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The map label for this gene is treC [H]

Identifier: 157155240

GI number: 157155240

Start: 4802333

End: 4803988

Strand: Reverse

Name: treC [H]

Synonym: EcE24377A_4811

Alternate gene names: 157155240

Gene position: 4803988-4802333 (Counterclockwise)

Preceding gene: 157155820

Following gene: 157157950

Centisome position: 96.47

GC content: 52.96

Gene sequence:

>1656_bases
ATGACTAATCTTCCCCACTGGTGGCAAAACGGCGTTATCTACCAGATTTATCCAAAGAGTTTTCAGGACACCACGGGTAG
CGGCACCGGCGATTTACGTGGCGTTATCCAACGCCTGGACTATCTGCATAAACTGGGCGTTGATGCCATCTGGCTAACCC
CCTTTTATATCTCTCCCCAGGTCGATAACGGTTACGACGTAGCGAACTATACGGCGATTGATCCCACCTACGGCACGCTG
GACGATTTTGACGAACTGGTGACGCAGGCAAAATCGCGCGGGATTCGTATCATTCTCGATATGGTGTTTAACCATACCTC
TACCCAACATGCCTGGTTTCGCGAGGCGCTGAACAAAGAAAGCCCTTACCGCCAGTTTTATATCTGGCGCGATGGCGAAC
CAGAAACGCCACCGAACAACTGGCGTTCAAAATTTGGCGGTAGTGCGTGGCGCTGGCATGCGGAAAGCGAACAGTACTAT
TTGCATCTCTTTGCACCAGAACAGGCGGATCTCAACTGGGAGAATCCAGCGGTACGCGCAGAGCTGAAAAAAGTCTGTGA
ATTCTGGGCCGATCGTGGGGTCGACGGGTTGCGCCTGGATGTGGTGAATCTGATCTCCAAAGACCCGCGTTTCCCTGAAG
ACCTGGATGGCGACGGGCGTCGCTTCTACACCGACGGGCCACGAGCACACGAGTTTTTGCACGAGATGAACCGCGATGTG
TTTACGCCACGCGGGTTAATGACCGTAGGTGAAATGTCCTCCACCAGCCTTGAGCATTGCCAGCGATACGCGGCACTGAC
AGGCAGTGAATTGTCGATGACCTTTAATTTTCATCACCTGAAGGTCGATTATCCCGGTGGTGAAAAATGGACGCTGGCTA
AACCTGACTTTGTGGCGTTGAAAACATTGTTCCGCCACTGGCAACAAGGAATGCACAACGTGGCATGGAATGCCTTGTTC
TGGTGTAACCACGATCAGCCGCGCATTATTTCTCGCTTTGGTGATGAAGGTGAATACCGCGTGCCTGCGGCAAAAATGCT
GGCGATGGTGCTGCATGGCATGCAGGGAACGCCGTATATCTACCAGGGCGAAGAGATTGGCATGACCAACCCGCATTTCA
CGCGCATTACTGACTATCGCGACGTGGAGAGCCTAAATATGTTTGCCGAGCTGCGCAACGATGGTCGTGATGCCGACGAG
TTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGCCGCACGCCCATGCAATGGAGCAACGGCGATAATGCCGGGTT
TACGGCTGGCGAACCGTGGATTGGCCTGGGCGATAACTATCAAAAAATCAACGTAGAAGCCGCGCTGGCCGATGAGTCCT
CGGTGTTTTACACCTACCAAAAGTTAATCGCACTGCGTAAGCAAGAAGCCATCCTGACATGGGGCAATTACCAGGATCTG
CTGCCAAACAGCCCTGTATTGTGGTGCTATCGCCGTGAATGGAAGGGGCAAACCTTGCTGGTCATTGCCAACCTTAGCCG
TGAGATCCAACCCTGGCAGCCAGGGCAAATGCGCGGCAACTGGCAGCTTGTGATGCATAACTACGAAGAAGCCTCACCAC
AACCCTGTGCCATGAATTTACGGCCTTTTGAGGCTGTCTGGTGGTTACAGAAGTAA

Upstream 100 bases:

>100_bases
TCGTTTATCTATCAGCGGAAATACCGCCTGGGCACGCTGGATATTGTTTAATTTTCTTCGGGGCGCAATTGCGCCCCCTC
GCATTTGCAGGAATAACGTA

Downstream 100 bases:

>100_bases
ATCTTCCCTAAGCCCCGGTAATGCCGGTCATTCCGGGGTTTTGTCTGTCAGCATGTTGTTTTTGTTGATTTTTCAACCAG
CAAATTCATTAAAAAATTTA

Product: trehalose-6-phosphate hydrolase

Products: NA

Alternate protein names: Alpha,alpha-phosphotrehalase [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTL
DDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALF
WCNHDQPRIISRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADE
LLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK

Sequences:

>Translated_551_residues
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTL
DDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALF
WCNHDQPRIISRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADE
LLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK
>Mature_550_residues
TNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLD
DFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYL
HLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDVF
TPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFW
CNHDQPRIISRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADEL
LAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDLL
PNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=522, Percent_Identity=29.5019157088123, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI65506891, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI61744481, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI61744475, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI61744479, Length=108, Percent_Identity=36.1111111111111, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI61744483, Length=108, Percent_Identity=36.1111111111111, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI61744477, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1790687, Length=551, Percent_Identity=98.7295825771325, Blast_Score=1144, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=188, Percent_Identity=31.3829787234043, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789995, Length=137, Percent_Identity=32.1167883211679, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25147709, Length=488, Percent_Identity=26.4344262295082, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32565753, Length=527, Percent_Identity=22.9601518026565, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321726, Length=576, Percent_Identity=36.1111111111111, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6322245, Length=578, Percent_Identity=35.9861591695502, Blast_Score=353, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6324416, Length=576, Percent_Identity=36.4583333333333, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6322241, Length=576, Percent_Identity=36.4583333333333, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6322021, Length=576, Percent_Identity=36.4583333333333, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321731, Length=586, Percent_Identity=34.8122866894198, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6319776, Length=586, Percent_Identity=34.641638225256, Blast_Score=327, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24583745, Length=537, Percent_Identity=35.9404096834264, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI221330053, Length=499, Percent_Identity=37.2745490981964, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24583747, Length=545, Percent_Identity=36.1467889908257, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24583749, Length=545, Percent_Identity=36.1467889908257, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24586597, Length=532, Percent_Identity=34.3984962406015, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24586599, Length=578, Percent_Identity=31.8339100346021, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24586587, Length=503, Percent_Identity=33.3996023856859, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24586593, Length=520, Percent_Identity=32.5, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI45549022, Length=529, Percent_Identity=33.2703213610586, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24586589, Length=536, Percent_Identity=33.2089552238806, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24586591, Length=494, Percent_Identity=32.5910931174089, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI281360393, Length=466, Percent_Identity=30.2575107296137, Blast_Score=198, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR012769 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.93 [H]

Molecular weight: Translated: 63902; Mature: 63771

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQ
CCCCCCHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
VDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKE
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCHHHHHHHHHCCC
SPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRA
CCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHH
ELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVAL
CCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCEEEECCCCHHHH
KTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFGDEGEYRVPAAKMLAMVLHGMQGTPYI
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCEE
YQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWS
EECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEC
NGDNAGFTAGEPWIGLGDNYQKINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCHHH
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNL
CCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RPFEAVWWLQK
CCCCCEEEECC
>Mature Secondary Structure 
TNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQ
CCCCCHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
VDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKE
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCHHHHHHHHHCCC
SPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRA
CCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHH
ELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVAL
CCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCEEEECCCCHHHH
KTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFGDEGEYRVPAAKMLAMVLHGMQGTPYI
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCEE
YQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWS
EECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEC
NGDNAGFTAGEPWIGLGDNYQKINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCHHH
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNL
CCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RPFEAVWWLQK
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8083158; 7610040; 9278503; 8421692 [H]