Click here to switch to the map view.

The map label for this gene is higA [H]

Identifier: 157155264

GI number: 157155264

Start: 3004476

End: 3004895

Strand: Reverse

Name: higA [H]

Synonym: EcE24377A_3003

Alternate gene names: 157155264

Gene position: 3004895-3004476 (Counterclockwise)

Preceding gene: 157159408

Following gene: 157157468

Centisome position: 60.34

GC content: 46.43

Gene sequence:

>420_bases
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Upstream 100 bases:

>100_bases
GATGTGCTTTATGGGGTGAACCAGAACTGACTTTTTGCAGAGTGGCTATGAGGGGGATTACGTGCAGACAGACGTCACGT
TACTACAGAGAGAACAAAGA

Downstream 100 bases:

>100_bases
TTTTGACTGATGCCAAACCCCAGATTGCCCGATGCGCTGCGCTTATCAGGCCTTGTATTATCCCTCCAGTGCAGAGAAAA
TCGGCCAGTTTTCTCTGCCT

Product: helix-turn-helix DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Sequences:

>Translated_139_residues
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL
>Mature_138_residues
TANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVG
VALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin [H]

COG id: COG5499

COG function: function code K; Predicted transcription regulator containing HTH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789464, Length=134, Percent_Identity=46.2686567164179, Blast_Score=132, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 15417; Mature: 15285

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLT
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCHHHH
ISHIKALSARFGVKPEWFL
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLT
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCHHHH
ISHIKALSARFGVKPEWFL
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]