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The map label for this gene is ansP [H]

Identifier: 157154745

GI number: 157154745

Start: 1624457

End: 1625956

Strand: Reverse

Name: ansP [H]

Synonym: EcE24377A_1635

Alternate gene names: 157154745

Gene position: 1625956-1624457 (Counterclockwise)

Preceding gene: 157158787

Following gene: 157156568

Centisome position: 32.65

GC content: 52.07

Gene sequence:

>1500_bases
ATGAGTAAACACGACACCGACACTTCAGATCAACACGCCGCGAAACGCCGCTGGCTTAATGCCCACGAAGAGGGGTATCA
CAAAGCGATGGGCAATCGCCAGGTACAGATGATCGCCATTGGCGGCGCGATTGGCACCGGCTTGTTTTTAGGTGCAGGAG
CCCGACTGCAAATGGCGGGTCCAGCATTGGCACTGGTTTATTTAATTTGTGGCTTGTTTTCGTTTTTTATTCTGCGTGCA
TTGGGTGAGCTGGTGCTACACCGCCCTTCCAGTGGCAGTTTTGTCTCTTATGCCCGTGAGTTTTTGGGTGAGAAAGCCGC
TTATGTTGCTGGCTGGATGTACTTCATCAACTGGGCGATGACGGGGATTGTTGATATTACCGCCGTCGCTCTGTATATGC
ATTACTGGGGTGCGTTTGGCGGCGTGCCGCAGTGGGTCTTTGCGCTCGCTGCACTTACCATCGTTGGCACCATGAATATG
ATCGGTGTGAAATGGTTTGCGGAGATGGAGTTCTGGTTTGCGCTTATTAAAGTGCTCGCCATTGTGACCTTTTTGGTCGT
GGGTACAGTGTTCCTCGGTAGTGGTCAGCCGCTGGATGGCAACACCACTGGCTTTCATTTAATTACCGATAATGGCGGCT
TCTTCCCCCACGGTTTGCTGCCTGCTCTGGTGTTGATTCAGGGCGTAGTGTTTGCTTTTGCCTCCATTGAAATGGTGGGT
ACAGCTGCCGGAGAATGTAAAGATCCGCAGACCATGGTGCCTAAAGCGATTAACAGCGTGATTTGGCGTATTGGCCTGTT
TTACGTCGGCTCCGTGGTGTTGCTGGTTATGCTATTGCCGTGGAGCGCGTATCAGGCGGGGCAAAGTCCGTTCGTGACGT
TTTTCTCTAAACTGGGTGTGCCATATATCGGCAGCATTATGAACATTGTGGTGCTGACCGCTGCCCTCTCCAGCCTGAAC
TCAGGTCTGTACTGCACCGGACGTATTCTGCGCTCAATGGCGATGGGCGGTTCCGCACCGAGTTTTATGGCGAAAATGAG
TCGTCAGCATGTGCCGTATGCCGGGATTCTGGCGACACTAGTTGTGTATGTCGTCGGCGTATTCCTCAACTATCTGGTGC
CGTCGCGCGTATTTGAGATTGTGTTGAACTTCGCGTCGCTGGGAATCATCGCTTCATGGGCGTTTATCATCGTGTGCCAG
ATGCGCCTGCGTAAAGCAATTAAACAAGGCAAAGCAGCGGATGTCAGTTTTAAACTGCCTGGCGCGCCCTTCACTTCCTG
GCTGACATTACTGTTTTTACTGAGTGTCCTGGTGCTGATGGCGTTCGATTACCCGAACGGGACTTATACTATCGCGGCGC
TGCCGATTATCGGTATGCTGCTGGTTATAGGCTGGTTTGGTGTGCGCAAACGCGTTGCTGAAATTCACAGCACTGCGCCA
GTCGTCGAGGAAGATGAAGAAAAACAGGAAATTGTGTTTAAGCCTGAAACGGCGAGCTAA

Upstream 100 bases:

>100_bases
ACCCATAAAGAATAATGGTGATAACTATCATCGCCAGGATGAATAAACATTGTTCATGGCAACTTATATGACTTTTTCAT
TAAAGCAATCAGGGAGAGCA

Downstream 100 bases:

>100_bases
TTGCAAAATTGCCCGGCTACGTGCCGGGCAAATTTAGATTACTGACGAAGGTGTGCCGCATGCGGCGTGATAAAAGTCAG
GCAAGTCTCTCCGTTTTCAC

Product: L-asparagine permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGMLLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS

Sequences:

>Translated_499_residues
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGMLLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS
>Mature_498_residues
SKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRAL
GELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMI
GVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVGT
AAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNS
GLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQM
RLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGMLLVIGWFGVRKRVAEIHSTAPV
VEEDEEKQEIVFKPETAS

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=435, Percent_Identity=22.9885057471264, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI258645169, Length=415, Percent_Identity=21.6867469879518, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081915, Length=499, Percent_Identity=99.5991983967936, Blast_Score=1004, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=456, Percent_Identity=36.8421052631579, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1786789, Length=439, Percent_Identity=36.6742596810934, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790653, Length=459, Percent_Identity=37.037037037037, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1786602, Length=449, Percent_Identity=36.7483296213808, Blast_Score=298, Evalue=8e-82,
Organism=Escherichia coli, GI48994972, Length=421, Percent_Identity=38.7173396674584, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI87081708, Length=458, Percent_Identity=33.4061135371179, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789017, Length=423, Percent_Identity=33.806146572104, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788480, Length=400, Percent_Identity=34.25, Blast_Score=228, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17533459, Length=436, Percent_Identity=22.4770642201835, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532491, Length=406, Percent_Identity=21.9211822660099, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324061, Length=464, Percent_Identity=29.0948275862069, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320772, Length=438, Percent_Identity=29.4520547945205, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=516, Percent_Identity=31.2015503875969, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324990, Length=522, Percent_Identity=26.8199233716475, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324924, Length=513, Percent_Identity=28.2651072124756, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321629, Length=436, Percent_Identity=28.8990825688073, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=30.8270676691729, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321053, Length=461, Percent_Identity=27.3318872017354, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324059, Length=465, Percent_Identity=28.6021505376344, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6322967, Length=423, Percent_Identity=28.6052009456265, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320717, Length=416, Percent_Identity=28.3653846153846, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324981, Length=423, Percent_Identity=27.8959810874704, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6320251, Length=458, Percent_Identity=26.8558951965066, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319543, Length=424, Percent_Identity=27.5943396226415, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319824, Length=414, Percent_Identity=27.0531400966184, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319608, Length=477, Percent_Identity=25.1572327044025, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319542, Length=410, Percent_Identity=26.3414634146341, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320364, Length=503, Percent_Identity=21.272365805169, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221512776, Length=424, Percent_Identity=21.2264150943396, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24666159, Length=424, Percent_Identity=21.2264150943396, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24668806, Length=428, Percent_Identity=22.196261682243, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356285, Length=428, Percent_Identity=22.196261682243, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24668802, Length=428, Percent_Identity=22.196261682243, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 54251; Mature: 54120

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC
PALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMIGVKWFAEMEFWFALIKVLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCC
PYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
VVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQMRLRKAIKQGKAADVSFKLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGMLLVIGWFGVRKRVAEIHSTAP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEEDEEKQEIVFKPETAS
CCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
SKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC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CCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]