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The map label for this gene is trmA [H]

Identifier: 157155127

GI number: 157155127

Start: 4499282

End: 4500382

Strand: Reverse

Name: trmA [H]

Synonym: EcE24377A_4506

Alternate gene names: 157155127

Gene position: 4500382-4499282 (Counterclockwise)

Preceding gene: 157156398

Following gene: 157158861

Centisome position: 90.38

GC content: 50.23

Gene sequence:

>1101_bases
ATGACCCCCGAACACCTTCCAACAGAACAGTATGAAGCGCAGTTAGCCGAAAAAGTGGTACGTTTGCAAAGTATGATGGC
ACCGTTTTCTGACCTGGTTCCGGAAGTGTTTCGCTCGCCGGTCAGTCATTACCGGATGCGTGCGGAGTTCCGCATCTGGC
ACGATGGCGATGACCTGTATCACATCATTTTCGATCAACAAACCAAAAGCCGCATCCGCGTGGATAGCTTCCCCGCTGCC
AGTGAACTCATCAACCAGTTGATGACGGCGATGATTGCGGGTGTGCGTAATAATCCCGTTCTGCGCCACAAGTTGTTCCA
GATTGATTACCTCACCACGCTGAGTAATCAGGCGGTGGTTTCCCTGTTGTACCATAAAAAGCTGGATGATGAGTGGCGTC
AGGAAGCTGAAGCCCTGCGCGATGCACTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAA
ATCGAGCTGGATCAAGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAG
TTTTACCCAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATT
TACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCACTGGCGCGCAATTTTGATCGGGTATTAGCCACCGAAATC
GCTAAGCCGTCGGTTGCTGCTGCGCAATACAACATCGCAGCTAACCATATTGATAACGTACAAATTATTCGTATGGCGGC
AGAAGAATTTACTCAGGCGATGAATGGTGTACGCGAGTTTAATCGCCTGCAAGGGATAGACTTGAAGAGTTATCAGTGCG
AAACCATTTTTGTCGACCCACCGCGCAGCGGTCTGGACAGTGAAACCGAGAAAATGGTGCAGGCGTATCCACGTATTCTG
TATATCTCCTGCAATCCGGAAACGTTATGCAAGAATCTGGAAACATTAAGCCAGACGCACAAGGTCGAACGTCTGGCTCT
GTTTGATCAGTTCCCCTACACGCACCATATGGAGTGCGGCGTATTACTGACCGCGAAGTAA

Upstream 100 bases:

>100_bases
ACTGTCCAGACGTAGCTCACAAATAGGAATTCATCAAGATCTGGACATCTGATGAGCAATCCCTACAATCGCCGCGTACT
TTAATTTTTCAGGATACATC

Downstream 100 bases:

>100_bases
AACCCATGCCGGATGCGCCAGCATCCGGCATAATACCGATTACTCAGCAACTTCCTGCTTACGGTTACGCATCTTCGCGC
CAATCCAGAACACCATAATG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK

Sequences:

>Translated_366_residues
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAA
SELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRIL
YISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK
>Mature_365_residues
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAAS
ELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKI
ELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEIA
KPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILY
ISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECGVLLTAK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=100, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=183, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24666579, Length=194, Percent_Identity=29.3814432989691, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 41968; Mature: 41836

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHCCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLTAK
EEEEEC
>Mature Secondary Structure 
TPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHEEEEEEEEEECCCCEE
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHH
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHCCCCCCHHHHH
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
HHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
CCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLTAK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA