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The map label for this gene is thiE

Identifier: 157154731

GI number: 157154731

Start: 4530595

End: 4531230

Strand: Reverse

Name: thiE

Synonym: EcE24377A_4535

Alternate gene names: 157154731

Gene position: 4531230-4530595 (Counterclockwise)

Preceding gene: 157159265

Following gene: 157156779

Centisome position: 91.0

GC content: 58.18

Gene sequence:

>636_bases
ATGTATCAGCCTGATTTTCCTCCTGTACCTTTTCGTTCAGGACTGTACCCGGTGGTGGACAGCGTACAGTGGATCGAACG
TCTGTTGGATGCAGGCGTACGTACTCTCCAGCTACGCATCAAAGATCGGCGCGATGAAGAGGTGGAGGCCGATGTCGTGG
CGGCAATTGCGCTGGGCCGCCGCTATAACGCGCGATTGTTTATCAACGATTACTGGCGGCTGGCGATCAAGCATCAGGCG
TATGGCGTCCATTTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAATGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGT
TTCGACACATGACGATATGGAAATCGACGTCGCGCTGGCAGCACGCCCCTCTTATATCGCGCTGGGACATGTGTTCCCGA
CGCAAACCAAACAGATGCCTTCTGCACCGCAGGGGCTGGAACAGCTGGCACGGCATGTTGAGCGACTGGCGGATTATCCC
ACCGTGGCGATTGGCGGTATCAGTCTGGCACGCGCGCCTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAG
CGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGA

Upstream 100 bases:

>100_bases
ATTACGCCGCCACGCAAACTATTGAAATGGGAATGGCGGATATGTCGGAGAACTTCCGTGCCAGAGGCGGAGAAATCTAC
CTGCGTAAGGAGGAAGCGTG

Downstream 100 bases:

>100_bases
CCGTGACTTTATGCGTTATAGCCGCCAAATCCTGCTCGACGATATCGCTCTGGACGGGCAGCAAAAACTGCTCGACAGCC
AGGTGCTGATTATCGGTCTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE

Sequences:

>Translated_211_residues
MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE
>Mature_211_residues
MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=100, Blast_Score=422, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_ECO24 (A7ZUK8)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465487.1
- ProteinModelPortal:   A7ZUK8
- SMR:   A7ZUK8
- STRING:   A7ZUK8
- EnsemblBacteria:   EBESCT00000018975
- GeneID:   5589036
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4535
- eggNOG:   COG0352
- GeneTree:   EBGT00050000010388
- HOGENOM:   HBG754477
- OMA:   HAVQPSY
- ProtClustDB:   PRK03512
- BioCyc:   ECOL331111:ECE24377A_4535-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23016; Mature: 23016

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCEEEEEEE
ARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCHHHHCC
GSIAVVSAITQAADWRLATAQLLEIAGVGDE
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCEEEEEEE
ARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCHHHHCC
GSIAVVSAITQAADWRLATAQLLEIAGVGDE
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA