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The map label for this gene is pqqL [H]

Identifier: 157154979

GI number: 157154979

Start: 1682243

End: 1685026

Strand: Reverse

Name: pqqL [H]

Synonym: EcE24377A_1684

Alternate gene names: 157154979

Gene position: 1685026-1682243 (Counterclockwise)

Preceding gene: 157157857

Following gene: 157156267

Centisome position: 33.84

GC content: 46.7

Gene sequence:

>2784_bases
ATGAGAAACCTCTGTTTCTTACTGACGTTAGTGGCAACTCTGTTGCTCCCCGGGCGGCTGATTGCCGCCGCCTTACCGCA
GGATGAAAAGTTAATTACCGGGCAACTGGGCAATGGCTTGCGATATATGATTTATCCGCATGCTCATCCAAAGGATCAGG
TAAATTTATGGCTGCAAATTCATACCGGTTCATTGCAGGAAGAAGACAATGAGCGCGGCGTGGCTCATTTTGTAGAACAT
ATGATGTTTAACGGCACAAAAACATGGCCGGGTAATAAAGTCATCGAAACATTTGAGTCAATGGGCCTGCGTTTTGGTCG
CGATGTTAATGCCTATACCAGCTATGACGAAACGGTGTATCAGGTGAGTTTGCCGACTACGCAGAAACAAAATCTGCAAC
AAGTGATGGCAATCTTCAGTGAATGGAGTAATGCCGCAACCTTTGAAAAACTCGAAGTAGACGCTGAACGTGGCGTAATT
ACTGAGGAATGGCGTGCCCATCAGGATGCGAAATGGCGCACCTCTCAGGCGCGCCGCCCTTTCCTGCTGGCTAATACCCG
TAATTTAGACCGTGAACCTATCGGCCTGATGGATACTGTCGCCACGGTCACACCGGCACAATTGCGCCAATTTTATCAAC
GCTGGTATCAACCAAATAATATGACCTTTATCGTGGTCGGCGATATCGACAGTAAAGAAGCGCTGGCGCTGATAAAGGAT
AATTTAAGTAAGCTTCCGGCTAACAAAGCAGCTGAAAATCGCGTCTGGCCGACAAAAGCCGAAAACCACCTGCGCTTTAA
TATCATTAATGATAAAGAAAACCGGGTGAACGGCATCGCACTCTATTATCGCCTGCCAATGGTACAAGTGAACGATGAGC
AAAGCTTTATCGAACAAGCTGAATGGAGCATGTTAGTTCAGCTGTTCAATCAACGTCTGCAGGAACGCATACAGTCGGGC
GAGTTGAAGACTATTTCTGGCGGCACTGCGCGCAGCGTTAAAATTGCACCCGATTATCAGTCGCTGTTTTTCCGTGTAAA
TGCACGAGACGATAATATGCAGGATGCTGCGAATGCATTAATGGCAGAGTTGGCAACCATTGATCAGCATGGTTTTTCTG
CTGAAGAACTCGATGATGTCAAATCTACCCGCCTCACCTGGCTGAAAAATGCGGTTGATCAGCAAGCTGAACGTGATTTA
CGTATGCTGACCAGTCGCCTGGCATCCAGCTCATTAAATAATACGCCGTTCTTGTCGCCGGAAGAGACATATCAACTTTC
GAAACGTCTGTGGCAGCAAATTACCGTGCAAAGTCTGGCGGAAAAATGGCAGCAGTTAAGAAAGAACCAGGACGCATTTT
GGGAGCAAATGGTAAACAATGAGCTTGCCGCCAAAAAAGCATTGTCTCCTGCGGCTATCCTGGCGCTGGAAAAAGAGTAC
GCCAACAAAAAGCTGGCGGCTTACATCTTCCCAGGCAGAAATTTATCGTTAACAGTAGACGCTGACCCACAGGCGGAAAT
TAGCAGCAAAGAAACGCTGGCTGAGAATCTGACATCATTAACACTTTCCAATGGTGCCAGGGTTATTCTGGCAAAATCCG
CGGGTGAAGAGCAAAAGCTACAAATTACTGCCGTATCGAATAAAGGCGATTTAAGTTTCCCTGCGCAGCAAAAATCACTT
ATCGCGCTGGCAAATAAAGCAGTAAGCGGAAGCGGCGTTGGTGAGCTCTCCTCTTCCAGCCTGAAACGCTGGAGTGCGGA
AAATTCGGTAACCATGAGCAGTAAAGTCAGTGGCATGAATACGTTGCTCTCCGTTAGCGCGCGGACTAATAACCCTGAAC
CTGGTTTTCAGTTGATTAACCAGCGAATCACCCACAGCACGATTAACGACAATATTTGGGCATCGCTACAAAATGCTCAA
ATTCAGGCGTTGAAAACGCTCGACCAGCGTCCAGCGGAGAAATTCGCCCAGCAGATGTATGAGACGCGCTATGCTGATGA
CCGCACGAAATTACTGCAAGAAAATCAGATTGTACAGTTTACTGCCGCAGATGCGCTGGCTGCCGATCGCCAGTTGTTTT
CATCTCCAGCGGATATCACGTTTGTCATTGTCGGTAATGTCTCAGAAGACAAACTCGTGGCGTTAATTACGCGTTACTTA
GGATCAATCAAACACTCTGATTCGCCATTAGCCGCAGGTAAACCATTAACTCGCGCGACGGACAACGCATCGGTTACTGT
AAAAGAACAAAATGAACCTGTGGCACAGTTTTCACAGTGGAAGCGTTATGATTCCCGGACACCTGTTAATCTGGCGACGC
GTATGGCGCTCGATGCTTTTAACGTCGCACTGGCAAAAGATCTACGTGTTAATATTCGTGAACAGGCATCTGGAGCATAC
AGCGTTTCTTCTCGCCTCTCGGTTGATCCTCAGGCCAAAGATATCAGTCATTTGCTGGCTTTTACTTGTCAACCAGAACG
ACATGATGAACTGTTAACGTTAGCGAATGAAGTGATGGTTAAGCGTCTGGCTAAAGGGATCAGTGAGCAAGAACTGAATG
AATACCAGCAAAACGTTCAGCGCAGCCTCGATATCCAACAGCGTAGCGTTCAACAATTAGCGAACACTATTGTAAATAGT
CTTATTCAATATGACGATCCTGCAGCATGGACTGAGCAGGAGCAATTGTTGAAACAAATGACGGTAGAGAATGTTAACAC
TGCCGTTAAACAATATCTTTCTCATCCGGTAAATACTTATACCGGAGTATTATTGCCAAAATAA

Upstream 100 bases:

>100_bases
CCAGTGGCCGTACTTTCTGGCTTGATGTCAGCATGAAATTTTAATAGTTCGGATTTTACTTAACCGGGTAAATTCGCCCG
GTTTTCGCATGGAGATAATT

Downstream 100 bases:

>100_bases
TAACAGCCCCGTCAACACATCGTTGGCGGGGATTTTAGCAATATTCGCTATTTTTATGTAATAATTTTATAAATGCGTTC
AAAATAATAATCAAGTACTA

Product: M16B family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 927; Mature: 927

Protein sequence:

>927_residues
MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEH
MMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKD
NLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSG
ELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDL
RMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEY
ANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSL
IALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQ
IQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYL
GSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAY
SVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNS
LIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK

Sequences:

>Translated_927_residues
MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEH
MMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKD
NLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSG
ELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDL
RMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEY
ANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSL
IALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQ
IQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYL
GSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAY
SVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNS
LIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK
>Mature_927_residues
MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEH
MMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKD
NLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSG
ELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDL
RMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEY
ANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSL
IALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQ
IQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYL
GSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAY
SVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNS
LIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI46593007, Length=216, Percent_Identity=24.537037037037, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1787770, Length=927, Percent_Identity=99.0291262135922, Blast_Score=1890, Evalue=0.0,
Organism=Escherichia coli, GI1789946, Length=467, Percent_Identity=21.1991434689507, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324474, Length=192, Percent_Identity=26.0416666666667, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 104238; Mature: 104238

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMIYPHAHPKDQVNLWLQI
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEEEE
HTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVY
ECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCEEE
QVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRP
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCHHHHCCCCCCHHHHHCCCC
FLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKD
EEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
NLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQA
HHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECEEEECCHHHHHHHH
EWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANAL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCHHHEEEEEECCCCCHHHHHHHH
MAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHH
ANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKL
CCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCEE
QITAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMN
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHCCHH
TLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMY
HHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHH
ETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYL
HHHCCCHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
GSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAF
HHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
NVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMV
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVENVNTAVKQYLSHPVNTYTGVLLPK
HHHHHHHHHHHHHCCCCHHHCCCEECC
>Mature Secondary Structure
MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMIYPHAHPKDQVNLWLQI
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEEEE
HTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVY
ECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCEEE
QVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRP
EEECCCCCHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCHHHHCCCCCCHHHHHCCCC
FLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKD
EEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
NLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQA
HHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECEEEECCHHHHHHHH
EWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANAL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCHHHEEEEEECCCCCHHHHHHHH
MAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHH
ANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKL
CCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCEE
QITAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMN
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHCCHH
TLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMY
HHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHH
ETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYL
HHHCCCHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
GSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAF
HHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
NVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMV
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVENVNTAVKQYLSHPVNTYTGVLLPK
HHHHHHHHHHHHHCCCCHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]