Click here to switch to the map view.

The map label for this gene is hycI [H]

Identifier: 157155343

GI number: 157155343

Start: 3005764

End: 3006234

Strand: Reverse

Name: hycI [H]

Synonym: EcE24377A_3005

Alternate gene names: 157155343

Gene position: 3006234-3005764 (Counterclockwise)

Preceding gene: 157154830

Following gene: 157159408

Centisome position: 60.37

GC content: 53.72

Gene sequence:

>471_bases
GTGACTGACGTTTTACTCTGTGTTGGCAATAGCATGATGGGCGATGATGGCGCTGGCCCGCTGCTGGCGGAAAAGTGCGC
CGCCGCGCCGAAAGGTAACTGGGTGGTGATTGACGGCGGTAGTGCACCGGAAAACGACATCGTCGCCATCCGTGAACTGC
GCCCGACACGACTGCTGATTGTCGACGCCACGGATATGGGGCTAAACCCCGGCGAAATCCGCATCATCGACCCGGATGAT
ATCGCCGAGATGTTTATGATGACTACCCATAACATGCCGTTGAACTACCTTATCGACCAGTTGAAAGAAGATATTGGCGA
AGTGATTTTCCTCGGCATCCAGCCGGATATCGTCGGCTTTTACTACCCGATGACCCAGCCGATTAAAGATGCGGTTGAAA
CCGTTTATCAACGACTGGAAGGCTGGGAAGGAAATGGTGGATTTGCGCAGTTAGCGGTGGAAGTAGAGTAG

Upstream 100 bases:

>100_bases
GCGCAAACTTCGCAGCAGCAGGAATGGAGTACAATGCTGCTAAGTATGCTGCATGATATTCATCAGGAAAACGCCATCTA
TTTGATGGTGAGGAGACTGC

Downstream 100 bases:

>100_bases
TTTTTCATTAAGGAATTCAGGGAATTGAACATCAGAACAGGGATGTGCGCTTTTTTATTGAGCTTAGTGCTGCCTGCACA
GGCCACCAGCTTTACCGATT

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE

Sequences:

>Translated_156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE
>Mature_155_residues
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDI
AEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=156, Percent_Identity=99.3589743589744, Blast_Score=314, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 17027; Mature: 16896

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCEEE
YYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCEEE
YYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]