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The map label for this gene is yieK

Identifier: 157155622

GI number: 157155622

Start: 4215441

End: 4216163

Strand: Reverse

Name: yieK

Synonym: EcE24377A_4227

Alternate gene names: 157155622

Gene position: 4216163-4215441 (Counterclockwise)

Preceding gene: 157158571

Following gene: 157156784

Centisome position: 84.67

GC content: 49.65

Gene sequence:

>723_bases
ATGAAATTAATCATTACCGAAGATTACCAGGAAATGAGCCGTGTCGCGGCACACCATCTGCTTGGTTATATGTCGAAGAC
GCGTCGTGTTAACCTGGCAATTACCGCCGGTAGCACGCCCAAAGGCATGTATGAATACCTCACCACCCTGGTTAAAGGTA
AGCCCTGGTACGATAACTGCTATTTCTATAATTTCGATGAAATTCCCTTTCGCGGCAAAGAGGGAGAAGGTGTAACGATT
ACCAATCTGCGTAATCTGTTTTTCACCCCTGCGGGGATCAAAGAAGAGAATATCCAGAAGCTCACTATTGATAACTACCG
CGAGCATGATCAGAAACTGGCGCGTGAAGGCGGCCTGGATTTGGTGGTGCTGGGATTAGGTGCAGATGGTCATTTTTGTG
GCAATTTGCCGAATACGACCCATTTCCATGAGCAAACCGTGGAGTTCCCGATCCAGGGAGAGATGGTTGATATTGTGGCG
CATGGTGAGTTGGGCGGCGACTTTTCGTTAGTGCCTGACAGCTACGTGACGATGGGGCCGAAAAGCATCATGGCGGCGAA
AAACTTGCTGATAATTGTCAGTGGCGCAGGTAAAGCGCAGGCACTGAAAAATGTGCTGCAAGGTCCGGTTACCGAAGATG
TTCCAGCTTCCGTGTTGCAGCTGCATCCTTCGCTGATGGTAATCGCTGATAAAGCCGCTGCCGCAGAATTAGCGCTCGGT
TAA

Upstream 100 bases:

>100_bases
ATTACCAGGAATATCCCGGCCTGAACCATGAAATGGATGTCTGGCGGCCTGCCTATGCAGCCTTCGTACAGAAATTATTT
AAATAAAATAAGGCACTAAG

Downstream 100 bases:

>100_bases
GCTGAAGAAATAAGGCCGGATGTATGAATTACGCTCCGGCCTGTTCTCATTATTTAAATAACTACGAAAAATCACCCCAC
AGTCTCAGCTTGCCGCAGTG

Product: putative 6-phosphogluconolactonase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTI
TNLRNLFFTPAGIKEENIQKLTIDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELALG

Sequences:

>Translated_240_residues
MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTI
TNLRNLFFTPAGIKEENIQKLTIDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELALG
>Mature_240_residues
MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTI
TNLRNLFFTPAGIKEENIQKLTIDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELALG

Specific function: Unknown

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family

Homologues:

Organism=Homo sapiens, GI13027378, Length=252, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI19923881, Length=250, Percent_Identity=28.4, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=100, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789530, Length=235, Percent_Identity=26.3829787234043, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786893, Length=251, Percent_Identity=25.8964143426295, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=250, Percent_Identity=27.6, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24581960, Length=251, Percent_Identity=25.8964143426295, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920764, Length=251, Percent_Identity=25.8964143426295, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YIEK_ECOLI (P31470)

Other databases:

- EMBL:   L10328
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_004069.1
- RefSeq:   YP_026242.1
- ProteinModelPortal:   P31470
- SMR:   P31470
- STRING:   P31470
- EnsemblBacteria:   EBESCT00000004331
- EnsemblBacteria:   EBESCT00000016513
- GeneID:   948229
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5613
- KEGG:   eco:b3718
- EchoBASE:   EB1679
- EcoGene:   EG11728
- eggNOG:   COG0363
- GeneTree:   EBGT00050000009779
- HOGENOM:   HBG725991
- OMA:   HYYNFDE
- ProtClustDB:   PRK12358
- BioCyc:   EcoCyc:EG11728-MONOMER
- Genevestigator:   P31470
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280

Pfam domain/function: PF01182 Glucosamine_iso

EC number: NA

Molecular weight: Translated: 26280; Mature: 26280

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 66-66 ACT_SITE 129-129 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKPWYDNC
CEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
YFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYREHDQKLAREGGLD
EEECCCCCCCCCCCCCCEEEEEEHHHEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCE
LVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLVPDSYVTMGP
EEEEEECCCCCEECCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCEEECCCCCEECCC
KSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELALG
HHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHCCC
>Mature Secondary Structure
MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTLVKGKPWYDNC
CEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
YFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYREHDQKLAREGGLD
EEECCCCCCCCCCCCCCEEEEEEHHHEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCE
LVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLVPDSYVTMGP
EEEEEECCCCCEECCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCEEECCCCCEECCC
KSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLMVIADKAAAAELALG
HHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503