATC
CGAATGGGGATGGCAAATTGATCCGGCAGGGCTACGTTATTCACTCAACTGGTTCTGGGATCATTTCCAGTTGCCGCTGT
TTATTGTCGAAAATGGATTTGGTGCGGTTGACCAGAGACAAGCTGACGGCACGGTGAACGATCACTATCGCATTGATTAC
TTTGCTTCCCATATTCGGGAAATGAAAAAAGCCGTAGTTGAAGATGGTGTTGACTTAATTGGCTACACCCCGTGGGGCTG
CATTGACCTGGTTTCTGCCGGAACAGGGGAAATGAAAAAGCGCTACGGAATGATTTATGTCGACAAAGACAACGAAGGGA
AGGGAACGCTGGAACGGATACGTAAAGCGTCGTTTTACTGGTATCGGGATCTCATCGCCAACAATGGCGAAAATATTTGA

Upstream 100 bases:

>100_bases
ATTATCGAATTTTAAGTTTAATGAGAACAGCAAAGACGAGTTTATGGCCGGCATCCAGGCGGAAGTCTGGTGGTAATCTA
TTTTAGTTAAGGAGTTGTGT

Downstream 100 bases:

>100_bases
GACTTGAGGAAGATATTACGGAGATGTCAGGCTCTGTGATCAGAGCCTGACAATAACTACATAGCGTGTTGATTATAATA
GGGCACGCCGAGCGCATCCG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMG
FKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFT
TQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDY
FASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI

Sequences:

>Translated_479_residues
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMG
FKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFT
TQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDY
FASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
>Mature_478_residues
SGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGF
KCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVF
TRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCMI
AMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTT
QFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYF
ASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=28.8577154308617, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI32481206, Length=493, Percent_Identity=27.3833671399594, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI13273313, Length=492, Percent_Identity=26.0162601626016, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI24497614, Length=491, Percent_Identity=30.142566191446, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=27.2164948453608, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI2367174, Length=475, Percent_Identity=60.2105263157895, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1789070, Length=483, Percent_Identity=48.8612836438923, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI2367270, Length=479, Percent_Identity=50.3131524008351, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17552856, Length=489, Percent_Identity=28.4253578732106, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17539390, Length=488, Percent_Identity=27.6639344262295, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21356577, Length=490, Percent_Identity=28.1632653061224, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55188; Mature: 55057

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNH
CCCCCCCCEECCHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
EAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEINNQ
CCCCEEEHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCC
VNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
CCCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDN
EEECCCCEEECCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCEEECCCCC
AILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRY
CEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEE
SLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLI
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEE
GYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
CCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNH
CCCCCCCEECCHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
EAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEINNQ
CCCCEEEHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCC
VNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
CCCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDN
EEECCCCEEECCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCEEECCCCC
AILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRY
CEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEE
SLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLI
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEE
GYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
CCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125345; 9384377 [H]